Product Details

Uniprot No.

Q9NZ42

Target Names

PSENEN

Alternative Names

Gamma-secretase subunit PEN-2 (Presenilin enhancer protein 2), PSENEN, PEN2

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PSENEN protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PSENEN, also known as PEN-2 (Presenilin enhancer protein 2), serves as an essential component of the gamma-secretase complex, a multi-subunit protease responsible for the intramembrane cleavage of numerous type I transmembrane proteins. This processing activity is particularly significant in Alzheimer's disease research, where gamma-secretase mediates the final cleavage step in amyloid precursor protein processing to generate amyloid-beta peptides. Beyond neurodegeneration, PSENEN plays critical roles in Notch signaling pathways that regulate cell fate decisions, making it a target of interest across developmental biology and oncology research.

This recombinant monoclonal antibody, generated from clone 16E3 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect reliable performance across experiments and between lots, eliminating the variability concerns associated with traditional hybridoma-derived antibodies.

Validation studies demonstrate robust performance across multiple platforms. Western blot analysis detects a clean 12 kDa band matching the predicted molecular weight, confirmed in diverse human cell lines including A549, THP-1, SH-SY5Y, Colo205, PC-3, A431, and MCF-7. Cross-species reactivity extends to mouse and rat brain tissue lysates, supporting translational studies using rodent models. Immunohistochemistry validation in paraffin-embedded human pancreatic cancer tissue shows specific staining, while flow cytometry analysis in MCF-7 cells demonstrates clear population separation from isotype controls.

This antibody supports researchers investigating gamma-secretase biology, neurodegenerative disease mechanisms, Notch pathway regulation, and cancer biology where aberrant gamma-secretase activity contributes to disease progression.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity.

