What is a His Tag? A Guide from Definition to Applications


[1] Porath N, Ben-Shaul Y, Friedberg I. Proceedings: Some properties of membrane-bound adenosine triphosphatase of Euglena gracilis chloroplasts. Isr J Med Sci. 1975;11:1189–1190.

[2] Porath J, Carlsson J, et al. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation [J]. Nature 258: 598–599.

[3] Hochuli E, Dobeli H, Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr. 1987;411:177–184.

[4] Hochuli E. Large-scale chromatography of recombinant proteins. J Chromatogr. 1988;444:293–302.

[5] Riguero V, Clifford R, et al. Immobilized metal affinity chromatography optimization for poly-histidine tagged proteins [J]. J Chromatogr A. 2020 Oct 11;1629:461505.

[6] Panagiotidis CA, Silverstein SJ. pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins [J]. Gene. 1995 Oct 16;164(1):45-7.

[7] Kim JS, Raines RT. Peptide tags for a dual affinity fusion system [J]. Anal Biochem. 1994 May 15;219(1):165-6.

[8] Nilsson J, Larsson M, et al. Multiple affinity domains for the detection, purification and immobilization of recombinant proteins [J]. J Mol Recognit. 1996 Sep-Dec;9(5-6):585-94.

[9] Zentgraf H, Frey M, et al. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody [J]. Nucleic Acids Res. 1995;23:3347–3348.

Cite this article

CUSABIO team. What is a His Tag? A Guide from Definition to Applications. https://www.cusabio.com/c-21182.html


Leave a Comment

Your email address will not be published. Required fields are marked *
*CAPTCHA verification
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1