Product Details

Uniprot No.

Target Names

Alternative Names

Excitatory amino acid transporter 2 (Glutamate/aspartate transporter II) (Sodium-dependent glutamate/aspartate transporter 2) (Solute carrier family 1 member 2), SLC1A2, EAAT2 GLT1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human SLC1A2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

28F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SLC1A2, also known as EAAT2 or GLT-1, serves as the predominant glutamate transporter in the central nervous system, responsible for clearing the majority of extracellular glutamate following synaptic transmission. This critical function protects neurons from excitotoxic damage, making SLC1A2 a key focus in neurodegenerative disease research, including studies of amyotrophic lateral sclerosis, Alzheimer's disease, and stroke pathology. Dysregulation of this transporter has been implicated in disrupted glutamatergic signaling and neuronal vulnerability across multiple disease states.

This recombinant monoclonal antibody, clone 28F9, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs in a controlled recombinant system, you can expect consistent performance across lots, eliminating the variability that often complicates experiments using traditional hybridoma-derived antibodies.

Validation studies demonstrate reliable detection of human SLC1A2 across multiple experimental platforms. Western blot analysis of U251 glioblastoma cell lysate reveals a clean band at the predicted molecular weight of 62 kDa, confirming specificity for the target protein. Flow cytometry validation using MCF-7 cells shows clear separation between antibody-stained and control populations, indicating suitability for surface expression studies. These validated applications, along with ELISA compatibility, provide flexibility whether you are quantifying expression levels, analyzing protein in cell lysates, or characterizing transporter localization on intact cells.

This antibody supports investigations spanning neuroscience, metabolism, and signal transduction research, offering a dependable tool for researchers exploring glutamate homeostasis and its implications in both physiological and pathological contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate.

