Recombinant Human C-C motif chemokine 3 protein(CCL3) (Active)

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Code CSB-AP000841HU
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Product Details

Purity >96% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Target Names CCL3
Uniprot No. P10147
Research Area Immunology
Alternative Names C C motif chemokine 3; CCL 3; CCL3; CCL3_HUMAN; Chemokine (C C motif) ligand 3; Chemokine C C motif ligand 3; Chemokine ligand 3; G0/G1 switch regulatory protein 19 1; G0/G1 switch regulatory protein 19-1; G0S19 1; G0S19 1 protein; Heparin binding chemotaxis protein; L2G25B ; LD78 alpha; LD78-alpha(4-69); LD78alpha; Macrophage inflammatory protein 1 alpha ; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein 1a; MIP 1 alpha; MIP 1A; MIP-1-alpha; MIP-1-alpha(4-69); MIP1 alpha; MIP1A; PAT 464.1; SCYA 3; SCYA3; SIS alpha; SIS beta; SIS-beta; Small inducible cytokine A3 ; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Species Homo sapiens (Human)
Source E.coli
Expression Region 23-92aa
Complete Sequence ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
Mol. Weight 7.8 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Gene References into Functions
  1. Tumor levels of CCL2 (mean +/- standard deviation = 530.1 +/- 613.9 pg/mg), CCL3 (102.7 +/- 106.0 pg/mg), and CCL4 (64.98 +/- 48.09 pg/mg) were higher than those found in healthy tissue PMID: 30419802
  2. During early remission from methamphetamine (MA), model mice exhibited increased anxiety-like behavior and reduced expression of chemokine (C-C motif) ligand 3 (ccl3) in the hippocampus relative to saline-treated mice. Human adults actively dependent on MA and those in early remission had elevated symptoms of anxiety as well as reductions in plasma levels of CCL3, relative to adults with no history of MA abuse. PMID: 29402784
  3. As depression became more severe the serum levels of MIP-1alpha increased. PMID: 29339257
  4. results suggested that genetic variants at the CCL3 and CCL4 loci may be marker SNPs for risk of HCV treatment outcome. PMID: 29705123
  5. Telomere length and mRNA expressions of IL6 and MIP1alpha were significantly longer or higher in bone marrow mesenchymal stem cells of multiple myeloma than those of controls. PMID: 28677723
  6. The CCL3 and CCL4 chemokines might not be involved in differential susceptibility to the digestive and cardiac clinical forms of chronic Chagas disease, and it seems they do not influence the development of LVSD. PMID: 28002786
  7. The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
  8. the DR3/TL1A pathway directly enhances human OC formation and resorptive activity, controlling expression and activation of CCL3 and MMP-9. PMID: 28062298
  9. serum levels of MIP-1alpha could predict survival outcomes in patients with ENKTL. PMID: 26928433
  10. elevated CCL3 in the leukemic environment suppresses erythropoiesis via CCR1-p38 activation PMID: 27109512
  11. analysis of sudden infant death syndrome brains shows downregulation of MyD88 in tissue from SIDS brains, as well as the downregulation of the genes encoding CCL3 and UNC13 in the liver PMID: 26959483
  12. we demonstrate an association of CCL3 expression by CLL cells with increased numbers of CD3+ T cells and CD57+ cells in the lymph node microenvironment, which may promote CLL cell survival and proliferation. PMID: 26458057
  13. The N termini of CC chemokines are shown to be involved in receptor binding and oligomerization. We also report an alternative CCL3 oligomer structure that reveals how conformational changes in CCL3 N termini profoundly alter its surface properties and dimer-dimer interactions to affect GAG binding and oligomerization. Such complexity in oligomerization and GAG binding enables intricate, physiologically relevant regulatio PMID: 27091995
  14. CCL3 promotes VEGF-A expression and angiogenesis in human osteosarcoma cells by down-regulating miR-374b expression via JNK, ERK, and p38 signaling pathways. PMID: 26713602
  15. The residues on the N-loop and beta-sheets of MIP-1a are close to both CCR1 and CCR5, and those in the C-terminal helix region are close to CCR5. PMID: 26472202
  16. An increased MIP-1alpha level in nasopharyngeal aspirates from patients with acute respiratory symptoms during the first wheezing episode caused by viral infections might predict recurrent wheezing. PMID: 26494023
  17. Tregs from subjects with established type 1 diabetes were impaired in their ability to produce CCL3 and CCL4. PMID: 26854929
  18. Pleural and serum MIP1a were lower in patients with lung cancer compared to lung metastasis, pneumonia, tuberculosis, and transudate pleural effusion. PMID: 26620310