Gene References into Functions

High gamma-secretase expression is associated with head and neck squamous cell carcinoma. PMID: 29047105
APP substrate occupancy of these three pockets of gamma-secretase occurs after initial substrate binding but precedes catalysis, suggesting a conformational change in substrate may be required for cleavage. PMID: 27580372
PSENEN mutations can indeed cause a comanifestation of Dowling-Degos disease and acne inversa (AI) that is likely triggered by predisposing factors for AI. PMID: 28287404
zinc, copper inhibited Abeta production by directly targeting the subunits presenilin and nicastrin in the gamma-secretase complex PMID: 28096459
PSENEN may play a crucial role in the progression of atopic dermatitis by participating in the Notch signaling pathway. PMID: 26967585
Remarkably, PEN-2 was identified besides nicastrin as additional substrate-binding subunit. PMID: 27220847
Results suggest deltaOR-Phe27Cys variation modulates beta- and gamma-secretase activity in late-stage Alzheimer's disease likely via post-translational mechanisms PMID: 26402014
TRPC6 specifically interacts with APP leading to inhibition of its cleavage by gamma-secretase and reduction in Abeta production. PMID: 26581893
secondary mutations in presenilin 1 alone activated the gamma-secretase activity. PMID: 26559975
Both human PS2V and zebrafish PS1IV can stimulate gamma-secretase activity despite extreme structural divergence PMID: 25814654
Data indicate that familial Alzheimer's disease (FAD) and control brain samples had similar overall gamma-secretase activity levels, and therefore, loss of overall (endopeptidase) gamma-secretase function cannot be an essential part of the pathogenic mechanism. PMID: 26481686
We for the first time identify PEN-2 as the causative gene of familial comedones. PMID: 26044244
The first hydrophobic domain of Pen-2 forms a structure similar to a reentrant loop while the second hydrophobic domain spans the lipid bilayer. PMID: 26296997
shedding of BCMA by gamma-secretase controls plasma cells in the bone marrow and yields a potential biomarker for B-cell involvement in human autoimmune diseases PMID: 26065893
Tumor necrosis factor-alpha and interleukin-10 levels were elevated in acne inversa patients with nicastrin or presenilin enhancer mutation. PMID: 26067312
SLC2A13 is a novel gamma-secretase associated protein that regulates amyloid beta production without affecting Notch cleavage. PMID: 26094765
Brain proteins showing neuron-specific interactions with gamma-secretase PMID: 25893612
A complete inhibition of PS1-induced apoptosis was achieved by knockdown of PS1-associated protein (PSAP), a mitochondrial proapoptotic protein that forms a complex with Bax upon induction of apoptosis, in the presence of gamma-secretase inhibitor. PMID: 26025363
analysis of how the conformation of presenilin, Pen-2, Aph-1, and nicastrin affect the function and mechanism of gamma-secretase PMID: 25918421
Mutation of on PS1 and PS2 AXXXAXXXG motifs strongly impacts gamma-secretase activity. PMID: 25614624
The catalytic subunit of gamma-secretase is presenilin 1 (PS1), which contains an initial substrate-binding site that is distinct from the catalytic site PMID: 25673856
Recombinant human Pen-2 fusion protein was purified from bacteria to >95% purity. PMID: 24865334
Together, our results suggest that iron can increase gamma-secretase activity through promoting the level of FTL that interacts with and stabilizes PEN-2/ PMID: 23685131
A review of mutations in the gamma-secretase genes NCSTN, PSENEN, and PSEN1 and the role of gamma-secretase in cutaneous biology and, more specifically in hidradenitis suppurativa. PMID: 23096707
Allele A of Pen 2 gene may increase the risk of late onset Alzheimer's disease. PMID: 23134962
a 269 bp region located between the PSENEN and U2AF1L4 human genes is a genuine bidirectional promoter regulating a concerted divergent transcription of these genes. PMID: 23246698
Mutations in the gamma-secretase genes NCSTN, PSENEN, and PSEN1 underlie rare forms of hidradenitis suppurativa (acne inversa). PMID: 22622421
The molecular state of gamma-secretase and its enzymological characteristics are described. PMID: 22787762
secretase subunits restrict the arrangement of the transmembrane domains of presenilin during the formation of the functional structure of the catalytic pore. PMID: 22689582
Studies indicate that gradual saturation of gamma-secretase with its substrate can be the pathogenic process in different alleged causes of Alzheimer's disease (AD). PMID: 22479317
expression of calsenilin leads to a disruption of presenilin 1/gamma-secretase-mediated epsilon-cleavage of N-cadherin, which results in the significant accumulation of N-cadherin C-terminal fragment 1 PMID: 21852538
NCSTN and PSENEN, are involved in the pathogenesis of some familial hidradenitis suppurativa (Acne Inversa). PMID: 21412258
structural analysis of PEN-2 conformation by single-particle electron microscopy PMID: 21454611
This study support a gamma-secretase-independent role of presenilin-1 in modulation of filamin-mediated actin cytoskeleton. PMID: 20847418
study found independent loss-of-function mutations in PSENEN, PSEN1, or NCSTN in 6 Chinese acne inversa (AI) families; results identify the gamma-secretase component genes as culprits for a subset of familial AI PMID: 20929727
Data show that intramembranous cleavage by gamma-secretase and related intramembrane-cleaving proteases may generally occur via stepwise endoproteolysis. PMID: 20534834
Hematopoietic gamma-secretase has reduced activity for APP and Notch1 processing compared to epithelial gamma-secretase. PMID: 20178366
Transactivation of the Pen2 promoter by presenilin 1/2 is p53-dependent. PMID: 19889971
a component of the gamma-secretase complex PMID: 12198112
role in regulating proteolytic processing of presenilin 1 in conjunction with APH-1 PMID: 12522139
examination of membrane topology PMID: 12639958
APH-1 stabilizes the presenilin holoprotein in the complex, whereas PEN-2 is required for endoproteolytic processing of presenilin and conferring gamma-secretase activity to the complex. PMID: 12660785
Expression of PEN2 increases amyloid beta peptide levels and gamma-secretase activity. PMID: 12763021
presenilin 1 (PS1)-derived fragments, mature nicastrin, APH-1, and PEN-2, associate with cholesterol-rich detergent insoluble membrane (DIM) domains of non-neuronal cells and neurons PMID: 15322084
the sequence and length of the C terminus of PEN-2 are critical for intermolecular interactions and function of presenilin complexes PMID: 15322109
The presenilin-subunit stabilizing function of PEN-2 depends on length and overall sequence of its carboxyl-terminal domain. PMID: 15953349
knockdown of ubiquilin-1 and -2 protein expression by RNAi (RNA interference) increased Pen-2 and nicastrin levels PMID: 15975090
mutational analyses revealed that the "NF" sequence within the TMD4 of PS1 is the minimal motif that is required for binding with PEN-2, promoting PS1 endoproteolysis and gamma-secretase activity PMID: 16234243
Pen-2 may contribute to the activation of the gamma-secretase complex by directly binding to the TMD4 of PS1 PMID: 16234244
COX-2 may be a downstream effector of mutant N141I PS2-mediated apoptotic cell death and that inhibition of COX-2 may neuroprotect in AD through modulation of a GSK-3beta-beta-catenin-mediated response. PMID: 16331303

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Involvement in disease

Acne inversa, familial, 2 (ACNINV2)

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.

Protein Families

PEN-2 family

Tissue Specificity

Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.

Database Links

HGNC: 30100

OMIM: 607632

KEGG: hsa:55851

STRING: 9606.ENSP00000222266

UniGene: Hs.534465

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Code	CSB-RA927089A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), colo205 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), A431 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: PEN2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time：2min IHC image of CSB-RA927089A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA927089A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PSENEN Recombinant Monoclonal Antibody

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Target Background