Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/mL can bind Anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC50 is 0.5355-1.248 ng/mL.

Target Names

ENPP3

Uniprot No.

O14638

Alternative Names

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; NPP3; Alkaline phosphodiesterase I ; Dinucleoside polyphosphatase ; Nucleotide diphosphatase; Nucleotide pyrophosphatase ; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; CD203c

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

48-875aa

Target Protein Sequence

LEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Mol. Weight

98.7 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

ENPP3 serves as a basophil activation marker (CD203c) and exhibits phosphodiesterase activity implicated in purinergic signaling pathways relevant to cancer biology. This mammalian-expressed construct spanning residues 48–875 carries an N-terminal 10xHis tag positioned outside the catalytic domain, preserving enzymatic function while enabling straightforward purification for kinetic studies. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 0.5355–1.248 ng/mL, confirming proper folding and epitope accessibility that supports use in inhibitor screening campaigns, IC50 determination assays, and as a positive control in enzyme-linked detection platforms. The protein meets the purity and endotoxin criteria typical for kinetic parameter analysis (Km, Vmax, kcat/Km) and substrate specificity profiling, with >95% purity by SDS-PAGE and <1.0 EU/μg endotoxin ensuring minimal interference in quantitative enzymatic activity measurements and drug candidate evaluation studies.

Target Background

Function

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.

Gene References into Functions

We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID: 29702283
Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID: 26585435
Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID: 23581640
Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID: 24497482
ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID: 23960081
The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID: 22722613
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID: 20975283
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID: 20159259
Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID: 20047266
Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID: 19418203
E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID: 14533006
E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID: 15072822
Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID: 15886225
In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Secreted.

Protein Families

Nucleotide pyrophosphatase/phosphodiesterase family

Tissue Specificity

Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.

Database Links

HGNC: 3358

OMIM: 602182

KEGG: hsa:5169

STRING: 9606.ENSP00000350265

UniGene: Hs.486489

Code	CSB-MP007681HUb0
Abbreviation	Recombinant Human ENPP3 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/ml can bind Anti-ENPP3 recombinant antibody (CSB-RA007681MA1HU), the EC₅₀ is 0.5355-1.248 ng/mL. Biological Activity Assay
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Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (ENPP3), partial (Active)

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