Product Details

Uniprot No.

P42226

Target Names

STAT6

Alternative Names

Signal transducer and activator of transcription 6 (IL-4 Stat), STAT6

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human STAT6

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

STAT6 serves as a critical transcription factor in the IL-4 and IL-13 signaling pathways, orchestrating immune responses, Th2 cell differentiation, and allergic inflammation. Beyond its immunological roles, STAT6 has emerged as a significant player in cancer biology, where its dysregulation contributes to tumor progression and therapeutic resistance across multiple malignancies. For researchers investigating these pathways, reliable detection of STAT6 is essential for understanding both normal cellular signaling and disease mechanisms.

This recombinant monoclonal antibody, clone 2F10, offers the reproducibility that demanding experiments require. Because it is produced from a defined sequence rather than traditional hybridoma methods, you can expect consistent performance across different lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format provides excellent sensitivity while the affinity-chromatography purification ensures minimal background interference.

Validation studies demonstrate robust performance across multiple experimental platforms. In western blot applications, this antibody reliably detects STAT6 in human cell lines including HeLa, 293, HepG2, and K562, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. The observed band at approximately 100 kDa runs slightly higher than the predicted molecular weights, which likely reflects post-translational modifications such as glycosylation or phosphorylation that are characteristic of active STAT6 signaling. For immunohistochemistry, validation in paraffin-embedded human kidney tissue demonstrates clear staining with standard antigen retrieval protocols.

Whether you are mapping IL-4 signaling dynamics, characterizing tumor microenvironments, or investigating epigenetic regulation, this antibody provides the flexibility to move seamlessly between western blot and tissue-based analyses within your research program.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA988543A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: STAT6 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 95, 75, 82 kDa Observed band size: 100 kDa IHC image of CSB-RA988543A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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STAT6 Recombinant Monoclonal Antibody

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