Code | CSB-E04593h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 94 | ||||
Range % | 89-100 | |||||
1:2 | Average % | 99 | ||||
Range % | 92-106 | |||||
1:4 | Average % | 90 | ||||
Range % | 86-96 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 88-101 | ||||
EDTA plasma (n=4) | 89 | 83-96 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
250 | 2.785 | 2.634 | 2.709 | 2.546 | |||
125 | 2.243 | 2.107 | 2.175 | 2.012 | |||
62.5 | 1.398 | 1.448 | 1.423 | 1.260 | |||
31.25 | 0.900 | 0.929 | 0.914 | 0.751 | |||
15.6 | 0.552 | 0.541 | 0.546 | 0.383 | |||
7.8 | 0.410 | 0.403 | 0.406 | 0.243 | |||
3.9 | 0.294 | 0.284 | 0.289 | 0.126 | |||
0 | 0.162 | 0.165 | 0.163 |
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