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Recombinant Human 5'-nucleotidase (NT5E), partial (Active)

Recombinant Human 5'-nucleotidase (NT5E), partial (Active)

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Code	CSB-AP005071HU
Abbreviation	Recombinant Human NT5E protein, partial (Active)
MSDS	MSDS Datasheet
Size	$290
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
Customer Reviews and Q&A

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD73-His at 2μg/ml can bind Anti-Human CD73 mAb, the ED50 of Anti-Human CD73 mAb is 3.33 ng/ml.

Target Names

NT5E

Uniprot No.

AAH65937.1

Research Area

Cardiovascular

Alternative Names

5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73); 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

27-547aa

Complete Sequence

WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADETFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

Mol. Weight

58.76 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder

Buffer

0.2 μm Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Datasheet & COA

Please contact us to get it.

Description

CD73 (NT5E) is a key ectoenzyme that hydrolyzes extracellular AMP to adenosine, modulating purinergic signaling in cardiovascular and immune microenvironments. This recombinant human construct spans residues 27–547, expressed in mammalian cells with a C-terminal 6xHis tag positioned to preserve the catalytic domain's accessibility for substrate engagement. Functional validation confirms binding activity via ELISA, where immobilized CD73-His at 2 μg/ml binds anti-human CD73 mAb with an ED50 of 3.33 ng/ml, supporting its use as a positive control in enzyme-linked assays, inhibitor screening for IC50 determination, and antibody characterization studies. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for accurate kinetic parameter analysis (Km, Vmax, kcat) and substrate specificity profiling where contaminant phosphatases would otherwise confound enzymatic readouts.