Product Details

Uniprot No.

Target Names

Alternative Names

Dmrpa1 antibody; Drosophila Replication Protein A antibody; DRPA antibody; HSSB antibody; Human single stranded DNA binding protein antibody; MST075 antibody; MSTP075 antibody; p70 antibody; REPA1 antibody; Replication factor A antibody; Replication factor A protein 1 antibody; Replication protein A 70 kDa DNA-binding subunit antibody; Replication protein A 70kDa DNA binding subunit antibody; Replication protein A1 70kDa antibody; Replication protein A1 antibody; RF A antibody; RF-A protein 1 antibody; RFA antibody; RFA1_HUMAN antibody; RP A antibody; RP-A p70 antibody; RPA 70 antibody; RPA antibody; rpa1 antibody; Single stranded binding protein 70 antibody; Single-stranded DNA-binding protein antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human RPA1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Replication Protein A1 (RPA1), also known as the 70 kDa DNA-binding subunit of the RPA complex, serves as an essential component of DNA replication, repair, and recombination machinery. As the largest subunit of the heterotrimeric RPA complex, RPA1 binds single-stranded DNA with high affinity, protecting it from nuclease degradation while coordinating the recruitment of downstream repair and replication factors. This central role in maintaining genomic stability makes RPA1 a critical target for researchers investigating DNA damage responses, cell cycle regulation, and cancer biology.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human RPA1, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations, ensuring your results remain comparable across experiments.

Validation across multiple platforms demonstrates the versatility of this antibody for diverse experimental approaches. Western blot analysis confirms robust detection in human cell lines including K562, Jurkat, U-251MG, HeLa, and HEK293, with an observed band at approximately 70 kDa—slightly above the predicted 68 kDa molecular weight, consistent with post-translational modifications commonly observed for this protein. Immunofluorescence staining in A-431 cells reveals clear nuclear localization patterns, while flow cytometry analysis in Jurkat cells shows distinct positive population shifts compared to isotype controls.

Whether you are exploring replication stress responses, characterizing DNA repair pathway dynamics, or investigating RPA1 dysregulation in disease contexts, this antibody provides a reliable foundation for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Gene References into Functions

