Code | CSB-RA011580A0HU |
Size | US$210 |
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The production of the IL10 recombinant monoclonal antibody involves the use of DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human IL10, and B cells are isolated and screened to select positive ones. Single clone identification is performed to ensure specificity. The light and heavy chains of the IL10 antibody are amplified through PCR and then incorporated into a plasmid vector. This recombinant vector is introduced into a host cell line to enable antibody expression. The IL10 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It exhibits high reactivity with human IL10 protein and is suitable for ELISA.
IL10 is an anti-inflammatory cytokine that plays an important role in regulating immune responses and preventing excessive inflammation. In cells, IL10 can bind to its receptor on the surface of various immune cells, including T cells, B cells, monocytes, and macrophages. This interaction triggers a signaling cascade that results in the inhibition of the production of pro-inflammatory cytokines such as IL-1, IL-6, and TNF-alpha. IL10 can also promote the differentiation and activation of regulatory T cells, which can further dampen immune responses.
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