Product Details

Associated names

IF 1 ELISA Kit; IFG ELISA Kit; IFI ELISA Kit; IFN gamma ELISA Kit; IFN immune ELISA Kit; IFN, immune ELISA Kit; IFN-gamma ELISA Kit; IFNG ELISA Kit; IFNG_HUMAN ELISA Kit; Immune interferon ELISA Kit; Interferon gamma ELISA Kit; Type II Interferon ELISA Kit

Uniprot No.

P01579

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

7.8 pg/ml-500 pg/ml

Sensitivity

1.95 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	95
	Range %	92-99
1:4	Average %	91
	Range %	87-94
1:8	Average %	89
	Range %	86-93

Recovery

The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-96
EDTA plasma (n=4)	95	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

500

2.046

2.058

2.052

1.902

250

1.537

1.444

1.491

1.341

125

1.027

0.987

1.007

0.857

62.5

0.648

0.635

0.642

0.492

31.2

0.456

0.432

0.444

0.294

15.6

0.342

0.345

0.344

0.194

7.8

0.241

0.254

0.248

0.098

0.151

0.149

0.150

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of human interferon-gamma (IFN-γ), a pivotal type II interferon produced primarily by T cells and NK cells that orchestrates cell-mediated immune responses. Because IFN-γ levels can span from near-undetectable baseline concentrations in healthy plasma to sharply elevated values during active immune activation, the kit is engineered to capture this dynamic range across diverse experimental contexts.

Detection Range: The 7.8–500 pg/ml range aligns with the physiological and pathological concentrations of circulating IFN-γ, from low resting levels in healthy serum to elevated concentrations seen during infection, graft rejection, or Th1-driven inflammation. Mitogen-stimulated PBMC supernatants may require dilution to fall within range, while unstimulated samples can typically be assayed neat.

Sensitivity: At 1.95 pg/ml, the kit detects the low basal IFN-γ levels present in unstimulated plasma or early-phase immune responses, where concentrations often fall below 10 pg/ml.

Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants enables researchers to correlate systemic IFN-γ levels with local tissue production or ex vivo T cell and NK cell functional assays within a single study.

Assay Efficiency: The 1–5 hour protocol and 50–100 µl sample volume accommodate limited-volume specimens from PBMC cultures or pediatric draws while supporting high-throughput screening of stimulation conditions.

This kit is directly applicable to tuberculosis immune monitoring, transplant rejection surveillance, and Th1/Th2 polarization studies.

Related Products

Target Background

Function
(From Uniprot)

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.

