Product Details

Uniprot No.

Target Names

Alternative Names

72 kDa gelatinase antibody; 72kD type IV collagenase antibody; CLG 4 antibody; CLG 4A antibody; CLG4 antibody; CLG4A antibody; Collagenase Type 4 alpha antibody; Collagenase type IV A antibody; Gelatinase A antibody; Gelatinase alpha antibody; Gelatinase neutrophil antibody; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody; Matrix Metalloproteinase 2 antibody; Matrix metalloproteinase II antibody; Matrix metalloproteinase-2 antibody; MMP 2 antibody; MMP II antibody; MMP-2 antibody; MMP2 antibody; MMP2_HUMAN antibody; MONA antibody; Neutrophil gelatinase antibody; PEX antibody; TBE 1 antibody; TBE-1 antibody

Species Reactivity

Human

Immunogen

Recombinant Human MMP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

20F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Matrix metalloproteinase-2 (MMP2), also known as gelatinase A or 72 kDa type IV collagenase, plays a central role in extracellular matrix remodeling through its ability to degrade type IV collagen, a major component of basement membranes. This enzymatic activity positions MMP2 as a critical mediator in processes ranging from normal tissue development and wound healing to pathological conditions including tumor invasion, metastasis, and angiogenesis. Understanding MMP2 expression patterns and localization provides valuable insights into disease progression and potential therapeutic targets.

This recombinant monoclonal antibody, clone 20F9, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically to the last, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The antibody was generated against recombinant human MMP2 protein and is validated for human sample detection.

Immunohistochemistry validation demonstrates robust performance across tissue types with distinct biological relevance. Testing in paraffin-embedded human placenta tissue confirms detection in a context where MMP2 contributes to trophoblast invasion and vascular remodeling during implantation. Validation in human prostate cancer tissue showcases the antibody's utility in oncology research, where MMP2 overexpression correlates with invasive potential and disease progression. Both applications performed optimally at 1:50 to 1:200 dilutions using citrate buffer antigen retrieval.

Supplied in a glycerol-based PBS buffer with long-term stability at -20°C or -80°C, this antibody supports researchers investigating tumor microenvironment dynamics, metastatic mechanisms, tissue remodeling disorders, and developmental biology where MMP2-mediated matrix degradation influences cellular behavior and disease outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.; Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Gene References into Functions

