Human Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Code CSB-E04675h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Matrix metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit is suitable for the quantitative detection of MMP-2 levels in human serum, plasma, cell culture supernates, and tissue homogenates. The detection mechanism of this kit is based on the sandwich ELISA, in which the formed pre-coated MMP-2 antibody/MMP-2/Biotin-conjugated MMP-2 antibody complex is labeled by HRP-avidin and then develops a color reaction after the addition of the TMB substrate solution. The color intensity can be measured at 450 nm via a microplate reader. Many excellent performances of this kit, including sensitivity, specificity, precision, recovery, and linearity, have been rigorously validated. Refer to the product instructions for more validation information.

MMP-2 is a kind of gelatinase A capable to degrading collagen, elastin, fibronectin, lamin, and gelatin. It also exerts pro-inflammatory and anti-inflammatory effects on numerous tissues. MMP-2 is constitutively expressed in several tissues and regulated by TNF-α under the influence of NF-κB. And MMP-2 plays a protective role in hypertensive heart disease by inhibiting the transcription and activity of 3-hydroxy-3-methylglutaryl-CoA reductase in the early stages of the hypertensive response.

Target Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Alternative Names 72 kDa gelatinase ELISA Kit; 72kD type IV collagenase ELISA Kit; CLG 4 ELISA Kit; CLG 4A ELISA Kit; CLG4 ELISA Kit; CLG4A ELISA Kit; Collagenase Type 4 alpha ELISA Kit; Collagenase type IV A ELISA Kit; Gelatinase A ELISA Kit; Gelatinase alpha ELISA Kit; Gelatinase neutrophil ELISA Kit; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase ELISA Kit; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit; Matrix Metalloproteinase 2 ELISA Kit; Matrix metalloproteinase II ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP 2 ELISA Kit; MMP II ELISA Kit; MMP-2 ELISA Kit; MMP2 ELISA Kit; MMP2_HUMAN ELISA Kit; MONA ELISA Kit; Neutrophil gelatinase ELISA Kit; PEX ELISA Kit; TBE 1 ELISA Kit; TBE-1 ELISA Kit
Abbreviation MMP2
Uniprot No. P08253
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-92  
1:2 Average % 99  
Range % 91-107  
1:4 Average % 96  
Range % 92-101  
1:8 Average % 93  
Range % 86-112  
Recovery
The recovery of human MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 80-98  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
50 2.659 2.588 2.624 2.435  
25 2.210 2.080 2.145 1.956  
12.5 1.540 1.580 1.560 1.371  
6.25 1.021 1.009 1.015 0.826  
3.12 0.588 0.620 0.604 0.415  
1.56 0.390 0.420 0.405 0.216  
0.78 0.250 0.260 0.255 0.066  
0 0.190 0.188 0.189    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MMP-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled MMP-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.; Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Gene References into Functions
  1. No significant associations were observed with either MMP-2(-1306) C/T or MMP-3(-1171) 5A/6A. PMID: 30117779
  2. PROK1 or MMP-2 in the amniotic fluid do not have a role in the prediction of adverse pregnancy outcomes. PMID: 29405963
  3. CDH17 has a role in altering MMP-2 expression via canonical NF-kappaB signalling in human gastric cancer PMID: 29783070
  4. Study results revealed that CCR2 promotes epithelialtomesenchymal transition through MMP2 in liver cancer. PMID: 30132565
  5. results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle PMID: 30187906
  6. Fewer TIMP-2, Hsp70 and TGF-beta1 immunoreactive cells in younger individuals and increased expression of Hsp70 in elderly individuals demonstrated the influence of aging in lung remodeling PMID: 29325453
  7. Melatonin inhibits epithelialtomesenchymal transition in gastric cancer cells via attenuation of IL1beta/NFkappaB/MMP2/MMP9 signaling PMID: 30066836
  8. TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
