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TRAF2 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA786226A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate
    All lanes: TRAF2 antibody at 1:1500
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56, 62, 55, 54 kDa
    Observed band size: 56 kDa
  • IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA786226A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing Hela cells stained with CSB-RA786226A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • Immunoprecipitating TRAF2 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA786226A0HU in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA786226A0HU(2µg)+ Hela whole cell lysate(500µg)
    Lane 3: Hela whole cell lysate (10µg)
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Product Details

Uniprot No.
Q12933
Target Names
TRAF2
Alternative Names
TNF receptor-associated factor 2 (EC 2.3.2.27) (E3 ubiquitin-protein ligase TRAF2) (RING-type E3 ubiquitin transferase TRAF2) (Tumor necrosis factor type 2 receptor-associated protein 3), TRAF2, TRAP3
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human TRAF2
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9A5
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
FC 1:20-1:200
IP 1:200-1:1000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

TRAF2 serves as a critical signaling adaptor in the TNF receptor superfamily pathway, functioning as an E3 ubiquitin-protein ligase that orchestrates cellular responses ranging from NF-κB activation to apoptotic regulation. Its central role in inflammatory signaling and cell survival decisions makes it a compelling target for researchers investigating cancer biology, cardiovascular disease, and signal transduction mechanisms.

This recombinant monoclonal antibody, clone 9A5, offers the reproducibility that demanding experimental workflows require. Because it derives from a defined sequence rather than traditional hybridoma methods, you can expect consistent performance across lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics across diverse detection platforms.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms reliable TRAF2 detection at the expected 56 kDa molecular weight across a broad panel of human cell lines including HepG2, HeLa, 293T, Jurkat, Raji, and K562, giving you confidence when working with various cellular models. Immunohistochemistry staining has been validated in both normal human kidney tissue and cervical cancer specimens, supporting tissue-based investigations. Flow cytometry analysis in HeLa cells shows clear population separation from isotype controls, enabling quantitative single-cell studies. Immunoprecipitation capability has been confirmed in HeLa lysates, facilitating protein interaction and complex isolation experiments.

Whether you're mapping TNF receptor signaling networks, investigating ubiquitination-dependent regulatory mechanisms, or exploring TRAF2's contributions to disease pathology, this antibody provides the application flexibility and batch-to-batch reliability essential for generating publication-quality data.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

