Bovine Interleukin 1β (IL- 1β) ELISA kit

Instructions
Code CSB-E12986B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 1, beta
Alternative Names IL1B ELISA Kit; Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
Abbreviation IL1B
Uniprot No. P09428
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %99
Range %95-108
1:2Average %94
Range %91-98
1:4Average %92
Range %88-95
1:8Average %87
Range %81-93
Recovery
The recovery of bovine IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-102
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.876 2.862 2.869 2.751
20002.444 2.439 2.442 2.324
10001.903 1.856 1.880 1.762
5001.412 1.403 1.408 1.290
2501.164 1.185 1.175 1.057
1250.809 0.799 0.804 0.686
62.50.471 0.479 0.475 0.357
00.117 0.118 0.118  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Gene References into Functions
  1. Mycobacterium bovis infected animals have a higher frequency of IFN-gamma producing CD4(+) T cells and elevated plasma IL-1beta levels. PMID: 28610788
  2. The presence of embryos increased endometrial IL1B protein locally, while no differences regarding IL1R1 protein and IL1B and IL1R1 mRNA were detected. PMID: 25955718
  3. For the inflammasome-dependent IL-1beta release, bovine monocytes require ATP in addition to a primary stimulus. This IL-1beta release depends on potassium efflux, but, in contrast to human and murine monocytes, does not require calcium influx or generation of reaction oxygen and is independent of the P2X7 receptor. PMID: 22728096
  4. Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease. PMID: 21722622
  5. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
  6. Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
  7. Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
  8. non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
  9. Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
  10. dynamic compression stimulates cell proliferation and proteoglycan synthesis in the presence of IL-1beta and/or inhibitors of the MAPKs and NFkappaB and AP-1 signalling pathways PMID: 18545934
  11. These results indicate that activation of the intrinsic antistaphylococcal response in bovine endothelial cells (BEC), enhanced by TNF-alpha and IL-1beta, is effective to eliminate S. aureus and S. epidermidis. PMID: 18625014
  12. the low friction of articular cartilage can be modified by TGF-beta1 and IL-1beta treatment and that the friction coefficient depends on multiple factors, including superficial zone protein localization and surface roughness PMID: 18683879
  13. These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361
  14. Data suggest an important regulatory mechanism of interleukin-1beta production, associated with microglial migration and proliferation, driven by ECM degradation and/or synthesis in an injured brain. PMID: 19250967

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Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Database Links

KEGG: bta:281251

STRING: 9913.ENSBTAP00000001745

UniGene: Bt.4856

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