RPS6KB2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA949737A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: p70 S6 kinase beta 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 53 kDa Observed band size: 63 kDa Exposure time：2min IHC image of CSB-RA949737A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA949737A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing K562 cells stained with CSB-RA949737A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9UBS0

Target Names

RPS6KB2

Alternative Names

Ribosomal protein S6 kinase beta-2 (S6K-beta-2) (S6K2) (EC 2.7.11.1) (70 kDa ribosomal protein S6 kinase 2) (P70S6K2) (p70-S6K 2) (S6 kinase-related kinase) (SRK) (Serine/threonine-protein kinase 14B) (p70 ribosomal S6 kinase beta) (S6K-beta) (p70 S6 kinase beta) (p70 S6K-beta) (p70 S6KB) (p70-beta), RPS6KB2, STK14B

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RPS6KB2, also known as p70 S6 kinase beta-2, is a serine/threonine kinase that plays a central role in mTOR signaling and translational control. As a key downstream effector of the PI3K/Akt/mTOR pathway, RPS6KB2 phosphorylates ribosomal protein S6 to regulate protein synthesis, cell growth, and proliferation. Its involvement in metabolic regulation and its frequent dysregulation in cancer make it an important target for researchers investigating oncogenic signaling, metabolic disorders, and therapeutic intervention strategies.

This recombinant monoclonal antibody, generated from clone 11D12 in rabbit host, offers the consistency and reproducibility that demanding research applications require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures sequence-defined specificity and eliminates lot-to-lot variability, giving you confidence that your results will remain comparable across extended studies.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis detects RPS6KB2 in diverse human cell lines including HeLa, HepG2, COLO-205, Jurkat, MCF-7, and A431, with an observed band at approximately 63 kDa. The difference from the predicted 53 kDa molecular weight likely reflects post-translational modifications such as glycosylation or phosphorylation events characteristic of this kinase. Immunohistochemistry validation in human placenta tissue confirms reliable detection in fixed specimens, while immunofluorescence staining in MCF-7 cells and flow cytometry analysis in K562 cells demonstrate versatility for cellular localization and quantitative studies.

Whether you are investigating mTOR pathway dynamics, characterizing kinase activity in cancer models, or exploring metabolic signaling networks, this antibody provides a dependable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.

Gene References into Functions

Overexpression of catalytically-active Akt or knockdown of glycogen synthase kinase-3 (GSK3)-beta, a substrate for Akt, had little effect on Mcl-1 downregulation caused by S6K2 deficiency PMID: 28301598
We propose that the S6K2/TRBP node controls miRNA biogenesis in HDLECs and provides a molecular link between the mTOR pathway and the miRNA biogenesis machinery. PMID: 27407113
discovered that ERBB4 and S6K2 were the direct targets of miR-193a-3p and that PIK3R3 and mTOR were the direct targets of miR-193a-5p in non-small-cell lung cancer PMID: 24469061
Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
The mTOR effectors 4EBP1 and S6K2 are frequently coexpressed, and associated with a poor prognosis and endocrine resistance in breast cancer. PMID: 24131622
The p85 S6K1 promotes H2O2-induced cell death via a rapamycin-insensitive mechanism. PMID: 22955948
S6K2 amplification was frequently observed in gastric cancer and was related to a poor prognosis PMID: 23393338
Study shows that the two homologues of S6K have distinct effects on Akt activation and cell survivalin breast cancer. PMID: 21427355
heterogeneous ribonucleoprotein F is involved in the regulation of cell proliferation via the mammalian target of rapamycin/S6 kinase 2 pathway PMID: 20308064
p70 S6 kinase regulates neutrophilic differentiation in HL-60 cells PMID: 12176053
regulation of nucleocytoplasmic shuttling of S6KbetaII by protein kinase C-mediated phosphorylation PMID: 12529391
the PI3K/p70 S6K/c-Myc cascade plays an important role in neutrophilic proliferation in HL-60 cells. PMID: 12818373
These data suggest that activated p70 S6 kinase could mediate an up-regulation of tau translation. PMID: 12875979
p70 ribosomal protein S6 kinase activity was induced by K-Ras in a phosphatidylinositol 3-kinase and mTOR-dependent manner. PMID: 14729629
S6K2 is active throughout the cell cycle with higher activity in G2 and M phases. PMID: 17786541

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily

Database Links

HGNC: 10437

OMIM: 608939

KEGG: hsa:6199

STRING: 9606.ENSP00000308413

UniGene: Hs.534345

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

ErbB signaling pathway HIF-1 signaling pathway mTOR signaling pathway PI3K-Akt signaling pathway TGF-beta signaling pathway Autophagy Fc gamma R-mediated phagocytosis AMPK signaling Apelin signaling pathway Insulin signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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