  9. proteins such as MMP2 and MMP9 as well as P38 expression were also affected by the PKM2 expression changes. These results proved that PKM2 could be involved in the progression of bladder cancer by mitogen-activated protein kinases signaling pathway. PMID: 30249877
  10. High MMP2 expression is associated with colorectal cancer cell migration, invasion and metastasis PMID: 30015978
  11. We conducted a case-control genetic association study, adjusted for known IA risk factors (smoking and hypertension), in a UK Caucasian population of 1409 patients with intracranial aneurysms (IA), and 1290 matched controls, to determine the association of the rs243865 C > T functional MMP-2 gene SNP with IA (overall, and classified as ruptured and unruptured). PMID: 29334797
  12. MMP2 rs243865 and MMP9 rs3918242 polymorphisms are significantly associated with the risk of RSA in Chinese population. PMID: 30290617
  13. findings demonstrate an association between the MMP-2 C-735 T polymorphism with blood pressure and the risk of preeclampsia PMID: 29017408
  14. The role of MMP 2 in asthma remains unclear and no study has been conducted till date, to determine the role of MMP-2 -735C/T gene polymorphism in asthma. This study does not disprove such association. PMID: 28051223
  15. knockdown of TRIM31 reduced the expression of MMP2, MMP9 and phospho-Akt. PMID: 29864908
  16. SB22055 could reduce the expression of BSP, OPN and MMP2 in prostate cancer cells. PMID: 29917166
  17. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea skeletal fluorosis. PMID: 28079131
  18. MMP-2 genetic variants are an important mediator of stroke risk PMID: 30278505
  19. HMGB1 promoted lung cancer invasion and metastasis by upregulating the expression and activity of MMP-2 in an NF-kappaB-dependent manner. PMID: 29850505
  20. High glucose levels induce MMP-2 expression in Human cardiac fibroblasts , possibly by up-regulation of SGLT1. PMID: 29512713
  21. we have discovered the novel N-72, and it was crucial for EGF-induced migration by targeting MMP2 in Human amnion mesenchymal stem cells (hAMSCs) PMID: 29734654
  22. data indicated that MMP-2/MMP-9 was coordinately targeted by two non-coding RNAs, miR-296-3p and FOXCUT, which were decreased, and tumor-suppressing factors in choroidal malignant melanoma PMID: 29260433
  23. Increased MMP-2 and MMP-9 activities may contribute to the pathogenesis and disease severity of cutaneous lupus erythematosus. PMID: 29349515
  24. No association between the carriage of studied MMP2 polymorphisms and the predisposition to preeclampsia was found. PMID: 29670668
  25. We propose that serum levels of MMP-2 and MMP-9 are not predictive on treatment response and survival in LARC patients. PMID: 29578156
  26. that miR-338-5p has a function in promoting glioma cell invasion by targeting TSHZ3 suppression on MMP2 PMID: 28780604
  27. In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. PMID: 29504111
  28. OSM [oncostatin M]might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322
  29. TIMP2 promotes tumor progression and miR2055p directly regulates TIMP2, thereby suppressing proMMP2 activation and inhibiting oral squamous cell carcinoma cell invasiveness. PMID: 29393341
  30. Elevated MMP-2 levels were observed in blood of pancreatic cancer patients which correlated with its tissue expression. However, these levels did not associate with survival or any clinicopathological parameters of patients. PMID: 29355139
  31. Increased urinary concentration of matrix metallopeptidase 2 (MMP-2) at 12 and 16 weeks of gestation predicted an increased risk of developing preeclampsia in the study population. PMID: 28055277
  32. There was no difference found in MMP-2, MMP-9 or TLR-4 levels between non-thrombocytopenic and thrombocytopenic septic donors. PLA formation was increased in thrombocytopenic patients. PMID: 29734352
  33. MMP2 rs243865 was the only single nucleotide polymorphism significantly associated with Floppy Mitral Valve/Mitral Valve Prolapse (FMV/MVP) as compared to the control.The frequency of certain MMP2 polymorphisms is higher in patients with the FMV/MVP syndrome and patients with FMV/MVP without the syndrome. PMID: 28750369