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Target Background

Function
Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Gene References into Functions
  1. The TRAIP coiled-coil domain altered its stoichiometry between dimer and trimer in a concentration-dependent manner. Additionally, the TRAIP RING domain induced even higher-ordered assembly, which was necessary for interacting with the TRAF-N domain of TRAF2 but not TRAF1. PMID: 30127245
  2. high levels of CASC9.5 expression promote the proliferation, metastasis and metabolism of lung adenocarcinoma cells and might serve as a prognostic indicator. PMID: 29311633
  3. down-regulation of USP48 increases E-cadherin expression and epithelial barrier integrity through reducing TRAF2 stability PMID: 28874458
  4. DR5-Cbl-b/c-Cbl-TRAF2 complex inhibited TRAIL-induced apoptosis by promoting TRAF2-mediated polyubiquitination of caspase-8 in gastric cancer cells. PMID: 28972304
  5. CPNE1 overexpression can upregulate TRAF2 expression in prostate cancer DU-145 cells as determined by Western blotting and immunofluorescence assays. PMID: 29151113
  6. TRAF2 functions as an important modulator in tumor metastasis and tumor microenvironment formation and is a novel independent prognostic factor of gastric cancer. PMID: 28482378
  7. High expression of TRAF2 can predict poorer prognosis of GBM and was identified as an independent biomarker in GBM prognosis. PMID: 28926524
  8. TRAF2 can influence in vitro growth of TRAIL-treated DU-145 cells at least partially via regulating SIRT1 expression. PMID: 28667915
  9. Intestinal I/R induced the membrane translocation and phosphorylation of PKCzeta. Pretreatment with the PKCzeta activator phosphatidylcholine remarkably attenuated gut injury by suppressing apoptosis. H/R induced PKCzeta to combine with TRAF2, which was phosphorylated by PKCzeta at Ser(55), but not at Ser(11), under intestinal I/R or H/R conditions PMID: 28726782
  10. Both S1P and caspase-8 are critical for TRAF2 stabilization, polyubiquitination, subsequent activation of JNK/AP1 signaling and MMP1 expression and final promotion of cell invasion. PMID: 28482915
  11. Results showed that high expression of TRAF2 was significantly associated with tumor stage of prostate cancer. PMID: 28855498
  12. The addition of nanomolar concentration of TRAF2 in GUVs also seems to exert a mechanical action. PMID: 28499815
  13. TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling PMID: 28489822
  14. destabilization of TRAF2 by miR-17 reduced the ability of TRAF2 to associate with cIAP2, resulting in the downregulation of TNF-alpha-induced NF-kappaBp65, c-Jun, and STAT3 nuclear translocation and the production of IL-6, IL-8, MMP-1, and MMP-13 in human rheumatoid arthritis synovial fibroblasts. PMID: 27534557
  15. HOXA1-mediated activation of NF-kappaB is non-transcriptional and the RBCK1 and TRAF2 influences on NF-kappaB are epistatic to HOXA1 PMID: 27382069
  16. RIPK1 collaborates with TRAF2 to inhibit murine and human hepatocarcinogenesis. PMID: 28017612
  17. Study presents the characterization of the peptide binding preferences of TRAFs 2, 3, and 5 using deep mutational scanning. The three TRAF proteins demonstrated different preferences for binding to members of the CD40 library, and three peptides from that library individually showed striking differences in affinity for the three TRAFs. PMID: 26779844
  18. TRAF2 expression was increased in gastric cancer patients as a result of DNA hypomethylation. PMID: 26823737
  19. Data show that when death receptor 5 (DR5) is suppressed, caspase-8 may recruit and stabilize TNF receptor-associated factor 2 (TRAF2) to form a metastasis and invasion signaling complex, resulting in activation of ERK signaling. PMID: 26510914
  20. these findings reveal a novel mechanism by which TRAF2 mediates Lys63-linked ubiquitination of DUSP14, leading to DUSP14 activation in T cells PMID: 26521044
  21. There was a significant positive correlation between TRAF2 and TRAF4 expression levels in malignant pleural effusion cells PMID: 26331901
  22. Data show that ectopic expression of interferon regulatory factor 1 (IRF-1) reduces NF-kappa B activity and suppresses TNF receptor-associated factor 2 (TRAF2) and inhibitor of apoptosis 1 protein (cIAP1) expression in breast cancer cells. PMID: 26011589
  23. The results identify TRAF2 as an important biological suppressor of necroptosis in vitro and in vivo. PMID: 25882049
  24. MAVS50, exposing a degenerate TRAF-binding motif within its N-terminus, effectively competed with full-length MAVS for recruiting TRAF2 and TRAF6 PMID: 26221961
