Phospho-GATA3 (S308) Antibody

Datasheet
Code CSB-RA009276A308phHU
Size US$350
Uniprot No. P23771
Abbreviation Trans-acting T-cell-specific transcription factor GATA-3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate
    All lanes:Phospho-GATA3 antibody at 2.5ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 48 KDa
    Observed band size: 48 KDa

  • IHC image of CSB-RA009276A308phHU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen A synthesized peptide derived from human Phospho-GATA3 (S308)
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Immunology
Gene Names GATA3
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Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Involvement in disease Hypoparathyroidism, sensorineural deafness, and renal disease (HDR)
Subcellular location Nucleus
Pathway Th1 and Th2 cell differentiation
Th17 cell differentiation

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