PPP1CB Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA167614A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg),SH-SY5Y whole cell lysate(30µg),U-251MG whole cell lysate(30µg),A549 whole cell lysate(30µg),Hela whole cell lysate(30µg),A431 whole cell lysate(30µg),Jurkat whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: PPP1B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time：1min IHC image of CSB-RA167614A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA167614A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA167614A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA167614A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P62140

Target Names

PPP1CB

Alternative Names

MGC3672 antibody; PP 1B antibody; PP-1B antibody; PP1B antibody; PP1B_HUMAN antibody; PP1beta antibody; PPP1CB antibody; PPP1CD antibody; Protein phosphatase 1 beta antibody; Protein phosphatase 1 catalytic subunit beta isoform antibody; Protein phosphatase 1 delta antibody; Protein phosphatase 1, catalytic subunit, beta isozyme antibody; Protein phosphatase 1, catalytic subunit, delta isoform antibody; Serine threonine protein phosphatase PP1 beta catalytic subunit antibody; Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PPP1CB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Protein phosphatase 1 catalytic subunit beta (PPP1CB) serves as a critical regulator of cellular signaling, functioning as one of the major serine/threonine phosphatases that counterbalances kinase activity across diverse biological processes. This enzyme participates in glycogen metabolism, cell cycle progression, muscle contractility, and neuronal signaling, making it an essential target for researchers investigating phosphorylation-dependent regulatory networks and disease mechanisms.

This recombinant monoclonal antibody, generated from rabbit host and produced using recombinant technology, offers the consistency and reproducibility that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies. The monoclonal nature of clone 14E4 provides single-epitope recognition, delivering reliable results across extended research programs.

Validation data demonstrates robust performance across multiple applications and sample types. Western blot analysis detects a clean band at the predicted 37 kDa molecular weight across an extensive panel of human cell lines including HT-29, SH-SY5Y, U-251MG, A549, HeLa, A431, and Jurkat cells. Cross-species reactivity with mouse and rat is confirmed through successful detection in brain tissue lysates from both species, expanding utility for translational studies and animal model research. Immunohistochemistry validation in human glioma cancer and small intestine tissue sections demonstrates effective performance in FFPE samples. Additional characterization includes immunofluorescence staining in HeLa cells showing clear cytoplasmic localization, while flow cytometry analysis confirms suitability for single-cell applications.

This antibody supports investigations into phosphatase biology, cancer signaling, neurodegeneration research, and metabolic regulation where PPP1CB function is implicated.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.

Gene References into Functions

This report gives further support that this novel RASopathy-PPP1CB-related Noonan syndrome with loose anagen hair-shares great similarity to Noonan syndrome-like disorder with loose anagen hair, and expands the phenotypic spectrum by adding the cranial vault abnormality. PMID: 28211982
Missense mutations in PPP1CB are associated with a phenotype resembling NS-LAH. Based on this phenotype and PPP1CB's known role within the RAS/MAPK pathway, we think that this condition is a novel rasopathy, which may be provisionally termed "PPP1CB-related Noonan syndrome with loose anagen hair" (P-NS-LAH). PMID: 27264673
Our data suggest that our heterozygous de novo PPP1CB pathogenic variants are associated with syndromic intellectual disability. PMID: 27681385
These results suggest a unique functional role for the PP1beta isoform in affecting cardiac contractile function PMID: 26334248
Findings show that PP1cbeta plays a role in endothelial cell migration through a mechanism involving the interplay of actin cytoskeleton proteins and focal adhesion molecules signaling. PMID: 25967976
In the title. PMID: 25849655
results indicate that SCN-iPS cells provide a useful disease model for SCN, and the activation of the Wnt3a/beta-catenin pathway may offer a novel therapy for SCN with ELANE mutation PMID: 23382248
Interaction between protein phosphatase 1 beta and myosin phosphatase (MYPT)1 results in exclusion of Nkx2.5 from the cell nucleus. PMID: 23168335
These findings provide evidence for the involvement of a particular PP1 complex, PPP1R12A/PP1cdelta, in insulin signaling. PMID: 22728334
Protein phosphatase 1beta (PP1beta) is identified as a phosphatase for the cluster of phosphorylated threonines ((353)TTETQRT(359)) within the sst(2A) somatostatin receptor carboxyl terminus that mediates beta-arrestin binding using siRNA knock-down screening. PMID: 21795688
PPP1CB is over expressed in malignant melanoma. PMID: 21566537
Data suggest that double-thiophosphorylation of TIMAP has minor effect on its binding ability to PP1c, but considerably attenuates its inhibitory effect on the activity of PP1c. PMID: 21466834
serine/threonine protein phosphatase PP1beta is a physiological Nucleophosmin (NPM) phosphatase under both the genotoxic stress and growth conditions. PMID: 20962268
In this study, we show that protein phosphatase-1 (PP1) inhibitor-3 (Inh3) is localized to the nucleoli and centrosomes in interphase HEK 293 cells. PMID: 16256067
R16A at the membrane may mediate the PB signal to initiate CAR nuclear translocation, through a mechanism including its dimerization and inhibition of PP1beta activity. PMID: 18202305

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Involvement in disease

Noonan syndrome-like disorder with loose anagen hair 2 (NSLH2)

Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus.

Protein Families

PPP phosphatase family, PP-1 subfamily

Database Links

HGNC: 9282

OMIM: 600590

KEGG: hsa:5500

STRING: 9606.ENSP00000296122

UniGene: Hs.702907

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

cAMP signaling pathway Hippo signaling pathway Focal adhesion Regulation of actin cytoskeleton Vascular smooth muscle contraction Cellular senescence cGMP-PKG signaling pathway Insulin signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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