Gene References into Functions

Therefore these activated forms of astrocytes with increased GLT-1 expression may exert beneficial roles in preserving cognitive function, even in the presence of amyloid-beta and neurofibrillary tangles in Alzheimer disease. PMID: 29374250
that rs3794087 of SLC1A2 does not lead to an increased risk of PD in the Chinese Han population PMID: 29275184
results suggest that the TM4 domain of GLT-1, and potentially other glutamate transporters, undergoes a complex conformational shift during substrate translocation, which involves an increase in the proximity of the TM2 and TM4 domains in the inward-facing conformation. PMID: 27698371
Authors demonstrated that the upregulation of GLT1 corrected Purkinje cell firing and motor incoordination in myotonic dystrophy. PMID: 28658620
This study demonstrated that EAAT2 expression is enhanced in the ET dentate nucleus, in contrast to differentially reduced EAAT2 levels in the ET cerebellar cortex. PMID: 27624392
we summarize the history leading up to the recognition of GLT-1a as a presynaptic glutamate transporter--{REVIEW} PMID: 27129805
This study provides further evidence for SLC1A2 mutations in epileptic encephalopathies and suggests a gain-of-function mechanism for this rather severe presentation. PMID: 28777935
GLT1 was demonstrated by luciferase assay to be a target of miR-31-5p and miR-200c-3p, and both its mRNA and protein (immunohistochemistry) significantly decreased with age in liver biopsies and in hepatic centrilobular zone, respectively PMID: 27995756
Mutations in SLC1A2 and CACNA1A Are Important Causes of Epileptic Encephalopathies PMID: 27476654
The results of this study suggested SLC1A2 rs3794087 may decrease the risk for Parkinson's disease in a Chinese cohort, but do not support a role in the susceptibility to amyotrophic lateral sclerosis or multiple system atrophy. PMID: 27206883
that Abeta1-42 oligomers could cause disturbances in insulin/Akt/EAAT signaling in astrocytes PMID: 26358886
This study showed a lack of association between of SLC1A2 rs3794087 with the risk for essential tremor. PMID: 27456607
Results suggest that genetic variation (rs4354668 and its haplotypes) in SLC1A2 may be involved in impaired executive function, which adds to the current body of knowledge regarding the risk of schizophrenia and the impairment of cognitive performance PMID: 26459047
SPAK and OSR1 are powerful negative regulators of the excitatory glutamate transporters EAAT1 and EAAT2. PMID: 26233565
The results of the meta-analysis suggest that rs3794087 is not associated with the risk for essential tremor. Systematic review and meta-analysis. PMID: 26313486
PPARgamma agonist pioglitazone has a role in modulating EAAT2 expression in glioma cells PMID: 26046374
Two recurrent SLC1A2 missense variants and one recurrent 5'-untranslated region variant were found to be associated with susceptibility to the development of bipolar disorder and schizophrenia. PMID: 25406999
The Hydroxyl Side Chain of a Highly Conserved Serine Residue Is Required for Cation Selectivity and Substrate Transport in the Glial Glutamate Transporter GLT-1/SLC1A2. PMID: 26483543
Splice variant EAAT2b levels are increased in populations of anterior cingulate cortex pyramidal cells in schizophrenic patients. PMID: 26057049
transcriptional factor yin yang 1 plays a critical role in the repressive effects of various neurotoxins, such as manganese (Mn), on EAAT2 expression. In this review, we will focus on transcriptional epigenetics and translational regulation of EAAT2 PMID: 25064045
Results showed that EAAT2 levels were significantly decreased in the essential tremor cerebellar cortex, in contrast to similar levels of EAAT1 levels between essential tremor cases and controls PMID: 25391854
the delivered miR-124 increased the expression of the glutamate transporters, EAAT1 in NPCs and EAAT2 in both NPCs and astrocytes. PMID: 25036385
Results describe EAAT1 and EAAT2 labeling in the prefrontal cortex in human postmortem control brains at the light and electron microscopic level PMID: 25064059
EAAT-2 glutamate transporter has a role in human liver cholestasis PMID: 24587631
results statistically show that a reduction in GAD1 and SCL1A2 expression in the dorsolateral prefrontal cortex in subjects with major depressive disorder is related to a possible attenuated RAF/MEK/ERK pathway PMID: 24652383
sumoylated EAAT2 localizes to intracellular compartments, whereas non-sumoylated EAAT2 resides on the plasma membrane PMID: 24753081
This study demonstrated an association between glutamate transporter polymorphism and early stress in influencing hippocampal gray matter volume in these patients. PMID: 24518437
The rs3794087 genotype and allelic variants were not related with the risk for migraine in Caucasian Spanish people. PMID: 24412224
SNP rs3794087 is not related to risk for restless legs syndrome PMID: 24424098
IL-1beta treatment of AEG-1-overexpressing astrocytes significantly lowered expression of excitatory amino acid transporter 2 PMID: 24855648
It does not play a role in susceptibility to essential tremor. PMID: 24139280
findings suggest important roles for up-regulated EAAT2 in chronic brain ischemia especially in the regulation of high-affinity of extracellular glutamate and minimization of white matter damage PMID: 23602887
The results showed that single nucleotide polymorphism rs3794087 was associated with essential tremor among the Taiwanese. PMID: 23951268
study suggests that SLC1A2 rs3794087 is not associated with the risk for developing familial essential tremor in the Spanish population PMID: 23949322
This study demonistrated that association between polymorphisms in the glutamate transporter SLC1A2 and Parkinson's disease. PMID: 23390085
The results of this study showed that the expression of The membrane transporters SLC1A2 and SLC1A3 was diminished in the major depressive disorder group compared to controls. PMID: 23706640
It transports neurotoxic glutamic acid, which causes major mental illnesses.(review) PMID: 24334928
Major depressive disorder is associated with unhealthy astrocytes in the noradrenergic Locus Coeruleus, characterized here by a reduction in astrocyte glutamate transporter expression. PMID: 23415275
Decreased EAAT2 protein expression alters glutamate buffering and reuptake in superior temporal gyrus and hippocampus in patients with schizophrenia. PMID: 23356950
We provided confirmation of an association of SLC1A2 intronic variant (rs3794087) with Essential tremor in the Chinese population. PMID: 23596072
The expression of EAAT2 in pyramidal neurons during human brain development may contribute to cortical vulnerability to excitotoxicity during the critical period for perinatal hypoxic-ischemic encephalopathy PMID: 22522966
Increased EAAT2 expression can protect against status epilepticus-induced death, neuropathological changes, and chronic seizure development. PMID: 22513140
In lithium-untreated biopolar patients we found a significant effect of genotype on the total episode recurrence rate. PMID: 23023733
The presence of the G allele is associated, among patients with schizophrenia, with a disadvantageous effect on core cognitive functions that depend on prefrontal cortex activity. PMID: 22728822
an association between EAAT2 protein expression in the human nucleus accumbens and a genetic polymorphism of EAAT2. PMID: 22750157
Decreased SLC1A2 is associated with sporadic amyotrophic lateral sclerosis. PMID: 22903028
We identified SLC1A2 encoding the major glial high-affinity glutamate reuptake transporter in the brain as a potential essential tremor susceptibility gene PMID: 22764253
GLT-1 endocytosis is independent of its phosphorylation and that Nedd4-2 mediates PKC-dependent down-regulation of the transporter. PMID: 22505712
RNA editing in pre-mRNA EAAT2 appears to activate a cryptic alternative polyadenylation site, generating retention transcripts at a novel site in intron 7 of EAAT2. PMID: 21569822
Evidence against cellular internalization in vivo of NMO-IgG, aquaporin-4, and excitatory amino acid transporter 2 in neuromyelitis optica. PMID: 22069320

Hide All

Involvement in disease

Epileptic encephalopathy, early infantile, 41 (EIEE41)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A2 subfamily

Database Links

HGNC: 10940

OMIM: 600300

KEGG: hsa:6506

STRING: 9606.ENSP00000278379

UniGene: Hs.502338

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Code	CSB-RA051685A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: U251 whole cell lysate All lanes: EAAT2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa Overlay Peak curve showing MCF-7 cells surface stained with CSB-RA051685A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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SLC1A2 Recombinant Monoclonal Antibody

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Target Background