Recombinant Human Interleukin-10 protein(IL10) (Active)

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Code CSB-AP001791HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Target Names IL10
Uniprot No. P22301
Research Area Immunology
Alternative Names CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; MGC126450; MGC126451; T-cell growth inhibitory factor; TGIF
Species Homo sapiens (Human)
Source E.coli
Expression Region 19-178aa
Complete Sequence SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Mol. Weight 18.6 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Gene References into Functions
  1. Autocrine IL10 regulates Op18/stathmin signaling via an IL10NFkappaBERK/CDC2 axis. PMID: 30320402
  2. CD163(+)CD204(+) Tumor-associated macrophages possibly play a key role in the invasion and metastasis of oral squamous cell carcinoma by T-cell regulation via IL-10 and PD-L1 production. PMID: 28496107
  3. study showed that the rs1800872 A allele of the IL-10 gene may contribute to the genetic susceptibility of Behcet's disease by regulating the expression of IL-10. PMID: 29294320
  4. study indicates that anti-inflammatory macrophage function and mucosal immune tolerance require both WASP and DOCK8, and that IL-10 signalling modulates a WASP-DOCK8 complex PMID: 29725003
  5. This study demonstrated the alteration of IL-10 levels in aseptic non-vasculitic cerebral sinovenous thrombosis PMID: 30246697
  6. The interleukin-10 gene promoter rs1800872 single nucleotide polymorphism is associated with predisposition to chronic hepatitis C in case of infection with hepatitis C virus 1b genotype in the Russian population. PMID: 29247709
  7. Mycobacterium tuberculosis infection disturbs the HDAC6/HDAC11 levels to induce IL-10 expression in macrophages. PMID: 29523311
  8. The results of this meta-analysis indicated that there is no association between the IL-10 -592 A/C promoter polymorphism and gastrointestinal tract cancer susceptibility. PMID: 29720026
  9. Low IL10 expression is associated with dyslipidaemia, high blood pressure and glucose intolerance. PMID: 29615317
  10. Regulatory polymorphism in the IL-10 gene promoter has a possible and significant association with severity and outcome in patients with acute viral hepatitis, and acute liver failure PMID: 30109600
  11. These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis. PMID: 28216665
  12. Individuals with posttraumatic stress disorder showed a significant increase in the serum levels of IL-10 (and IL-6). PMID: 29179015
  13. IL-10 SNP at -819 was associated with enhanced AML risk, suggesting that rs1800871 provides clue for future studies and early detection of Acute myeloid leukemia . PMID: 30197353
  14. Although IL-10 promoter polymorphisms are not associated with P. falciparum and S. haematobium co-infection, variant -1082G/A and haplotype GCC are associated with malaria, whereas the IL-10 haplotypes GCC and GTA are associated with schistosomiasis. PMID: 29131459
  15. high IL-10 in rheumatoid arthritis is associated with increased depressive symptoms PMID: 30148175
  16. besides the NF-kappaB pathway, the mechanism of action of FGF-21 was observed to involve the elevation of IL-10 in the ERK1/2 pathway. PMID: 29427162
  17. meta-analysis strongly suggests that the IL10 rs1800896, rs1800871, and rs1800872 polymorphisms are not associated with the risk of autoimmune liver disease PMID: 29694797
  18. Single nucleotide polymorphisms (SNPs) rs12979860 and rs8099917 (IL28B) and rs1800896, rs1800871, and rs1800872 (IL10) are related to treatment outcome, but previous studies clustered nonresponse and relapse patients.Frequency of rs12979860 and rs8099917 is different between relapsers and nonresponders, but similar between relapsers and responders PMID: 29888255
  19. The aim of this study was to explore the possible correlations of serum interleukins and soluble ST2 (sST2) protein with clinical features and inflammatory cytokines in rheumatoid arthritis (RA) patients. PMID: 29798971
  20. Anti-inflammatory cytokines, IL-10 and TGF-beta1, are not upregulated in the serum of patients with juvenile idiopathic arthritis. PMID: 29494710
  21. Overall, no significant association between IL-10 -592 A/C polymorphism and Kawasaki disease risk was found under allele contrast. The IL-10 -592 A/C polymorphism was not associated with Kawasaki disease risk in the Chinese population. PMID: 29656715
  22. These results suggest that the IL-10 -1082 G/A polymorphism confers susceptibility to juvenile idiopathic arthritis. PMID: 29748155
  23. Data show that multiple sclerosis TH1/17 cells have reduced expression of interleukin 10 (IL10) compared to healthy controls. PMID: 29150604
  24. High IL10 and Tumor necrosis factor-alpha expression is associated with coronary artery disease. PMID: 28988596