  25. Presented is the trimer-to-monomer equilibrium transition of the TRAF2 C-terminal domain using both chemical (dilution/guanidinium hydrochloride) and mechanical stress (high pressure) to induce the dissociation of the native protein into subunits. PMID: 26390021
  26. Knockdown of IRE1alpha by siRNA dramatically abrogated CXC195-induced activation of TRAF2, ASK and JNK, formation of an IRE1alpha-TRAF2-ASK1 complex and caspase- and mitochondrial-dependent apoptosis in T24 cells PMID: 25797626
  27. PP4R1 could mediate the dephosphorylation of TRAF2 Ser11. PMID: 25134449
  28. TRAF2 acts as an antagonist of TRAIL- and CD95L-induced necroptosis. PMID: 25299769
  29. Data indicate that TRAF interacting protein TRIP negatively regulates the TNFR-associated factor 2 (TRAF2) ubiquitin-dependent pathway by modulating the TRAF2-sphingosine 1-phosphate (S1P) interaction. PMID: 25716317
  30. The phosphorylation of TRAF2 at serine 11 is essential for the survival of cancer cells harboring TRAF2 amplification. Observations identify TRAF2 as a frequently amplified oncogene. PMID: 24362534
  31. preventing CD40-TRAF2,3 or CD40-TRAF6 interaction inhibits pro-inflammatory responses in human non-haematopoietic cells. PMID: 25051892
  32. During stress-induced mitochondrial hyperfusion, MULAN forms a complex with TRAF2 and modulates its ubiquitylation, signifying that TRAF2 may serve as an ubiquitylated transmitter of NF-kappaB signaling in this pathway PMID: 24841215
  33. GSTP1-1's TRAF2-binding region involves the GSH-binding site. PMID: 24457959
  34. RNASET2 contributes to vitiligo pathogenesis by inhibiting TRAF2 expression. PMID: 24457966
  35. Data indicate that in monocyte- derived macrophages (MDMs) acutely infected with HIV-1 and treated with HCV rCore and HIV-1 rNef, the HIV-1 replication depends on an upstream signal mediated through TRAF2, TRAF5 and TRAF6. PMID: 23774506
  36. TRAF2 is a proviral factor for vaccinia virus that plays a role in promoting efficient viral entry, most likely via the plasma membrane. PMID: 24429366
  37. IpaH0722 dampens the acute inflammatory response by preferentially inhibiting the PKC-mediated activation of NF-kappaB by ubiquitinating TRAF2, a molecule downstream of PKC, and by promoting its proteasome-dependent degradation. PMID: 23754945
  38. it is hypothesized that ZC3H15 may interact with TRAF-2 functionally within the NF-kappaB pathway, and may be explored as a potential target in acute myeloid leukemia PMID: 23624947
  39. The results of this study showed TRAF2 is upregulated in relapsing-remitting multiple sclerosis. PMID: 23595117
  40. TRAF2 can predict relapse of ulcerative colitis PMID: 23638224
  41. Data indicate that the Src homology 3 domain-containing GEF (SGEF) promotes fibroblast growth factor-inducible 14 (Fn14) proinvasive signaling in glioblastoma via TNF receptor-associated factor 2 (TRAF2). PMID: 23775076
  42. Authors found that rotavirus Non-Structural Protein-1 (NSP1) interacts with TRAF2 and degrades it in a proteasome dependent manner. PMID: 23890813
  43. activation of TRAF-1 may be early events in the pathogenesis of inflammatory bowel disease PMID: 23414308
  44. Data iindicate that TRAF2 affects the localization and function of TRAF4 in breast cancer cell lines. PMID: 23743189
  45. TRAF2/NIK/NF-kappaB2 pathway regulates pancreatic ductal adenocarcinoma cell tumorigenicity PMID: 23301098
  46. Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6. PMID: 23608757
  47. RAD23A down-regulated TRAF2 protein level through ubiquitin-proteasome system. PMID: 23357418
  48. These results reveal a critical role for the MAPK8 activation pathway through TRAF2 and RIPK1 for TNFSF10-induced autophagy that blunts apoptosis in cancer cells. PMID: 23051914
  49. Changes in expression levels of TNFSF4 and TRAF2 mRNAs may significantly correlate with the pathogenesis of SLE, the disease activity and different clinical features of lupus, particularly lupus nephritis, atherosclerosis and arthritis PMID: 22847298
  50. A novel radioresistance mechanism through functional orchestration of rpS3, TRAF2, and NF-kappaB in non-small cell lung cancer cells, is reported. PMID: 23188828

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Subcellular Location
Cytoplasm.
Protein Families
TNF receptor-associated factor family, A subfamily
Database Links

HGNC: 12032

OMIM: 601895

KEGG: hsa:7186

STRING: 9606.ENSP00000247668

UniGene: Hs.522506

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Related pathways
MAPK signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Protein processing in endoplasmic reticulum IL-17 signaling pathway NOD-like receptor signaling pathway Osteoclast differentiation Adipocytokine signaling pathway ER Stress pathway
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