Product Details

Uniprot No.

Target Names

Alternative Names

40S ribosomal protein S3 antibody; fb13d09 antibody; FLJ26283 antibody; FLJ27450 antibody; IMR 90 ribosomal protein S3 antibody; MGC56088 antibody; MGC87870 antibody; OTTHUMP00000229804 antibody; OTTHUMP00000229805 antibody; OTTHUMP00000229874 antibody; OTTHUMP00000229877 antibody; OTTHUMP00000229878 antibody; OTTHUMP00000229879 antibody; OTTHUMP00000229880 antibody; OTTHUMP00000229882 antibody; OTTHUMP00000229883 antibody; OTTHUMP00000229886 antibody; Ribosomal protein S3 antibody; rps3 antibody; RS3_HUMAN antibody; S3 antibody; wu:fb13d09 antibody; zgc:56088 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human RPS3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25D5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RPS3, or 40S ribosomal protein S3, serves as an essential component of the small ribosomal subunit and plays multifunctional roles that extend well beyond protein synthesis. This protein participates in DNA repair pathways, apoptosis regulation, and has been implicated in various cellular stress responses, making it a valuable marker for researchers investigating ribosome biogenesis, translational control, and cell survival mechanisms.

This recombinant monoclonal antibody, clone 25D5, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthesized peptide from human RPS3, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format and affinity-chromatography purification further contribute to reliable performance across experiments.

Validation by western blot demonstrates robust detection of RPS3 at the expected 27 kDa molecular weight across multiple human cell lines, including MCF7, HEK293, THP-1, and Jurkat cells. This broad performance across epithelial, embryonic kidney, monocytic, and T-cell lineages confirms the antibody's utility for diverse experimental models within human cell biology research. The recommended working dilution range of 1:500 to 1:5000 for western blot applications provides flexibility to optimize signal intensity based on your specific sample conditions and detection systems.

Whether you are examining ribosomal protein expression patterns, investigating translational regulation, or exploring the non-canonical functions of RPS3 in DNA damage response, this antibody delivers the specificity and batch consistency needed for meaningful, reproducible results in cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Involved in translation as a component of the 40S small ribosomal subunit. Has endonuclease activity and plays a role in repair of damaged DNA. Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA. Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS). Has also been shown to bind with similar affinity to intact and damaged DNA. Stimulates the N-glycosylase activity of the base excision protein OGG1. Enhances the uracil excision activity of UNG1. Also stimulates the cleavage of the phosphodiester backbone by APEX1. When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage. Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide. Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes. Represses its own translation by binding to its cognate mRNA. Binds to and protects TP53/p53 from MDM2-mediated ubiquitination. Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization. Involved in induction of apoptosis through its role in activation of CASP8. Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5. Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation.

Gene References into Functions

Results found that BfrB subverts the host innate immune system by binding the NF-kappaB subunit RPS3 and promotes the survival of mycobacteria in macrophages by inhibiting cytokine production in host cells. PMID: 29018126
These results reveal that RPS3 upregulates XIAP independently of the NF-kappaB pathway in human breast cancer cells PMID: 29048653
Asn 165 residue of rpS3 is a critical site for N-linked glycosylation and passage through the ER-Golgi secretion pathway. PMID: 27384988
findings suggest that uS3 residing in the 40S ribosome might perform extra-ribosomal functions related to control of DNA quality PMID: 28334742
Short 5'UTR mRNAs are enriched with TISU (translation initiator of short 5'UTR), a 12-nucleotide element directing efficient scanning-independent translation. This study demonstrate that TISU is particularly dependent on eukaryotic initiation factor 1A (eIF1A) which interacts with both RPS3 and RPS10e. PMID: 28584194
Data show that ribosomal protein S3 (RPS3) knockdown decreased mitochondrial calcium uptake 1 protein (MICU1) expression. PMID: 26336993
Increased RPS3 expression is associated with osteosarcoma invasion. PMID: 25449781
RPS3, a component of basic translation machinery operates at initiation and most probably elongation of protein synthesis, is also implicated in various events of the cell life as an extraribosomal player. [Review] PMID: 24239944
These findings suggest that the secreted rpS3 protein is an indicator of malignant tumors. PMID: 24211576
rpS3 accumulates in the mitochondria to repair damaged DNA due to the decreased interaction between rpS3 and HSP90 in the cytosol. PMID: 23911537
rpS3 acts as a microtubule associated protein and regulates spindle dynamics during mitosis. PMID: 23131551
A novel radioresistance mechanism through functional orchestration of rpS3, TRAF2, and NF-kappaB in non-small cell lung cancer cells, is reported. PMID: 23188828
rpS3 is recruited to the DISC and plays a critical role in both genotoxic stress and cytokine induced apoptosis. PMID: 22510408
The phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase. PMID: 21871177
rpS3 is covalently modified by SUMO-1 and this post-translational modification regulates rpS3 function by increasing rpS3 protein stability. PMID: 21968017
Data show that the IKKbeta-dependent modification of a specific amino acid in RPS3 promoted specific NF-kappaB functions that underlie the molecular pathogenetic mechanisms of E. coli O157:H7. PMID: 21399639
PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kappaB and MAP kinase, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation. PMID: 20709134
when Flag-tagged rpS3 was transiently transfected into 293T cells, the level of endogenous rpS3 gradually decreased regardless of transcription PMID: 20217897
DNA pull-down assays using a 7,8-dihydro-8-oxoguanine duplex oligonucleotide as a substrate found that RPS3 acted as a scaffold for the additional binding of MDM2 and p53. PMID: 19656744
Electron paramagnetic resonance study reveals a putative iron-sulfur cluster in human rpS3 protein. PMID: 11911468
RPS3 is involved in apoptosis. PMID: 14988002
Using surface plasmon resonance technology, the authors show that ribosomal protein S3 positively interacts with the human base excision repair enzymes N-glycosylase/apurinic-apyrimidinic lyase OGG1 and APE/Ref-1. PMID: 15518571
The S3-K132A mutant retained the ability to cleave abasic DNA, but its capacity to bind 8-oxoG was abrogated completely. PMID: 16737853
PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer PMID: 17140567
S3 is a key protein at the mRNA binding site neighboring mRNA downstream of the codon at the decoding site in the human ribosome. PMID: 17179743
hRpS3 may be involved in the uracil-excision pathway, probably by participating in the DNA repair mechanism to remove uracil generated by the deamination of cytosine in DNA, and by preventing C/G-->T/A transition mutations. PMID: 18973764
Protein S3 fragments neighboring mRNA during elongation and translation termination on the human ribosome PMID: 19088750
these results clearly show that arginine methylation of rpS3 plays a critical role in its import into the nucleolus, as well as in small subunit assembly of the ribosome. PMID: 19460357

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, nucleolus. Mitochondrion inner membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton, spindle.

Protein Families

Universal ribosomal protein uS3 family

Database Links

HGNC: 10420

OMIM: 600454

KEGG: hsa:6188

STRING: 9606.ENSP00000433821

UniGene: Hs.546286

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Code	CSB-RA031953A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:MCF7 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), Jurkat whole cell lysate(30µg) All lanes: RPS3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time：180s
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RPS3 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background