MIB1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA644692A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in: Hela whole cell lysate(30µg), A431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), U251 whole cell lysate(30µg), COLO205 whole cell lysate(30µg) All lanes: MIB1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time：120s IHC image of CSB-RA644692A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA644692A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q86YT6

Target Names

MIB1

Alternative Names

DAPK-interacting protein 1 antibody; Dip 1 antibody; DIP-1 antibody; Dip1 antibody; E3 ubiquitin protein ligase MIB 1 antibody; E3 ubiquitin protein ligase MIB1 antibody; E3 ubiquitin-protein ligase mib1 antibody; KIAA1323 antibody; LVNC7 antibody; MIB antibody; mib1 antibody; MIB1_HUMAN antibody; Mind bomb homolog 1 antibody; Mindbomb E3 ubiquitin protein ligase 1 antibody; Ubiquitin ligase mind bomb antibody; Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody; ZZANK2 antibody; ZZZ6 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human MIB1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MIB1, or Mindbomb E3 ubiquitin protein ligase 1, plays a critical role in Notch signaling pathway regulation through its ubiquitin ligase activity. This enzyme ubiquitinates Notch ligands, promoting their endocytosis and enabling productive Notch receptor activation in neighboring cells. Given the fundamental importance of Notch signaling in cell fate determination, proliferation, and differentiation, MIB1 has become an essential target for researchers investigating developmental biology, stem cell maintenance, and disease mechanisms including certain cardiomyopathies.

This recombinant monoclonal antibody, clone 10A9, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human MIB1, has been affinity-purified to ensure high specificity for your target.

Validation studies demonstrate reliable performance across multiple applications. Western blot analysis confirms detection of MIB1 at the expected 110 kDa molecular weight across diverse human cell lines including HeLa, A431, HEK293, U251, and COLO205, indicating robust performance regardless of cellular context. Immunohistochemistry validation in paraffin-embedded human colorectal cancer tissue shows clear staining with standard citrate buffer antigen retrieval protocols. Flow cytometry analysis using SH-SY5Y neuroblastoma cells demonstrates distinct positive signal separation from isotype controls, confirming utility for single-cell analysis applications.

Whether you are exploring Notch pathway dynamics, investigating stem cell biology, or characterizing MIB1 expression patterns in disease models, this antibody provides the validated performance and experimental flexibility your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

Gene References into Functions

M1B-1 antigen was shown to increase the risk. High birth weight, pesticide exposure (childhood exposure, and parental occupational exposure) and maternal consumption of cured meat during pregnancy may also increase the risk of onset of childhood brain tumours PMID: 27212451
In the absence of PCM1, Mib1 destabilizes Talpid3 through poly-ubiquitylation and suppresses cilium assembly. PMID: 27146717
The aim of the study was to evaluate the caspase-3 and survivin expression in correlation with MIB-1 expression in gliomas of various grade. PMID: 26995334
provided evidences that miR-10 regulates human endothelial cells behaviour through targeting Mib1 as well PMID: 26825552
these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis. PMID: 25795303
Expression of MIB1 ligase protein, human was correlated with tumor grade and Figo stages. PMID: 26204652
Data show how E3 ubiquitin protein ligase Mind bomb protein recognizes notch receptor ligands. PMID: 25747658
a significant survival benefit for p16-positive vaginal cancers compared with p16-negative cancers for stages I and II. No difference was observed in survival for MIB-1-positive tumors PMID: 25319982
Invasive adenomas have a higher inducible nitric oxide synthase, and this correlated with the MIB-1 PMID: 24008756
This is the first published study ever assessing the expression of COX-2, p16 and Ki67 markers in ductal carcinoma in situ breast tumors. PMID: 25077370
Data show that the E3 ubiquitin ligase, mind bomb 1 (Mib1), interacts with and ubiquitinates SMN and facilitates its degradation. PMID: 23615451
MIB-1 was found to be associated with estrogen receptors in the stromal component PMID: 23442362
implicate NOTCH signaling in left ventricular noncompaction and indicate that MIB1 mutations arrest chamber myocardium development, preventing trabecular maturation and compaction PMID: 23314057
MIB1 negatively regulates TNFalpha- and IL1beta-induced NF-kappaB target gene activation PMID: 22184009
RYK interacts both physically and functionally with the E3 ubiquitin MIB1. MIB1 is sufficient to activate Wnt/CTNNB1 signaling and this activity depends on endogenous RYK. PMID: 21875946
Data demonstrate that c-mip interacts with Dip1 and upregulates DAPK, which blocks the nuclear translocation of ERK1/2. PMID: 20018188
Neuralized-2 regulates a Notch ligand in cooperation with Mind bomb-1 PMID: 17003037
DAPK is found in two distinct immune complexes, one containing HSP90 and CHIP and a second complex containing only DIP1/Mib; strict modulation of DAPK activities by HSP90 heterocomplexes is critical for regulation of apoptosis and cellular homeostasis PMID: 17324930
Comparative genomic hybridization and immunohistochemical assessment of EGFR, PTEN, p53, and MIB-1 expression in 13 oligodendrogliomas, one oligoastrocytoma and 23 high-grade astrocytomas is reported. PMID: 17390041
The interaction between Mib1 and cFLIP decreases the association of caspase-8 with cFLIP, which activates caspase-8 and induces cell death PMID: 19710364

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Involvement in disease

Left ventricular non-compaction 7 (LVNC7)

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cell membrane.

Tissue Specificity

Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Database Links

HGNC: 21086

OMIM: 608677

KEGG: hsa:57534

STRING: 9606.ENSP00000261537

UniGene: Hs.140903

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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