Code | CSB-RA155372A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
FC | 1:20-1:200 |
To produce the SKP2 recombinant monoclonal antibody, the first step is to obtain the SKP2 monoclonal antibody-encoding gene. B cells were isolated from the animal immunized with a synthesized peptide derived from human SKP2 and were subsequently fused with myeloma cells to generate hybridomas. The SKP2 antibody-producing hybridomas were screened, and the variable light and variable heavy domains of the antibody were sequenced and cloned into a vector. The vector containing the SKP2 monoclonal antibody gene was then transfected into cells for cultivation, and the SKP2 recombinant monoclonal antibody was purified using affinity chromatography from the cell culture supernatant. This SKP2 recombinant monoclonal antibody has been specifically tested and found to react with human SKP2 samples in ELISA, IF, and FC applications.
The SKP2 protein is a component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which plays a crucial role in the regulation of the cell cycle. SKP2 promotes cell cycle progression by facilitating the degradation of cyclin-dependent kinase (CDK) inhibitors and promoting the activation of CDKs, which drive the cell cycle forward. SKP2 has also been shown to play a role in the regulation of DNA replication and repair, as well as apoptosis. Dysregulation of SKP2 has been linked to various human diseases, including cancer, making it an important target for therapeutic intervention.
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