Code | CSB-E04577h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||
| Sample | Serum(n=4) | |||
1:1 | Average % | 92 | |||
Range % | 87-96 | ||||
1:2 | Average % | 95 | |||
Range % | 92-99 | ||||
1:4 | Average % | 91 | |||
Range % | 87-94 | ||||
1:8 | Average % | 89 | |||
Range % | 86-93 | ||||
The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||
Sample Type | Average % Recovery | Range | |||
Serum (n=5) | 91 | 88-96 | |||
EDTA plasma (n=4) | 95 | 89-99 | |||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
500 | 2.046 | 2.058 | 2.052 | 1.902 | ||
250 | 1.537 | 1.444 | 1.491 | 1.341 | ||
125 | 1.027 | 0.987 | 1.007 | 0.857 | ||
62.5 | 0.648 | 0.635 | 0.642 | 0.492 | ||
31.2 | 0.456 | 0.432 | 0.444 | 0.294 | ||
15.6 | 0.342 | 0.345 | 0.344 | 0.194 | ||
7.8 | 0.241 | 0.254 | 0.248 | 0.098 | ||
0 | 0.151 | 0.149 | 0.150 |
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