  19. AEG-1 mediates CCL3/CCR5-induced EMT development via both Erk1/2 and Akt signaling pathway in CM patients, which indicates CCL3/CCR5-AEG-1-EMT pathway could be suggested as a useful target to affect the progression of CM. PMID: 26134542
  20. The EP in TKA differed from EP in aseptically failed THA by lower CCL3 and DC-STAMP mRNA and protein expression. EP of all studied inflammatory and osteoclastogenic molecules were similar in knee and hip OA. PMID: 25151085
  21. A significantly higher percentage of healthy asymptomatic persons possibly exposed to asbestos had detectable levels of serum CCL3 compared to healthy unexposed control subjects. PMID: 25940505
  22. APOE varepsilon4 genotype specifically modulates astrocyte secretion of potent microglial chemotactic agents, including CCL3 PMID: 25092803
  23. Macrophage inflammatory protein-1alpha shows predictive value as a risk marker for subjects and sites vulnerable to bone loss in a longitudinal model of aggressive periodontitis. PMID: 24901458
  24. In COPD patients, CCL3 is upregulated in sputum and may facilitate recruitment of macrophages into airways. PMID: 25183374
  25. Serum/urinary MIP-1a responds to infection but cannot be used as reliable biomarker in childhood urinary tract infections. PMID: 25618120
  26. Sequence variants in the CCL3 chemokine gene family in the HapMap West African reference population have been identified. PMID: 24952210
  27. MIP-1alpha levels in Jurkat cells appeared to be an important factor for its transendothelial migration PMID: 25245399
  28. se results indicate that CCL2 and CCL3 are associated with progression of oral squamous cell carcinoma PMID: 25060177
  29. Our results confirm the strong influence of previous immunity in subsequent dengue infections, and confer a possible pathogenic role to CCR1 and CCL3 in dengue disease and a possible protective role for CCL5, probably through CCR5 interaction. PMID: 24157267
  30. The detection of basal level of IgM rheumatoid factor, IgM and also certain cytokines can be useful in prognosis of effectiveness of rituximab therapy under rheumatoid arthritis. PMID: 25080789
  31. Gene expression profiling in bone marrow from multiple myeloma patients with and without osteolysis vs controls with other hematologic neoplasms showed only weak CCL3 expression. PMID: 24556596
  32. MIP-1alpha is a predominant factor responsible for the enhancement of bone resorption and increased angiogenesis PMID: 24277416
  33. Greater adiposity is associated with higher MIP-1alpha and lower soluble CD14 levels, possibly reflecting an important role for cells of the monocyte/macrophage lineage. PMID: 23748193
  34. Transcription of CCL3 and CCL4 by THP-1 monocytic cells up-regulated in the presence of 27-Hydroxycholesterol and 7alpha-hydroxycholesterol. PMID: 24370436
  35. the role of Tax2-mediated activation of the nuclear factor kappa B (NF-kappaB) signalling pathway on the production of the anti-viral CC-chemokines MIP-1alpha, MIP-1beta and RANTES. PMID: 24116893
  36. Data indicate that VIP and PACAP increased macrophage resistance to HIV-1 replication by inducing the synthesis of beta-chemokines CCL3 and CCL5 and IL-10 following preferential activation of the receptors VPAC2 and PAC1. PMID: 23818986
  37. JAK2 and STAT3 activation is not essential for CCL3, CCL5 or CCL8 induced chemotaxis. PMID: 22987449
  38. Epstein-Barr virus latent membrane protein 1 (LMP1)-mediated Jun N-terminal protein kinase activation was responsible for upregulation of CCL3 and CCL4 in B cells. PMID: 23760235
  39. either the CCL3 gene may play a significant role in CCL3 production and/or that as yet undefined mechanisms regulate production of CCL3 from variable CCL3L copy number. PMID: 23295355
  40. High serum CCL-3 is associated with disease severity of generalized pustular psoriasis. PMID: 23334651
  41. These findings suggest that MIP-1alpha and IL-17 may play important roles in acute exacerbation of asthma induced by RSV in an animal model. PMID: 23711864
  42. This study examined the role of three African CCL3 haplotypes in HIV-1 infant susceptibility, maternal HIV-1 transmissibility and HIV-1 disease progression in the mothers. PMID: 23151487
  43. These findings suggest that the increased expression of CCL3, CCR1, and CCR5 may influence the immune response in RAS by T(H)1 cytokine polarization. PMID: 22727097
  44. Our findings indicate that TNFalpha and IL-1beta modulate the expression of CCL3 in nucleus pulposis cells by controlling the activation of MAPK, NF-kappaB, and C/EBPbeta signaling. PMID: 23233369
  45. Our results suggest vital roles for CCL3-mTORC2-isoform PKB/Akt1 S473 phosphorylation axis in FOXP3+Treg cells and the development of psoriasis. PMID: 23223135
