Bovine adiponectin,ADP ELISA Kit

Instructions
Code CSB-E14054B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name adiponectin, C1Q and collagen domain containing
Alternative Names ADIPOQ ELISA kit; ACRP30 ELISA kit; APM1Adiponectin ELISA kit; 30 kDa adipocyte complement-related protein ELISA kit; Adipocyte complement-related 30 kDa protein ELISA kit; ACRP30 ELISA kit; Adipocyte ELISA kit; C1q and collagen domain-containing protein ELISA kit; Adipose most abundant gene transcript 1 protein ELISA kit; apM-1 ELISA kit
Abbreviation ADIPOQ
Uniprot No. Q3Y5Z3
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.125 μg/mL-50 μg/mL
Sensitivity 3.125 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-97
1:2Average %97
Range %93-104
1:4Average %85
Range %82-91
1:8Average %90
Range %85-97
Recovery
The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
500.212 0.207 0.210
250.345 0.332 0.339
12.50.589 0.607 0.598
6.251.077 1.106 1.092
3.1252.214 2.123 2.169
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW (By similarity).
Gene References into Functions
  1. These data suggest that energy balance around parturition may regulate plasma adiponectin but do not support roles for lipid mobilization or sustained changes in the plasma concentration of leptin, insulin, growth hormone, or fatty acids. PMID: 28843695
  2. Low level expression of adiponectin mRNA was found in all areas of bovine mammary gland tissues examined. AdipoR1 and AdipoR2 mRNAs were also detected in mammary tissues and their expression was particularly prominent in the parenchyma and cistern. PMID: 25676879
  3. Data suggest that genetic variation in promoter region of ADIPOQ (SNPs g.81966235C>T, g.81966377T>C, and g.81966364D>I) contribute to adiposity/marbling in skeletal muscle/meat (and thus meat quality) of Hanwoo beef cattle of North Korea. PMID: 25817801
  4. These data suggest differential contribution of adipose tissue depots to circulating adiponectin. PMID: 24462180
  5. Association analysis indicated that variable duplication within the ADIPOQ gene is associated with body measurements. PMID: 24341634
  6. 14 polymorphisms of the ADIPOQ gene were observed in Chinese cattle; 2 polymorphisms PR_-135 A>G and PR_-68 G>C were located in the core promoter region of ADIPOQ; 3 haplotypes in the 2 polymorphic sites were detected which produce effects on ADIPOQ transcription and are associated with growth traits PMID: 24099391
  7. reduced plasma adiponectin belongs to the subset of hormonal adaptations in EL dairy cows facilitating mammary glucose uptake via promotion of insulin resistance PMID: 23077076
  8. The physiologic status of the ovary has significant effects on the natural expression patterns of adiponectin and its receptors in follicular and luteal cells of bovine ovary. PMID: 20047754
  9. The disulfide bonds help to maintain the mature octadecameric adiponectin structure and stabilize intermediates during the assembly. PMID: 19943704
  10. Gobular adiponectin increased NO production in aortic endothlium by increasing NO synthase activity. PMID: 14551684
  11. A 5bp deletion mutation within the bovine ACRP30 gene was firstly detected and confirmed in 991 cattle . PMID: 18446445
  12. results indicate decreasing adiponectin sensitivity in adipose tissue after calving which might be involved in the reduced insulin sensitivity of adipose tissue during lactation PMID: 19345551
  13. Adiponectin gene was proved to be closely related to carcass and meat quality traits (P < 0.05), which can be used as a candidate molecular marker for production of high-grade meat in Qinchuan beef cattle. PMID: 19840922

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Subcellular Location Secreted
Database Links

KEGG: bta:282865

STRING: 9913.ENSBTAP00000026395

UniGene: Bt.109637

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