  25. Results discovered that plasma IL-10 levels in acute respiratory distress syndrome patients were correlated with severity of illness during extracorporeal membrane oxygenation institution. Also, high interleukin-10 was found to be associated to two polymorphic nucleotides at the gene promoter area. PMID: 28432351
  26. association of gene polymorphism (-819C > T, -592C > A and -1082G > A) with preterm birth PMID: 29147889
  27. Suggest that the IL-10 -819T/T variant and the ATA haplotype, which are associated with low production of IL 10, represent genetic risk factors for preeclampsia in Tunisian women. PMID: 29523264
  28. The IL-10 rs1800890 variant might contribute to RA susceptibility in the Chinese population. PMID: 29487192
  29. findings support the hypothesis that the -1082A>G polymorphism in the IL10 gene might be associated with diabetic kidney disease in white Brazilians with type 2 diabetes. PMID: 29227971
  30. This study showed that the activated FcgammaRII(low/-) B cells from HCC tumours, but not the resting FcgammaRII(high) B cells, without external stimulation suppress autologous tumour-specific cytotoxic T-cell immunity via IL-10 signals. PMID: 27853178
  31. It was shown that the expression of CD274, Il-10, and FOXP3 was closely correlated with the clinical characteristics of laryngeal squamous cell carcinoma, including lymph node metastasis and prognosis. PMID: 29257349
  32. IL-10 mRNA levels in peripheral B cells were significantly higher in lung cancer patients.A negative correlation was identified between miR-98 and IL-10 in peripheral B cells. PMID: 27605397
  33. These findings added to the accumulating evidence that promoter haplotypes of IL-10 may be important modulators of the development of amnestic mild cognitive impairment PMID: 29201645
  34. Findings suggest that the combinatorial complexity of TNFA and IL10 promoter polymorphisms impacts systemic lupus erythematosus susceptibility. PMID: 29298134
  35. The combination of IL10 and DAO SNPs in a multivariate model did not alter the OR values. PMID: 28750137
  36. The results indicate that IL10-1082 A/G polymorphism is associated with genetic susceptibility/predisposition to Rheumatoid arthritis in North Indian population. PMID: 29621504
  37. IL-33 increased IL-10 expression in MFCs via activating ERK 1/2 and STAT3, which subsequently promoted IL-10 transcription and thus contributed to the beneficial effects of IL-33 on MFCs. PMID: 29099095
  38. theses results suggest that IL-10 genotypes of recipient are the most associated with the risk of complications after haematopoietic stem cell transplantation PMID: 28987962
  39. Our results suggest that genetic variation in IL-10 gene is unlikely to confer susceptibility to Henoch-Schonlein purpura in Chinese children. PMID: 28963667
  40. neither allele nor genotype frequencies of IL-10 polymorphisms were associated with inflammatory bowel disease in Iranian patients PMID: 28872970
  41. According to our data, TNF A -238G>A and IL-10 -1082A>G, -819C>T and -592C>A may be associated with the development of prostate cancer and BPH. We could also notice higher frequency of TNF A and IL-10 risk haplotypes in smoker and alcohol user. Interestingly, IL-10 risk haplotype was positively associated with aggressiveness of tumor. PMID: 28993831
  42. Local IL-10 and IL-13 upregulation in IgG4-abdominal aortic aneurysms was related to Th2 and Treg-predominant cytokine balance. PMID: 28434701
  43. Suggest that the interaction between macrophages and endometrial stromal cells downregulates cytotoxicity of NK cells possibly by stimulating the secretion of IL-10 and TGF-beta, and may further trigger the immune escape of ectopic fragments and promote the occurrence and the development of endometriosis. PMID: 28971893
  44. isatuximab decreases multiple myeloma cell- and bone marrow stromal cell-induced iTreg by inhibiting both cell-cell contact and TGFbeta/IL10. Finally, CD38 levels correlate with differential inhibition by isatuximab of Tregs from multiple myeloma versus normal donors.Targeting CD38 by isatuximab can preferentially block immunosuppressive Tregs and thereby restore immune effector function against multiple myeloma PMID: 28249894
  45. Activation of PAR2 inhibits the expression of IL-10 in B cells, which can be reversed by treating B cells with Bcl2L12 shRNA-carrying liposomes. PMID: 28426164
  46. Serum IL-17 and IL-23 values positively correlated with serum total IgE levels. Serum levels of IL-10 were lower in children with atopy and dyslipidemia than patients with dyslipidemia. PMID: 28692864
  47. This meta-analysis concludes that IL-10 -1082 gene polymorphism is not significantly associated with overall, Asian and African population. However, this polymorphism is associated with Caucasian population. PMID: 28951522
