Code | CSB-RA224072A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The production of the SKP2 recombinant monoclonal antibody is a complex and precise process. The initial steps involve harvesting the SKP2 monoclonal antibody and sequencing its gene. And then, constructing a vector carrying the SKP2 monoclonal antibody gene and transferring it into a host cell line for culturing. To synthesize the SKP2 monoclonal antibody, a synthesized peptide from human SKP2 is used as an immunogen. The SKP2 recombinant monoclonal antibody is purified using affinity chromatography to ensure high purity and specificity. Its specificity is then confirmed through ELISA, IHC, and IF assays to verify that it correctly recognizes its target. It only reacts with the human SKP2 protein.
SKP2, the component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, is a critical regulator of the cell cycle and DNA damage response. SKP2 plays a critical role in cell cycle progression by promoting the degradation of specific cell cycle inhibitors, such as p27Kip1 and p21Cip1, enhancing CDK activity and cell cycle progression. SKP2-mediated degradation of p53 can lead to the accumulation of DNA damage and genomic instability. It is frequently overexpressed in various types of cancer, including breast cancer, prostate cancer, and lung cancer. SKP2 overexpression can promote cell proliferation, survival, and invasion by degrading tumor suppressor proteins and promoting oncogenic signaling pathways.
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