  34. Platelets may contribute to joint degeneration in knee osteoarthritis by favouring the accumulation of MMP-2 in synovial fluid. PMID: 28981555
  35. In pregnancy MMP2 gene expression was significantly higher in preterm than full term. Human papilloma viral load was found to be positively correlated to the rate of MMP2 expression and the gestational age was significantly related to the viral load and the rate of expression of MMP2 gene. Human pabilloma virus especially high-risk genotypes was correlated to spontaneous preterm labor in Egyptian females. PMID: 28264621
  36. We conclude that S1P attenuates the invasion of C643 cells by activating S1P2 and the Rho-ROCK pathway, by decreasing calpain activity, and by decreasing the expression, secretion and activity of MMP2 and, to a lesser extent, MMP9. Our results thus unveil a novel function for the S1P2 receptor in attenuating thyroid cancer cell invasion. PMID: 29734379
  37. High mmp2 expression is associated with ovarian cancer cell migration and invasion. PMID: 28677781
  38. MMP-2 was expressed in high percentage of endometrial cancer and its expression may be associated closely with clinical stage, and tumor invasion and metastasis, indicating that MMP-2 overexpression may serve as a predictive factor for poor prognosis of endometrial cancer. PMID: 30024495
  39. MMP2 single nucleotide polymorphisms association with lymphedema caused by Wuchereria bancrofti. PMID: 29122006
  40. MMP-2 promoted and MMP-13 disrupted vasculogenic mimicry formation in large cell lung cancer by cleaving laminin-5 to influence EGFR signal activation. PMID: 28766880
  41. Data suggest that environmental carcinogen PFOA (perfluorooctanoic acid) stimulates ovarian cancer cell migration, invasion, and MMP2/MMP9 expression by up-regulating ERK/NFkappaB signaling pathway. (MMP = matrix metallopeptidase; NFkappaB = nuclear factor kappa B) PMID: 29753068
  42. Matrix-metalloproteinase-2 is implicated in the pathophysiological mechanism of stenosis development and has a predictive value for arteriovenous fistula failure in hemodialysis patients PMID: 28868755
  43. Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
  44. Rictor regulates the vasculogenic mimicry of melanoma and determines the patients' survival via the AKT-MMP2-MMP9 pathway. PMID: 28699701
  45. Expression of BRM and MMP2 in the thoracic aortic aneurysm and aortic dissection is very high, indicating that BRM and MMP2 may play important roles in the occurrence and development of thoracic aortic aneurysm and aortic dissection. PMID: 28678310
  46. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
  47. MMP-2 serum level and circulating tumor cells show the potential to predict CNS metastases and overall survival in breast cancer patients; CTCs and MMP-9 serum level could be a promising therapy response marker in castration resistant prostate cancer patients PMID: 28529342
  48. RhoGDIbeta overexpression led to downregulation of miR-200c, whereas miR-200c was able directly to target 3'-UTR of jnk2mRNA and attenuated JNK2 protein translation, which resulted in attenuation of Sp1mRNA and protein expression in turn, inhibiting Sp1-dependent MMP-2 transcription. PMID: 28846829
  49. both HBEGF upregulation and apoptosis were rescued by exogenous MMP2 PMID: 28731464
  50. MMP-2 (and MMP-1 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037

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Involvement in disease Multicentric osteolysis, nodulosis, and arthropathy (MONA)
Subcellular Location [Isoform 1]: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.; [Isoform 2]: Cytoplasm. Mitochondrion.
Protein Families Peptidase M10A family
Tissue Specificity Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Database Links

HGNC: 7166

OMIM: 120360

KEGG: hsa:4313

STRING: 9606.ENSP00000219070

UniGene: Hs.513617

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