Code | CSB-RA010429A20me3HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
ICC | 1:50-1:300 |
The production of the tri-methyl-histone H4 (K20) recombinant monoclonal antibody starts with the isolation of genes responsible for encoding the HIST1H4A antibody from rabbits previously immunized with a synthesized peptide derived from the human HIST1H4A protein tri-methylated at K20. These antibody genes are then meticulously cloned into specialized expression vectors. Following this genetic modification, the modified vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. After this cultivation phase, the tri-methyl-histone H4 (K20) recombinant monoclonal antibody is subjected to a rigorous purification process utilizing affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a battery of tests, including ELISA, WB, and ICC tests, conclusively confirming its ability to interact effectively with the human HIST1H4A protein tri-methylated at K20.
Tri-methylation of HIST1H4A at K20 is an epigenetic modification associated with gene repression and the formation of repressive chromatin structures, such as heterochromatin and silenced gene loci. It plays a vital role in regulating gene expression, maintaining chromatin integrity, and contributing to genome stability.
There are currently no reviews for this product.