Bovine adiponectin,ADP ELISA Kit

Code CSB-E14054B
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q3Y5Z3
Lead Time 3-5 working days
Abbreviation ADIPOQ
Target Name adiponectin, C1Q and collagen domain containing
Alias ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047|OTTHUMP00000210048|adipocyte, C1Q and collagen domain containing|adipocyte, C1Q and collagen domain-containing|adipo
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.125 μg/mL-50 μg/mL
Sensitivity 3.125 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-97
1:2Average %97
Range %93-104
1:4Average %85
Range %82-91
1:8Average %90
Range %85-97
Recovery
The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
500.212 0.207 0.210
250.345 0.332 0.339
12.50.589 0.607 0.598
6.251.077 1.106 1.092
3.1252.214 2.123 2.169
References
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Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW (By similarity).
Subcellular Location Secreted
Database Links

KEGG: bta:282865

STRING: 9913.ENSBTAP00000026395

UniGene: Bt.109637

Pathway PPAR signaling pathway
Adipocytokine signaling pathway
AMPK signaling

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