  48. The data suggest that IL-10, but not IL-37, may have potential as a biomarker predictive for disease activity in Systemic lupus erythematosus. PMID: 27708376
  49. In the study, 250 ONFH patients and 228 matched healthy controls from Shandong Province were recruited to evaluate the influence of interleukin-10 (IL-10) rs1800872, IL-12 rs3212227 and tumor necrosis factor-(TNF-alpha) rs1800629 polymorphism in osteonecrosis of the femoral head (ONFH). IL-12 rs3212227 AC genotype confer genetically susceptibility to ONFH in the Chinese Han population. PMID: 28980933
  50. Gene polymorphisms of TNF-238G/A, TNF-308G/A, IL10-1082G/A, TNFAIP3, and MC4R and comorbidity occurrence in a Romanian population with psoriasis PMID: 29696068
  51. IL10R signaling regulates TH17 polarization and T-cell proliferation in humans but is not required for the generation and in vitro suppression of Tregs. Therapies targeting the TH17 axis might be beneficial for IL10- and IL10R-deficient patients as a bridge to allogeneic hematopoietic stem cell transplantation. PMID: 29023267
  52. Targeting the 'Bregs/IL10/IL10R' system may provide a novel therapeutic approach for the treatment of SLE. PMID: 28901380
  53. There is a significant association between the IL-10-592C/A promoter single nucleotide polymorphism and a decreased risk of colorectal cancer in an ethnic Kashmiri population. PMID: 28538040
  54. genotype distribution of IL-10-1082GG was higher in mild patients than in severe patients PMID: 29094241
  55. There is an independent association of the -1082A>G polymorphism in the IL10 gene with non-proliferative diabetic retinopathy in type 2 diabetes. This finding provides additional evidence supporting that genetic variants of IL10 are involved in the pathogenesis of diabetic retinopathy. PMID: 29382585
  56. the IL-10-592C allele was observed with higher frequency in patients with critical Enterovirus 71 (EV71) infection compared with severe EV71 infection, mild EV71 infection and healthy children PMID: 28843383
  57. INSR rs1051690 SNP is associated with increased risk of gastric cancer, while polymorphisms in IL12B, CCND1 and IL10 genes are not linked with the presence of gastric cancer PMID: 28596683
  58. use of MCP-1/IL-10 ratio in combination with ultrasound findings appears to provide a promising modality for predicting preeclampsia PMID: 28038968
  59. tis study shows that CXCR5(+)CD8(+) T cells present elevated capacity in mediating cytotoxicity toward autologous tumor cells through interleukin 10 in diffuse large B-cell lymphoma PMID: 28662433
  60. results suggest that two immune biomarkers, CD74 and IL10, could be relevant tools for the identification of Intensive care unit-acquired infections risk in Intensive care unit patients. PMID: 28477143
  61. The authors suggest that the -592C > A polymorphism in IL-10 gene might be a susceptibility locus for colorectal cancer in East Asians. PMID: 27489033
  62. IL-10 level and polymorphisms as well as disturbed T-cell subsets percentages are demonstrable effectors of immune dysfunction in immune thrombocytopenia PMID: 29696721
  63. the present study indicated that high levels of miR-19a and low levels of IL-10 were observed in peripheral DCs in patients with nasal polyp and allergic rhinitis. PMID: 28388587
  64. this meta-analysis suggests that IL-10 -1082 A/G (rs1800896) polymorphism may be associated with increased gastric cancer risk. Moreover, further studies estimating the effect of gene-gene and gene-environment interactions are necessary to better understanding of the association between the IL-10 -1082 A/G (rs1800896) polymorphism and risk of gastric cancer. PMID: 29561974
  65. IL-10 gene promoter polymorphisms may act as a major genetic regulator in the etiology of spontaneous abortions with maternal genome imprinting effects PMID: 28456881
  66. Findings indicate that IL-10 act as a negative regulator of IL-33/ST2 signaling pathways in vivo. PMID: 28415811
  67. Study provides evidence that Gzmb is a key characteristic of all CD25++ regulatory T cells. PMID: 27756896
  68. IL-10 produced by unstimulated CD4+CD25+ T cells was significantly higher in the healthy group versus those from patients with gestational diabetes. PMID: 29298097
  69. Breast milk immunomodulators TGFbeta1 and TGFbeta2 were significantly associated with neonatal gut microbial composition (R = 0.024, P = 0.041; R = 0.026, P = 0.012, respectively) and increased richness, evenness, and diversity, but IL-10 was not. The effects of TGFbeta1 and TGFbeta2, however, were not independent of one another, and the effect of TGFbeta2 was stronger than that of TGFbeta1. PMID: 28827481
  70. IL-10 AA genotype carriers had lower IL-6 and IL-6/10 levels in serum; however, GCF IL-6/10 levels were similar in both genotypes. Within the limitations of our study, a possible association between IL-10(-597) gene polymorphism and chronic periodontitis might be considered. PMID: 29489938
