Code | CSB-RA977675A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The TXN recombinant monoclonal antibody is used for detecting human and rat TXN proteins in ELISA and WB applications. It is produced using recombinant DNA technology. The TXN monoclonal antibody gene is synthesized by sequencing the cDNA of the TXN antibody-producing hybridomas. The hybridomas are produced by fusing B cells isolated from animals immunized with a synthesized peptide derived from human TXN with myeloma cells. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. The resulting TXN recombinant monoclonal antibody is purified using affinity chromatography from the cell culture supernatant.
TXN acts as a disulfide reductase enzyme that can reduce disulfide bonds (-S-S-) within proteins and other molecules, thereby regulating their activity. TXN plays a critical role in redox signaling and cellular antioxidant defense. TXN can reduce oxidized cysteine residues in proteins, which can modify their activity or stability. TXN can also reduce hydrogen peroxide and other reactive oxygen species, thereby preventing oxidative damage to cells. It also regulates cellular processes, including cell proliferation and immune responses.
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