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| Code | CSB-RA918477A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
CUSABIO meticulously produced the PLEKHA1 recombinant monoclonal antibody through a rigorous and precise process. B cells were isolated from the spleen of an immunized animal, using a synthesized peptide derived from human PLEKHA1 as the immunogen. RNA was extracted from the B cells and converted into cDNA through reverse transcription. The gene encoding the PLEKHA1 antibody was then amplified using a degenerate primer and inserted into a vector, which was introduced into host cells through transfection, enabling efficient expression of the PLEKHA1 recombinant monoclonal antibodies. After expression, the antibodies were harvested from the cell culture supernatant and underwent purification using affinity chromatography. This antibody has been validated for use in ELISA and WB applications to detect the human PLEKHA1 protein.
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