Code | CSB-RA276627A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The XRCC5 recombinant monoclonal antibody production uses protein technology and DNA recombinant technology. Mice are first immunized with a synthesized peptide derived from human XRCC5, and their spleen is later removed under aseptic conditions to extract total RNA. The cDNA obtained by RNA reverse transcription is used as a template for PCR amplification of the XRCC5 antibody gene. The XRCC5 antibody gene is then inserted into a vector and transfected into host cells for culture. Finally, the XRCC5 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography, which is verified for use in detecting human XRCC5 protein in ELISA, WB, IHC, and IF experiments.
The XRCC5 protein. also known as Ku80, is a DNA repair protein that plays a critical role in the non-homologous end joining (NHEJ) pathway that is involved in the repair of double-strand breaks (DSBs) in DNA. Specifically, XRCC5 plays a crucial role in the initial recognition and binding of the DSB, as well as in the regulation of DNA-PKcs activity. In addition to its role in DNA repair, XRCC5 has also been implicated in other cellular processes such as telomere maintenance, transcriptional regulation, and apoptosis.
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