Gene References into Functions

Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages. PMID: 29249666
High expression of IFNG is associated with breast cancer. PMID: 30336781
results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
IFN-lambda may participate in local inflammation in the salivary glands of primary Sjogren's syndrome patients through direct and indirect regulations of the expressions of BAFF and CXCL10 in salivary gland epithelium. PMID: 28421993
The expression of CXCL10 mRNA and IFN-gamma mRNA was significantly higher in non-lesional and perilesional skin of vitiligo and alopecia areata patients compared with the skin of healthy controls; however, the level of expression of CXCL10 and IFN-gamma in lesional skin was not different than that in healthy skin PMID: 27863059
High IFNG expression is associated with Chronic Periodontitis. PMID: 30051674
hypomethylation of the IFNG promoter is significantly related to the risk of essential hypertension PMID: 29643275
These results demonstrated that the IFNGinduced immunosuppressive properties of B7H1 in human BM and WJMSCs were mediated by STAT1 signaling, and not by PI3K/RACalpha serine/threonineprotein kinase signaling PMID: 29901104
serum IP-10 level and the IFN-gamma/IL-4 ratio have great potential to predict significant fibrosis among chronic hepatitis B patients. PMID: 28067328
IFN-gamma increases free ISG15 levels in the cytoplasm and ISGylation in the nucleus and cytoplasm, but in a manner distinct between MCF-7 and MDA-MB-231cells. PMID: 29626479
the expression of IFN-gamma and IL-17 was also suppressed by IRAK1/4 inhibitor both in active Behcet's patients and in normal subjects. PMID: 28780618
IFNgamma induces a rapid activation of aerobic glycolysis followed by a reduction in oxidative phosphorylation in M1 macrophages. PMID: 29463472
Results provide evidence that rs2069707 locus SNPs of IFN-gamma is a risk factor for contracting tuberculous pericarditis. PMID: 30017738
No correlation was observed between interferon gamma mRNA/protein levels and recurrent depressive disorders. PMID: 29367100
Aberrant IFN-gamma promoter methylation may be involved in the process of tumorigenesis of oral cancer. PMID: 28091876
this study shows that elevated levels of interferon-gamma are associated with high levels of Epstein-Barr virus reactivation in patients with the intestinal type of gastric cancer PMID: 29349089
This study contributes to clarification of the previously inconsistent prognostic performance of IFNgamma by providing the first prognostic evaluation with long follow-up, time-dependence assessment and absence of any chemotherapy influence. PMID: 29478965
Association Between the Interferon Gamma 874 T/A Polymorphism and the Severity of Valvular Damage in Patients with Rheumatic Heart Disease. PMID: 29332266
IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
an electrophoretic mobility shift assay showed that signal transducers and activators of transcription 1 (STAT1) attach to the GAS motif on the human STING promoter region. This indicates that IFN-gamma/Janus kinases/STAT1 signaling is essential for the STING upregulation in human keratinocytes. PMID: 29143896
we review the direct and indirect effects of IFN-gamma on hematopoiesis, as well as the underlying signaling mechanisms of how IFN-gamma modulates the self-renewal, cell cycle entry, and proliferation of hematopoietic stem cells PMID: 28852997
IFN-gamma +874T allele may increase the risk of ocular lesions in Toxoplasma infection. The principle of natural selection seems to also play a role. The less common TNF-308A allelic form could be protective against the development of Toxoplasma ocular infection. PMID: 27081842
this study shows the age-related reductions in serum and PBMC IFN-gamma in healthy nonobese subjects PMID: 28762199
Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
the frequencies of IFNgamma and IL-17A(+) cells were increased in the antrum, particularly in patients with H. pylori induced gastric ulcers. PMID: 28683359
rs2069718 in the IFNG gene was significantly associated with pulmonary tuberculosis but not spinal tuberculosis. PMID: 28867622
IFN-gamma was associated with a cerebral volume reduction in systemic lupus erythematosus with central nervous system involvement PMID: 28848179
Data suggest that semen exhibits substantial individual variation over time in pro-inflammatory seminal fluid cytokines IFNG and CXCL8. (IFNG = interferon gamma; CXCL8 = C-X-C motif chemokine ligand 8) PMID: 28541460
Dysregulation of the IFN-gamma-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia PMID: 29474442
STAT1b plays a key role in enhancing the tumor suppressor function of STAT1a, in ESCC, in a manner that can be amplified by IFN-gamma PMID: 28981100
epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
Systemic IFN activation is associated with higher activity only in the ESSDAI biological domain but not in other domains or the total score. Our data raise the possibility that the ESSDAI biological domain score may be a more sensitive endpoint for trials targeting either IFN pathway. PMID: 29474655
These findings suggest that IFN-a can inhibit HCV replication through a STAT2-dependent but STAT1-independent pathway, whereas IFN-g induces ISG expression and inhibits HCV replication exclusively through a STAT1- and STAT2-dependent pathway. PMID: 27929099
rs1861493 and rs2430561 polymorphisms were conformed to be in HWE in genotypes distribution of the control group (P>0.05 for both). However, only TT genotype and T allele of rs2430561 presented significantly higher frequencies in Ankylosing Spondylitis patients than in healthy controls. IFN-g rs2430561 polymorphism may contribute to the risk of IFN-g rs2430561 polymorphism may contributhrough influencing IFN-g expression. PMID: 28843049
Interactions among polymorphisms of IFN-gamma+874 AA, IL-2-330 TT, IL-10-1082 AA, IL10--592 AC and IL-4-589 CC/CT significantly influenced the clinical progression of the subjects with hepatitis B virus and/or hepatitis C virus infection. PMID: 28838891
Ex vivo interferon-gamma production is a useful biomarker for assessing disease activity and predicting poor clinical outcomes of systemic lupus erythematosus patients. PMID: 28841837
this study demonstrates reduced IFN-gamma production in chronic brucellosis patients PMID: 28919584
These data suggest that the de novo expression of PDL1 on tumor cells is upregulated by IFN-g secreted from CD8+ TILs upon recognition of the tumor cells with an MHC class I molecule. PMID: 28791392
H pylori expression of cgt reduces cholesterol levels in infected gastric epithelial cells and thereby blocks IFNG signaling, allowing the bacteria to escape the host inflammatory response. PMID: 29273450
Strategies to block MICA-NKG2D interactions resulted in reductions in IFNgamma production. Depletion of monocytes in vivo resulted in decreased IFNgamma production by murine NK cells upon exposure to Ab-coated tumor cells PMID: 28724544
study concluded that the IFN-gamma (874A/T) polymorphism is associated with the susceptibility to oral lichen planus PMID: 27544215
IFN gamma induced upregulation of BCL6 was dependent on the classical STAT1 signaling pathway, and affected both major BCL6 variants. Interestingly, although IFN alpha induced stronger STAT1 phosphorylation than IFN gamma, it only slightly upregulated BCL6 in multiple myeloma lines. PMID: 29510136
IFN-gamma, CXCL16 and uPAR are promising as effective biomarkers of disease activity, renal damage, and the activity of pathological lesions in systemic lupus erythematosus. PMID: 28628472
serum levels of soluble FAS ligand (sFASL) and interferon gamma (IFN-gamma) were analyzed and correlated with sFGL2 levels in Hepatitis C Virus-Infected Patients and Hepatocellular Carcinoma Patients. PMID: 28609212
IFN-gamma induces activated but insufficient autophagy and thus contributes to a degree to p62-dependent apoptosis of nasal epithelial cells in chronic rhinosinusitis with nasal polyps. PMID: 28258963
results suggested that IFN-gamma induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production. PMID: 29551681
Activated interferon-gamma-producing CD56(bright) NK cells are positioned to play a key role in the fibrotic process and progression to chronic kidney disease. PMID: 28396119
Posttransplant immune monitoring by donor-specific IFN-gamma ELISPOT can assess risk for developing subclinical T-cell mediated rejection and anti-donor HLA antibodies. PMID: 28274484
genetic polymorphism is not associated with increased susceptibility to chronic spontaneous urticaria in Iran PMID: 28159384

Hide All

Involvement in disease

Aplastic anemia (AA)

Subcellular Location

Secreted.

Protein Families

Type II (or gamma) interferon family

Tissue Specificity

Released primarily from activated T lymphocytes.

Database Links

HGNC: 5438

OMIM: 147570

KEGG: hsa:3458

STRING: 9606.ENSP00000229135

UniGene: Hs.856

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Code	CSB-E04577h-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Human Interferon γ (IFN-γ) ELISA Kit

Product Details

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Customer Reviews and Q&A

Target Background