Code | CSB-E08053h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 103 | ||||
Range % | 98-108 | |||||
1:2 | Average % | 99 | ||||
Range % | 92-105 | |||||
1:4 | Average % | 97 | ||||
Range % | 92-104 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 100 | 95-106 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
150 | 3.049 | 2.934 | 2.991 | 2.864 | |||
75 | 2.404 | 2.467 | 2.436 | 2.308 | |||
37.5 | 1.507 | 1.528 | 1.517 | 1.390 | |||
18.75 | 0.845 | 0.829 | 0.837 | 0.710 | |||
9.375 | 0.475 | 0.451 | 0.463 | 0.335 | |||
4.688 | 0.300 | 0.293 | 0.296 | 0.169 | |||
2.344 | 0.221 | 0.214 | 0.218 | 0.090 | |||
0 | 0.130 | 0.125 | 0.128 |
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