No significant associations were observed with either MMP-2(-1306) C/T or MMP-3(-1171) 5A/6A. PMID: 30117779
PROK1 or MMP-2 in the amniotic fluid do not have a role in the prediction of adverse pregnancy outcomes. PMID: 29405963
CDH17 has a role in altering MMP-2 expression via canonical NF-kappaB signalling in human gastric cancer PMID: 29783070
Study results revealed that CCR2 promotes epithelialtomesenchymal transition through MMP2 in liver cancer. PMID: 30132565
results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle PMID: 30187906
Fewer TIMP-2, Hsp70 and TGF-beta1 immunoreactive cells in younger individuals and increased expression of Hsp70 in elderly individuals demonstrated the influence of aging in lung remodeling PMID: 29325453
Melatonin inhibits epithelialtomesenchymal transition in gastric cancer cells via attenuation of IL1beta/NFkappaB/MMP2/MMP9 signaling PMID: 30066836
TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
proteins such as MMP2 and MMP9 as well as P38 expression were also affected by the PKM2 expression changes. These results proved that PKM2 could be involved in the progression of bladder cancer by mitogen-activated protein kinases signaling pathway. PMID: 30249877
High MMP2 expression is associated with colorectal cancer cell migration, invasion and metastasis PMID: 30015978
We conducted a case-control genetic association study, adjusted for known IA risk factors (smoking and hypertension), in a UK Caucasian population of 1409 patients with intracranial aneurysms (IA), and 1290 matched controls, to determine the association of the rs243865 C > T functional MMP-2 gene SNP with IA (overall, and classified as ruptured and unruptured). PMID: 29334797
MMP2 rs243865 and MMP9 rs3918242 polymorphisms are significantly associated with the risk of RSA in Chinese population. PMID: 30290617
findings demonstrate an association between the MMP-2 C-735 T polymorphism with blood pressure and the risk of preeclampsia PMID: 29017408
The role of MMP 2 in asthma remains unclear and no study has been conducted till date, to determine the role of MMP-2 -735C/T gene polymorphism in asthma. This study does not disprove such association. PMID: 28051223
knockdown of TRIM31 reduced the expression of MMP2, MMP9 and phospho-Akt. PMID: 29864908
SB22055 could reduce the expression of BSP, OPN and MMP2 in prostate cancer cells. PMID: 29917166
Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea skeletal fluorosis. PMID: 28079131
MMP-2 genetic variants are an important mediator of stroke risk PMID: 30278505
HMGB1 promoted lung cancer invasion and metastasis by upregulating the expression and activity of MMP-2 in an NF-kappaB-dependent manner. PMID: 29850505
High glucose levels induce MMP-2 expression in Human cardiac fibroblasts , possibly by up-regulation of SGLT1. PMID: 29512713
we have discovered the novel N-72, and it was crucial for EGF-induced migration by targeting MMP2 in Human amnion mesenchymal stem cells (hAMSCs) PMID: 29734654
data indicated that MMP-2/MMP-9 was coordinately targeted by two non-coding RNAs, miR-296-3p and FOXCUT, which were decreased, and tumor-suppressing factors in choroidal malignant melanoma PMID: 29260433
Increased MMP-2 and MMP-9 activities may contribute to the pathogenesis and disease severity of cutaneous lupus erythematosus. PMID: 29349515
No association between the carriage of studied MMP2 polymorphisms and the predisposition to preeclampsia was found. PMID: 29670668
We propose that serum levels of MMP-2 and MMP-9 are not predictive on treatment response and survival in LARC patients. PMID: 29578156
that miR-338-5p has a function in promoting glioma cell invasion by targeting TSHZ3 suppression on MMP2 PMID: 28780604
In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. PMID: 29504111
OSM [oncostatin M]might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322
TIMP2 promotes tumor progression and miR2055p directly regulates TIMP2, thereby suppressing proMMP2 activation and inhibiting oral squamous cell carcinoma cell invasiveness. PMID: 29393341
Elevated MMP-2 levels were observed in blood of pancreatic cancer patients which correlated with its tissue expression. However, these levels did not associate with survival or any clinicopathological parameters of patients. PMID: 29355139
Increased urinary concentration of matrix metallopeptidase 2 (MMP-2) at 12 and 16 weeks of gestation predicted an increased risk of developing preeclampsia in the study population. PMID: 28055277
There was no difference found in MMP-2, MMP-9 or TLR-4 levels between non-thrombocytopenic and thrombocytopenic septic donors. PLA formation was increased in thrombocytopenic patients. PMID: 29734352
MMP2 rs243865 was the only single nucleotide polymorphism significantly associated with Floppy Mitral Valve/Mitral Valve Prolapse (FMV/MVP) as compared to the control.The frequency of certain MMP2 polymorphisms is higher in patients with the FMV/MVP syndrome and patients with FMV/MVP without the syndrome. PMID: 28750369
Platelets may contribute to joint degeneration in knee osteoarthritis by favouring the accumulation of MMP-2 in synovial fluid. PMID: 28981555
In pregnancy MMP2 gene expression was significantly higher in preterm than full term. Human papilloma viral load was found to be positively correlated to the rate of MMP2 expression and the gestational age was significantly related to the viral load and the rate of expression of MMP2 gene. Human pabilloma virus especially high-risk genotypes was correlated to spontaneous preterm labor in Egyptian females. PMID: 28264621
We conclude that S1P attenuates the invasion of C643 cells by activating S1P2 and the Rho-ROCK pathway, by decreasing calpain activity, and by decreasing the expression, secretion and activity of MMP2 and, to a lesser extent, MMP9. Our results thus unveil a novel function for the S1P2 receptor in attenuating thyroid cancer cell invasion. PMID: 29734379
High mmp2 expression is associated with ovarian cancer cell migration and invasion. PMID: 28677781
MMP-2 was expressed in high percentage of endometrial cancer and its expression may be associated closely with clinical stage, and tumor invasion and metastasis, indicating that MMP-2 overexpression may serve as a predictive factor for poor prognosis of endometrial cancer. PMID: 30024495
MMP2 single nucleotide polymorphisms association with lymphedema caused by Wuchereria bancrofti. PMID: 29122006
MMP-2 promoted and MMP-13 disrupted vasculogenic mimicry formation in large cell lung cancer by cleaving laminin-5 to influence EGFR signal activation. PMID: 28766880
Data suggest that environmental carcinogen PFOA (perfluorooctanoic acid) stimulates ovarian cancer cell migration, invasion, and MMP2/MMP9 expression by up-regulating ERK/NFkappaB signaling pathway. (MMP = matrix metallopeptidase; NFkappaB = nuclear factor kappa B) PMID: 29753068
Matrix-metalloproteinase-2 is implicated in the pathophysiological mechanism of stenosis development and has a predictive value for arteriovenous fistula failure in hemodialysis patients PMID: 28868755
Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
Rictor regulates the vasculogenic mimicry of melanoma and determines the patients' survival via the AKT-MMP2-MMP9 pathway. PMID: 28699701
Expression of BRM and MMP2 in the thoracic aortic aneurysm and aortic dissection is very high, indicating that BRM and MMP2 may play important roles in the occurrence and development of thoracic aortic aneurysm and aortic dissection. PMID: 28678310
at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
MMP-2 serum level and circulating tumor cells show the potential to predict CNS metastases and overall survival in breast cancer patients; CTCs and MMP-9 serum level could be a promising therapy response marker in castration resistant prostate cancer patients PMID: 28529342
RhoGDIbeta overexpression led to downregulation of miR-200c, whereas miR-200c was able directly to target 3'-UTR of jnk2mRNA and attenuated JNK2 protein translation, which resulted in attenuation of Sp1mRNA and protein expression in turn, inhibiting Sp1-dependent MMP-2 transcription. PMID: 28846829
both HBEGF upregulation and apoptosis were rescued by exogenous MMP2 PMID: 28731464
MMP-2 (and MMP-1 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037

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Involvement in disease

Multicentric osteolysis, nodulosis, and arthropathy (MONA)

Subcellular Location

[Isoform 1]: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.; [Isoform 2]: Cytoplasm. Mitochondrion.

Protein Families

Peptidase M10A family

Tissue Specificity

Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Database Links

HGNC: 7166

OMIM: 120360

KEGG: hsa:4313

STRING: 9606.ENSP00000219070

UniGene: Hs.513617

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Code	CSB-RA014666MA1HU
Size	$49.9
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Image	IHC image of CSB-RA014666MA1HU diluted at 1：50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA014666MA1HU diluted at 1：50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
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MMP2 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background