PPP2R5D Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA439668A0HU
Size	US$210
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Image	IHC image of CSB-RA439668A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA439668A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA439668A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q14738

Target Names

PPP2R5D

Alternative Names

2A5D_HUMAN antibody; B'delta antibody; B56D antibody; Delta isoform of regulatory subunit B56; protein phosphatase 2A antibody; MGC2134 antibody; MGC8949 antibody; OTTHUMP00000039821 antibody; PP2A B subunit B' delta isoform antibody; PP2A B subunit B56 delta isoform antibody; PP2A B subunit isoform B''-delta antibody; PP2A B subunit isoform B56-delta antibody; PP2A B subunit isoform PR61-delta antibody; PP2A B subunit isoform R5-delta antibody; PP2A B subunit PR61 delta isoform antibody; PP2A B subunit R5 delta isoform antibody; PPP2R5D antibody; Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody; Protein phosphatase 2 regulatory subunit B delta isoform antibody; Protein phosphatase 2 regulatory subunit B' delta antibody; Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody; TEG-271 antibody; Tex271 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PPP2R5D

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Gene References into Functions

Mutations in PPP2R5D are genetic cause of intellectual disability. PMID: 26576547
MiR-9 regulates GR signaling and steroid-resistant airway hyperresponsiveness by reducing protein phosphatase 2A activity. PMID: 25772595
Mutant B56delta was A and C binding-deficient, while mutant Aalpha subunits bound B56delta well but were unable to bind C PMID: 26168268
PP2A-B56delta is the single most significant contributor, of the PP2A holoenzyme subunits, to BubR1 and Bub1 kinetochore localization. PMID: 25246613
Protein kinase A mediated activation of protein phosphatase 2A is enabled by PPP2R5D phosphorylation, which modulates the affinity of the protein phosphatase 2A holoenzyme to its physiological substrates. PMID: 20423611
Data show that the protein phosphatase 2A subunit B56delta specifically interacts with HAND1 and -2, but not other basic helix-loop-helix proteins. PMID: 14636580
Gonadotropin-releasing hormone (GnRH) affects the membrane protein phosphatase 2A-associated apoptosis and the enzyme activity in ovarian cancer cells. PMID: 15809743
PP2A:B56delta as a key upstream regulator of Cdk1 activity upon exit from mitosis PMID: 18056802

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Involvement in disease

Mental retardation, autosomal dominant 35 (MRD35)

Subcellular Location

Cytoplasm. Nucleus. Note=Nuclear in interphase, nuclear during mitosis.

Protein Families

Phosphatase 2A regulatory subunit B56 family

Tissue Specificity

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

Database Links

HGNC: 9312

OMIM: 601646

KEGG: hsa:5528

STRING: 9606.ENSP00000417963

UniGene: Hs.533308

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PI3K-Akt signaling pathway AMPK signaling Sphingolipid signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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