Human Bcl-2-like protein 1(BCL2L1) ELISA kit

Instructions
Code CSB-EL002613HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name BCL2-like 1
Alternative Names BCL-XL/S, BCL2L, BCLX, Bcl-X, DKFZp781P2092, bcl-xL, bcl-xS, OTTHUMP00000030550
Abbreviation BCL2L1
Uniprot No. Q07817
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 88-95
1:2 Average % 95
Range % 90-100
1:4 Average % 97
Range % 94-103
1:8 Average % 101
Range % 96-105
Recovery
The recovery of human BCL2L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-94
EDTA plasma (n=4) 96 94-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.613 2.545 2.579 2.396
20 1.947 1.855 1.901 1.718
10 1.155 1.224 1.190 1.007
5 0.723 0.684 0.704 0.521
2.5 0.458 0.440 0.449 0.266
1.25 0.302 0.292 0.297 0.114
0.625 0.233 0.241 0.237 0.054
0 0.188 0.177 0.183  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.; FUNCTION
Subcellular Location Isoform Bcl-X(L): Mitochondrion inner membrane, Mitochondrion outer membrane, Mitochondrion matrix, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus membrane, Single-pass membrane protein, Cytoplasmic side
Protein Families Bcl-2 family
Tissue Specificity Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Database Links

HGNC: 992

OMIM: 600039

KEGG: hsa:598

STRING: 9606.ENSP00000302564

UniGene: Hs.516966

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