Alternative Names
AI413597 antibody; AW045860 antibody; DDPAC antibody; FLJ31424 antibody; FTDP 17 antibody; G protein beta1/gamma2 subunit interacting factor 1 antibody; MAPT antibody; MAPTL antibody; MGC134287 antibody; MGC138549 antibody; MGC156663 antibody; Microtubule associated protein tau antibody; Microtubule associated protein tau isoform 4 antibody; Microtubule-associated protein tau antibody; MSTD antibody; Mtapt antibody; MTBT1 antibody; MTBT2 antibody; Neurofibrillary tangle protein antibody; Paired helical filament tau antibody; Paired helical filament-tau antibody; PHF tau antibody; PHF-tau antibody; PPND antibody; PPP1R103 antibody; Protein phosphatase 1, regulatory subunit 103 antibody; pTau antibody; RNPTAU antibody; TAU antibody; TAU_HUMAN antibody; Tauopathy and respiratory failure antibody; Tauopathy and respiratory failure, included antibody
Immunogen
A synthesized peptide derived from Human Phospho-MAPT (S324)
Immunogen Species
Homo sapiens (Human)
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA, IF
Recommended Dilution
Application |
Recommended Dilution |
IF |
1:20-1:200 |
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
To produce the phospho-MAPT (S324) recombinant monoclonal antibody, the initial step involves isolating the genes responsible for coding the MAPT antibody from rabbits that have been immunized with a synthesized peptide derived from the human MAPT protein phosphorylated at S324. These antibody genes are then cloned into specialized expression vectors. Following this genetic modification, the vectors are introduced into host suspension cells. Subsequently, positive cells are cultured to facilitate the expression and secretion of antibodies. The phospho-MAPT (S324) recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. Finally, the antibody's functionality is rigorously tested through ELISA and IF assays, confirming its ability to react with human MAPT protein phosphorylated at S324.