RPA serves to stimulate the primase activity of PrimPol. PMID: 28534480
These new findings supports the existence of a functional PrimPol/RPA association that allows repriming at the exposed ssDNA regions formed in the leading strand upon replicase stalling. PMID: 28396594
regulatory pathway based on casein kinase 2-G9a-RPA permits homologous recombination in cancer cells PMID: 28698370
RPA1 serves as an oncogene during gastrointestinal cancers progression PMID: 29601890
RPA1 is significantly up-regulated in hepatocellular carcinoma and correlates with poor prognosis. RPA1 influences cell cycle through CDK4/Cyclin-D pathway. PMID: 29477843
PCAF/GCN5-mediated lysine 163 acetylation of RPA1 is crucial for nucleotide excision repair. PMID: 28854354
the acetylation status of RPA1 played a crucial role in repair of DNA damage via nucleotide excision repair. PMID: 28854355
data obtained allow us to suggest that XPA can be involved in the post-incision NER stages via its interaction with RPA PMID: 29320546
The results suggest that RPA phosphorylation enhances the recruitment of PRP19 to RPA-ssDNA and stimulates RPA ubiquitylation through a process requiring both PRP19 and RFWD3, thereby triggering a phosphorylation-ubiquitylation circuitry that promotes ATR activation and homologous recombination. PMID: 28666352
RPA recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation. PMID: 28107649
RPA is a sensor of R loops and a regulator of RNaseH1, extending the versatile role of RPA in suppression of genomic instability. PMID: 28257700
Results suggest that the UNG2 N-terminus may serve as a flexible scaffold to tether PCNA and RPA at the replication fork, and that post-translational modifications on the UNG2 N-terminus disrupt formation of the PCNA-UNG2-RPA protein complex. PMID: 28746850
Data suggest that, during human DNA replication, restricting PCNA (proliferating cell nuclear antigen) to the vicinity of its DNA target site is important; PCNA can be maintained at or near primer/template junctions during DNA synthesis by RPA (replication protein A) or SSB (single-stranded DNA-binding protein); here, the SSB used was from Escherichia coli. PMID: 28590137
Strikingly, the addition of the single-stranded DNA (ssDNA)-binding replication protein A (RPA) selectively restores XPF-ERCC1 endonuclease activity on this structure. The 5'-3' exonuclease SNM1A can load from the XPF-ERCC1-RPA-induced incisions and digest past the crosslink to quantitatively complete the unhooking reaction. PMID: 28607004
RPA deficiency induces activation of the Fanconi anemia pathway in an ATR-dependent manner, which may play a role in the genome maintenance. PMID: 27398742
Force regulated dynamics of RPA1 on a DNA fork during DNA replication has been reported. PMID: 27016742
By monitoring DNA double-strand breaks mis-repair using a sensitive bioassay, it was found that depletion of homologous recombination proteins, including BRCA2, BRCA1 or RPA1, resulted in a distinct mutational signature associated with significant increases in break-induced mutation frequencies. PMID: 27131361
results reveal that ETAA1 is a novel RPA-interacting protein that promotes restart of stalled replication forks. PMID: 27601467
miR-30a targets the DNA replication protein RPA1, hinders the replication of DNA and induces DNA fragmentation. PMID: 27208176
show that RPA can bind on each side of the G-quadruplex but it unwinds the G4 only from 5' toward 3'. PMID: 27440048
Replication protein A binds tightly to the single-stranded DNA adjacent to a blocked primer/template junction and restricts PCNA to the upstream duplex region by physically blocking diffusion of PCNA along ssDNA. PMID: 28177605
CTF18 forms a complex with RPA when replication stress is elicited by hydroxyurea treatment or UV exposure during S phase. The interaction kinetics between CTF18 and RPA is positively associated with the phosphorylation status of Chk1. PMID: 27175616
ATRIP deacetylation by SIRT2 promotes ATR-ATRIP binding to replication protein A-single-stranded DNA to drive ATR activation and thus facilitate recovery from replication stress. PMID: 26854234
radiation induced the excessive expression RPA1 in TE-1 cells, and the radiosensitivity of TE-1R cells was less than that of TE-1 cells PMID: 26824734
Our study predicted that UBC and RPA had potential as target genes for the diagnosis and treatment of osteosarcoma PMID: 26782416
FAN1 efficiently promoted DNA incision at the proper site of RPA-coated 5'-flapped DNA. Therefore, FAN1 possesses the ability to promote the ICL repair of 5'-flapped DNA covered by RPA. PMID: 25922199
a significant increase in DKC1, RAD50, MRE11 and RPA1 expression in MM cases with high bone marrow infiltration (pPMID: 26366868
Here, we introduce a number of key characteristics and concepts, including the modularity of the proteins, linkage of weak binding sites, direct competition between sites, and allostery, using the protein replication protein A (RPA). [review] PMID: 25542993
RFWD3-dependent ubiquitination of RPA regulates repair at stalled replication forks. PMID: 26474068
RPA70 is an intrinsically highly dynamic single-stranded DNA-binding complex during both replication and distinct steps of nucleotide excision repair. PMID: 25453469
by targeting RPA and mimicking DNA, DSS1 functions with BRCA2 in a two-component homologous recombination mediator complex in genome maintenance and tumor suppression PMID: 26145171
The D228Y mutation in human RPA1 affects its DNA-binding activity to telomeric DNA. PMID: 26041456
RPA plays an important maintaining genome integrity. [Review] PMID: 25400143
RPA by itself does not affect pol eta dependent lesion bypass fidelity when copying either 8-oxoG or T-T CPD lesions. PMID: 24824831
Both human RPA and hepatitis C virus NS5A(S25-C447), but not NS5A(S25-K215), enabled the NS5BDelta21-NS3 helicase complex to be stably associated with the template and synthesize RNA product in a highly processive manner in vitro. PMID: 25320291
In addition to its annealing helicase activity, which eliminates the natural binding substrate for RPA, HARP blocks the phosphorylation of RPA by DNA-PK. PMID: 24565939
Fanconi anemia group J (FANCJ) helicase partners with the single-stranded DNA-binding protein replication protein A (RPA) to displace BamHI-E111A bound to duplex DNA in a specific manner. PMID: 24895130
LT prevents recruitment of RPA to nuclear foci after DNA damage. This leads to failure to recruit repair proteins such as Rad51 or Rad9, explaining why LT prevents repair of double strand DNA breaks by homologous recombination. PMID: 24204272
these results suggest that the PSO4 complex functionally interacts with RPA and plays an important role in the DNA damage response. PMID: 24443570
the current understanding of RPA's roles in replication by reviewing the available structural data, DNA-binding properties, interactions with various replication proteins, and interactions with DNA repair proteins when DNA replication is stalled. PMID: 22918586
ATR-mediated suppression of dormant origins shields active forks against irreversible breakage via preventing exhaustion of nuclear RPA. This study elucidates how replicating genomes avoid destabilizing DNA damage. PMID: 24267891
Data suggest that replication protein A (RPA) brings a complex of SMARCAL1 and WRN to stalled forks, but that they may act in different pathways to promote fork repair and restart. PMID: 23671665
Results reveal a mechanism for the crosstalk between HR repair and NHEJ through the co-regulation of p53-RPA interaction by DNA-PK, ATM and ATR. PMID: 22797063
RPA is a cofactor of the BLM-Topo IIIalpha-RMI1-RMI2 complex in double holliday junction dissolution PMID: 23543748
Depletion of RPA1 abolishes HSF1 access to the promoter of HSP70 in unstressed condition and delays its rapid activation in response to heat shock. PMID: 22940245
In vitro analysis of the role of replication protein A (RPA) and RPA phosphorylation in ATR-mediated checkpoint signaling. PMID: 22948311
The radiosensitizing effect of DPYD depletion plus CPT was the additive effect of DPYD depletion and CPT. PMID: 22510597
Replication stress-induced recruitment of HDHB to chromatin is independent of checkpoint signaling but correlates with the level of replication protein A (RPA) recruited to chromatin. PMID: 22194613
cells require different RPA functions in DNA replication and DNA repair. PMID: 22179778
Study propose that RPA plays a role in the protection of the human genome cell from A3G and other deaminases when they are inadvertently diverged from their natural targets. PMID: 21935481

Hide All

Subcellular Location

Nucleus. Nucleus, PML body.

Protein Families

Replication factor A protein 1 family

Database Links

HGNC: 10289

OMIM: 179835

KEGG: hsa:6117

STRING: 9606.ENSP00000254719

UniGene: Hs.461925

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA072639A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: K562 whole cell lysate(20µg),Jurkat whole cell lysate(20µg),U-251MG whole cell lysate(20µg),Hela whole cell lysate(20µg),HEK293 whole cell lysate(20µg) All lanes: RPA p70 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 70 kDa Exposure time：2min Immunofluorescence staining of A-431 cell with CSB-RA072639A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA072639A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

RPA1 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background