DOK3 Antibody

Code CSB-PA007019LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: Mouse heart tissue, Human placenta tissue
    All lanes: DOK3 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54, 24, 36, 25 kDa
    Observed band size: 54 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA007019LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA007019LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DOK3 Polyclonal antibody
Uniprot No.
Target Names
DOK3
Alternative Names
Docking protein 3 antibody; DOK 3 antibody; DOK Like Protein antibody; Dok3 antibody; DOK3_HUMAN antibody; DOKL Pending antibody; Downstream of tyrosine kinase 3 antibody; p62 DOK Like Protein antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Docking protein 3 protein (1-146AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DOK3 Antibody (Product code: CSB-PA007019LA01HU) is Non-conjugated. For DOK3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA007019LB01HU DOK3 Antibody, HRP conjugated ELISA
FITC CSB-PA007019LC01HU DOK3 Antibody, FITC conjugated
Biotin CSB-PA007019LD01HU DOK3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The DOK3 antibody is generated from rabbits immunized with a peptide corresponding to amino acids 1-146 of the recombinant DOK3 protein. It is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is over 95% using protein G purified. It can react with human and mouse samples. The target protein DOK3 is an adaptor molecule preferentially expressed in hematopoietic cells, which act as a cell type–specific regulator downstream of various immune receptors, including TLR3, TLR4, and B cell receptor (BCR), during viral infection, LPS stimulation as well as plasma cell differentiation. And DOK3 is dispensable for the development of immune cells. And this DOK3 antibody can be used to detect the DOK3 protein in ELISA, WB, IHC, and IF analyses.

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Target Background

Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.
Gene References into Functions
  1. Mutations in DOK3 gene is associated with prostate cancer PMID: 26585945
  2. DOK2 and DOK3 expression was significantly reduced in HTLV-1-infected T cells. PMID: 27265473
  3. The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters PMID: 23223229
  4. absence of DOK3 increases LPS signaling, contributing to LPS-induced tolerance. Thus, DOK3 plays a role in TLR signaling during both naive and endotoxin-induced tolerant conditions PMID: 22761938
  5. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
  6. The novel platelet adapter Dok-3 is tyrosine phosphorylated in an Src kinase-independent manner downstream of alphaIIbbeta3 in human platelets, leading to an interaction with Grb2 and SHIP-1. PMID: 19682241
  7. findings indicate that Dok-3 sequesters Grb2 from Shc and inhibits the Ras-Erk pathway downstream of PTKs PMID: 16436051

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
DOK family, Type A subfamily
Tissue Specificity
Expressed in spleen.
Database Links

HGNC: 24583

OMIM: 611435

KEGG: hsa:79930

STRING: 9606.ENSP00000349727

UniGene: Hs.720849

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