TUBB Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA025318MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in:A431 whole cell lysate (20µg), SH-SY5Y whole cell lysate (20µg), U251 whole cell lysate (20µg), 293T whole cell lysate (20µg), HepG2 whole cell lysate (20µg), K562 whole cell lysate (20µg), MCF7 whole cell lysate (20µg), NIH/3T3 whole cell lysate (20µg) All lanes: TUBB antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size:49 kDa Observed band size:55 kDa Exposure time:5s IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA025318MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L). Overlay Peak curve showing K562 cells stained with CSB-RA025318MA1HU (red line) at 1:100.The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P07437

Target Names

TUBB

Alternative Names

Beta 4 tubulin antibody; Beta 5 tubulin antibody; beta Ib tubulin antibody; Beta1 tubulin antibody; Class I beta tubulin antibody; M40 antibody; MGC117247 antibody; MGC16435 antibody; OK/SW cl.56 antibody; OK/SWcl.56 antibody; TBB5_HUMAN antibody; TUBB 1 antibody; TUBB 2 antibody; TUBB 5 antibody; TUBB antibody; TUBB1 antibody; TUBB2 antibody; TUBB5 antibody; tubulin beta 1 chain antibody; Tubulin beta 2 chain antibody; tubulin beta 5 chain antibody; Tubulin beta chain antibody; Tubulin beta class I antibody; tubulin beta polypeptide antibody; Tubulin beta-5 chain antibody

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human TUBB protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

16E11D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Beta-tubulin (TUBB) serves as a fundamental building block of the cytoskeleton, forming heterodimers with alpha-tubulin to assemble microtubules essential for cell division, intracellular transport, and maintaining cellular architecture. As a ubiquitously expressed protein, TUBB has become an indispensable loading control in protein expression studies while also serving as a valuable marker for investigating cytoskeletal dynamics, mitotic processes, and the mechanisms underlying microtubule-targeting chemotherapeutics.

This recombinant monoclonal antibody, clone 16E11D4, offers the reproducibility that demanding research requires. Generated against recombinant human TUBB protein and produced through recombinant technology, it delivers sequence-defined consistency between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms robust detection across a diverse panel of human cell lines including A431, SH-SY5Y, U251, 293T, HepG2, K562, and MCF7, as well as the mouse NIH/3T3 line, supporting cross-species reactivity for comparative studies. The observed band at approximately 55 kDa runs slightly higher than the predicted 49 kDa molecular weight, a shift commonly attributed to post-translational modifications including tubulin-specific acetylation and polyglutamylation. Immunohistochemistry validation in paraffin-embedded human tonsil, brain, and breast cancer tissues demonstrates reliable performance in tissue-based applications, while immunofluorescence staining in HeLa cells and flow cytometry analysis in K562 cells extend its utility to single-cell resolution studies.

Whether establishing loading controls, investigating cytoskeletal organization, or exploring signal transduction pathways, this antibody provides the technical foundation for reliable, reproducible results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Gene References into Functions

human tumor cells can acquire spontaneous mutations in beta1-tubulin that cause resistance to paclitaxel PMID: 20103599
Roles of tubulin beta 1,3 residues Ala428 and Thr429 in microtubule formation in vivo. PMID: 19074767
TUBB exon 4 mutations and mismatch repair defects do not play a significant role in paclitaxel/cisplatin resistance PMID: 16095531
Mutations in either TUBB or MAPRE2 cause circumferential skin creases Kunze type. PMID: 26637975
the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
Leucine point mutations viz. L215H, L217R, and L225M were reported for paclitaxel resistance in various cancers. In the current study, molecular mechanism of these resistance causing mutations in TUBB1 was explored using molecular docking, molecular dynamics simulation, binding energy estimation, free energy decomposition, principle component analysis and free energy landscape methods. PMID: 27233604
Data suggest a looser binding of the ligand in tubulin mutants. PMID: 26081685
Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
Data suggest that, while lacking a stable structure, NFL-TBS.40-63 peptide (a peptide derived from light neurofilament protein) preferentially binds on a specific single site located near C-terminal end of beta-tubulin. PMID: 26016807
Citrullination of TUBB is associated with neoplasms. PMID: 24099319
mechanism of binding and stabilization of microtubules in mammalian cells can be effectively modeled in yeast and also having the advantage of lacking any beta-tubulin isotypes that can complicate interpretation of experiments in mammalian cells. PMID: 24161989
Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
results provide insight into the functional repertoire of the tubulin gene family, specifically implicating TUBB5 in embryonic neurogenesis and microcephaly PMID: 23246003
TQ induced a concentration- and time-dependent degradation of alpha/beta tubulin in both cancer cell types. PMID: 21881916
Allele frequencies between a group of 191 ITP patients & controls showed no direct aetiological role for SNP (R307H), but it was associated with immunomodulatory treatment failure. PMID: 23157319
Data show that knockdown of S100P led to downregulation of thioredoxin 1 and beta-tubulin and upregulation of RhoGDIA, all potential therapeutic targets in cancer. PMID: 21327297
This is the first cell-based evidence to support a beta-tubulin-binding site for peloruside A and laulimalide. PMID: 21653684
Mutational analysis of the class I beta-tubulin gene in human breast cancer PMID: 12209587
the influence of beta-tubulin mutations in paclitaxel resistance in advanced non-small cell lung cancer PMID: 12826311
Review of studies comparing beta-tubulin mutations with antitubulin drug resistance. Throws doubts on earlier such correlation because of the existence of many pseudogenes for beta-tubulin. PMID: 15003198
These data indicate that phosphorylation of tubulin by Cdk1 could be involved in the regulation of microtubule dynamics during mitosis. PMID: 16371510
cDNA subtraction revealed increased expression of alpha3-tubulin in the taxol-resistant cell line. PMID: 16380805
Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
Results suggest that glutamate198 in beta-tubulin is a critical determinant for microtubule stability and Taxol resistance. PMID: 17869412
This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953

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Involvement in disease

Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6); Skin creases, congenital symmetric circumferential, 1 (CSCSC1)

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Tubulin family

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Database Links

HGNC: 20778

OMIM: 156610

KEGG: hsa:203068

STRING: 9606.ENSP00000339001

UniGene: Hs.636480

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Gap junction Cell markers

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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