  46. Serum CCL3, CCL5 and CCL18 are independently associated with the risk of short-term mortality in acute coronary syndrome patients. PMID: 23029252
  47. Tumor-infiltrating monocytic myeloid-derived suppressor cells produce high levels of transgenic CCR5 ligands CCL3, CCL4, and CCL5 and directly attract regulatory T cells (Tregs) in a CCR5-dependent manner. PMID: 23152559
  48. MIP-1alpha was found to correlate with WHO grade among invasive gliomas, and MIP-1alpha promoted human glioblastoma cell proliferation and migration. PMID: 22307528
  49. The mRNA expression of MIP-1alpha was markedly higher in chronic nonbacterial prostatitis/chronic pelvic pain syndrome patients than in controls. PMID: 22295852
  50. Data indicate that IFN2b treatment upregulates the expression of CCR5, RANTES, MIP-1alpha and MIP-1beta on monocytes/macrophages. PMID: 22085486
  51. Patients possessing the compound decreased functional genotype of both CCL3L1 and CCR5 genes might be more likely to have recurrence of hepatitis B after transplantation. PMID: 22146622
  52. Data show that serum levels of the Dickkopf-related protein 1 (DKK1) and osteoclast-activating factor MIP-1alpha were significantly elevated in patients with monoclonal gammopathy of undetermined significance (MGUS). PMID: 22042700
  53. IL-27 priming results in enhanced lipopolysaccharide (LPS)-induced MIP-1alpha expression in human primary monocytes. PMID: 22156348
  54. Data suggest a role for MIP-1alpha in HZ-dependent enhancement of MMP-9 expression, release and activity observed in human monocytes, highlighting new detrimental effects of HZ-triggered proinflammatory response by phagocytic cells in falciparum malaria. PMID: 22118025
  55. The polymorphisms of MIP-1alpha -906 (TA)(6)/(TA)(6) and ApoE are independent genetic risk factors for ulcerative colitis. PMID: 21756796
  56. our data indicate that the interaction between TTP and CCL3 mRNA plays an important role in modulating localized inflammatory processes in tissues that are dissociated from the systemic manifestations of chronic inflammation. PMID: 21784977
  57. CCL3, in addition to its known catabolic activity, reduces bone formation by inhibiting osteoblast function, and therefore contributes to osteoblastosteoclast uncoupling in multiple myeloma. PMID: 21403648
  58. MIP-1alpha induces proliferation of nonasthmatic airway smooth muscle cells and decreases their rate of apoptosis. PMID: 21368236
  59. Data show that the ex vivo NKT cell response is predominantly comprised of the chemokines MIP1-alpha, and MIP1-beta as well as the Th1 cytokines IFN-gamma and TNF-alpha. PMID: 21082024
  60. Increased CCL3 expression is associated with disease progression in chronic lymphocytic leukemia. PMID: 21115978
  61. Crystal structures reveal that MIP-1 aggregation is a polymerization process and human MIP-1-alpha and MIP-1-beta form rod-shaped, double-helical polymers. PMID: 20959807
  62. In CD8+ T-lymphocyte transwell culture, MIP-1beta and MIP-1alpha strongly correlate with the magnitude of soluble noncytolytic suppression of CXCR4-tropic virus. PMID: 21030011
  63. CCL3 was associated with susceptibility to RA. A haplotype locating CCL3 on chromosome 17q12 had significant correlation with RA. The global similarity score of CCL3 was ranked in the top of all RA-related genes, excluding known susceptibility genes. PMID: 20518837
  64. CCAAT/Enhancer-binding protein {beta} and NF-{kappa}B mediate high level expression of chemokine genes CCL3 and CCL4 by human chondrocytes in response to IL-1{beta}. PMID: 20702408
  65. Data show that pre-CPB, plasma levels of EGF and MIP-1beta, 2 h following CPB, sVCAM-1, fractalkine and MIP-1ALPHA, and at later time points, sVCAM-1 and IL-6 were associated with AKI. PMID: 20482449
  66. CCL3 and CCL20-recruited dendritic cells modified by melanoma antigen gene-1 have a role in anti-tumor immunity against gastric cancer PMID: 20420712
  67. Data show that TLR activation was associated with significantly increased production of IL-1beta, IL-6, TNF-alpha, and CCL3. PMID: 20124101
  68. The structure of the complex of Evasin-1 and its high affinity ligand, CCL3, was solved. PMID: 20041127
  69. MIP-1 alpha promotes acute lung inflammation and injury in three murine models and is the key substrate in the lung for matrix metalloproteinase-8 during lipopolysaccharide-mediated murine acute lung injury. PMID: 20042585
  70. Inhibition of macrophage inflammatory protein-1 alpha production by Epstein-Barr virus. PMID: 11861262
  71. role in the development of osteolytic lesions in multiple myeloma PMID: 12200385
  72. Expression of MIP-1 alpha is induced by stimulation of monocytic cells through Fc gamma receptors and involves activation of CCAAT/enhancer-binding protein beta sites. PMID: 12218153
  73. Burned patients seem to be more susceptible to infectious complications when the production of MIP-1 alpha is impaired. PMID: 12370381
  74. role of IL-12 and IFN-gamma on inducing inflammatory chemokine (MIP-1alpha) secretion and down-regulating CCR5 expression in human T cells PMID: 12377943
  75. Lymphotactin, (MIP)-1alpha, and MIP-1beta chemokines were all rapidly induced by engagement of CD28 and were calcineurin sensitive. PMID: 12393716
  76. MIP-1alpha and MIP-1beta show diverging signaling capacities [review] PMID: 12401480
  77. may not only mark a subset of patients with a greater risk of having more severe disease but also may play a relevant pathophysiological role in human schistosomiasis mansoni PMID: 12447751
  78. Besides its role in development of osteolytic bone destruction, MIP-1 alpha also directly affects cell signaling pathways mediating growth, survival, and migration in MM cells and might play a pivotal role in the pathogenesis of MM. PMID: 12506012
  79. AML-1A and AML-1B regulate the expression of this protein in multiple myeloma cells. PMID: 12560229
  80. IL-1beta-induced MIP-1alpha and -1beta expression in NT2-N cells PMID: 12603824
  81. Cathepsin D specifically cleaves this protein that is expressed in human breast cancer. PMID: 12651610
  82. Potential interaction between CCR1 and its ligand, this protein is enduced by endogenously produced interleukin-1 in human hepatomas. PMID: 12651617
  83. Transcriptional activation of the MIP-1alpha promoter by RUNX1 and MOZ. PMID: 12771199
  84. Therefore, soluble factors such as prostaglandin E(2) released from lung fibroblasts are responsible for the co-culture-induced inhibition of macrophage-derived MIP-1alpha production. PMID: 14680814
  85. MIP-1-alpha is substantially regulated upon monocyte contact with various cell wall components from Gran-positive and Gram-negative bacteria. PMID: 14733721
  86. lymphocytes of HIV patients display a disrupted capacity to secrete the beta-chemokines MIP-1 alpha and MIP-1 beta, which may constitute a mechanism of immune dysfunction in progressive HIV infection PMID: 15585099
  87. produced by neonatal natural killer cells and contributes to suppession of HIV replication PMID: 15588341
  88. severe periodontal tissue destruction in Papillon-Lefevre syndrome may be related to excess accumulation of LD78beta and LD78alpha and dysregulation of the microbial-induced inflammatory response in the periodontium PMID: 15728180
  89. Involvement of the 3 10 helical-turn motif in chemokine function identifies a novel, functionally essential motif within chemokines. PMID: 16234357
  90. Higher amounts of MIP-1alpha, MIP-1beta, and RANTES were detected in plasma of HIV-1-positive individuals, particularly those with concomitant pulmonary tuberculosis, although the increase was not found to be statistically significant. PMID: 16379602
  91. HIV p17 was able to reduce MIP-1alpha secretion in IL-15 stimulated monocytes PMID: 16427155
  92. Levels of MIP-1alpha are low in plasma and follicular fluid of both menstrual and in vitro fertilization (IVF) cycles. PMID: 16439481
  93. High levels of CCL3 are present in synovial fluid of children with juvenile idiopathic arthritis. PMID: 16507178
  94. MIP-1 alpha promoter function, gene expression, and protein secretion were each down-regulated following inhibition of FGFR3 signaling. PMID: 16849642
  95. findings show that CCL3, CCL4 and CCL5 bind on the Leishmania promastigotes PMID: 17005260
  96. MIP-1alpha and other chemokines may constitute a link between the innate immune system and familial Mediterranean fever PMID: 17270460
  97. CCL3 concentration was much lower in CSF than in serum of the tickborne encephalitis patients, which argues against its significant role as chemoattractant in this condition. PMID: 17357337
  98. found a negative correlation between the tear levels of MIP-1alpha and clinical severity in Cystic fibrosis (CF) patients and a positive correlation between the tear levels of MIP-1alpha and the presence of dry eye findings in CF patients PMID: 17572013
  99. brain Alzheimer's disease-derived microvessels express high levels of MIP-1alpha mRNA and release high levels of MIP-1alpha protein PMID: 17656823
  100. Significantly higher expression of CCL3 is associated with tumor metastasis and local host defense in oral squamous cell carcinoma PMID: 17914560

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

HGNC: 10627

OMIM: 182283

KEGG: hsa:6348

STRING: 9606.ENSP00000225245

UniGene: Hs.514107

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