  71. Detectable serum IL10 levels were significantly associated with Epstein-Barr virus-positive classical Hodgkin lymphoma cases. PMID: 28341757
  72. HDAC11 was initially identified as a negative regulator of the well-known anti-inflammatory cytokine IL-10. Hence, antagonizing HDAC11 activity may have anti-tumor potential, whereas activating HDAC11 may be useful to treat chronic inflammation or autoimmunity. PMID: 29222071
  73. IL-10 gene -592A>C polymorphism may represent a promising candidate locus for the occurrence of PCa, and further signified a contributing role of this polymorphism in prostate carcinogenesis. PMID: 28526808
  74. IL17F (rs2397084) and IL10 (rs1800871) genes are associated with functional dyspepsia. PMID: 28965252
  75. results demonstrated a dual association of serum IL-10 levels in the initiation and progression of CRC. While lower IL-10 levels were associated with higher risk of the disease, its higher levels were associated with a poorer prognosis PMID: 28643743
  76. The authors results indicate that genetic variants of the immunomodulatory IL10 and IL20 genes may offer a protective effect in Europeans from Russia. PMID: 28150860
  77. Circulating anti-inflammatory IL-10 is increased following burst spinal cord stimulation for failed back surgery patients. PMID: 28194840
  78. Report presence of low frequency Il10 genetic variants in Behcet's disease patients. PMID: 24708170
  79. FVIII inhibitor formation in a group of Egyptian hemophilia A patients was not correlated to IL10-1082A/G polymorphism, disease severity, or any of the treatment variables. PMID: 28574805
  80. Higher IL-10 blood level in cardiovascular disease patients and il-10 promoter single nucleotide polymorphism association with cardiovascular disease PMID: 28474494
  81. The findings suggest that IL-10 c.-592C > A polymorphism is independently associated with human papillomavirus infections (HPV) susceptibility exerting influence on IL-10 cervical levels in HPV infected women, thus contributing to cervical carcinogenesis. PMID: 28552689
  82. these results suggest that that C-reactive protein may be a potent inducer of the anti-inflammatory cytokine IL-10 PMID: 28619036
  83. TonEBP suppresses M2 phenotype via downregulation of the IL-10 in M1 macrophages. PMID: 27160066
  84. IL28B (rs8099917 and rs12979860) and IL10 (rs1800896) polymorphisms alone, or in combination, are good predictors of therapeutic response in hepatitis C virus-3a patients. PMID: 29340806
  85. IL-10 1082GG genotype was associated with lower sepsis odds in very low-birth-weight (VLBW) infants. We uncovered an association between IL-10 1082 gene variation and sepsis in VLBW infants but did not identify associations between neonatal sepsis and TNF-alpha 308 or IL-6 gene variation. [review] PMID: 27960200
  86. In the type 2 diabetes mellitus (T2DM) group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype PMID: 28225860
  87. this meta-analysis shows that interleukin-10 rs1800896 polymorphism is associated with head and neck cancer risk PMID: 28410223
  88. There was no significant difference in FOXP3, RORgammat, IL-10 protein expression and supernatant PBMCs IL-10 in chronic heart failure patients as compared to control. PMID: 29198133
  89. When comparing IBS subtypes, TNFalpha and IL-17 were significantly (P<0.05) higher, and IL-10 was significantly (P<0.05) lower in diarrhea predominant irritable bowel syndrome (IBS-D) compared to HCs, whereas the inflammatory cytokine profile of other subtypes more closely resembled that of HCs. PMID: 28506572
  90. results suggest that IL-10 is a susceptibility gene in patients withIgA nephropathy . PMID: 28359052
  91. These results show that vaccine-induced immunoregulatory IL- 10+ DC impair priming of antitumor immunity, suggesting that therapeutic vaccination protocols may benefit from combined targeting of inhibitory molecules expressed by this DC subset. PMID: 27926522
  92. Results indicate that IL-10 and TGF-beta are involved in HLA-E-mediated NB migration and invasion. PMID: 27322426
  93. Foxp3(+) cells in the brains of Multiple sclerosis patients predominantly produce interleukin-10 (IL-10) and show high expression of the IL-33 receptor ST2. PMID: 28169287
  94. evidence of a deregulation of the IL10 expression in peripheral blood mononuclear cells of patients with rheumatoid arthritis and the haplotypes evaluated may be contributing slightly to this condition. PMID: 28254559
  95. Our meta-analysis indicated that the IL10-1082 G/A polymorphism is associated with recurrent aphthous stomatitis susceptibility, especially in Asians PMID: 29384969
  96. Suggest that IL-10 rs1800871 and PRKDC rs7003908 may be useful biomarkers for predicting glioma patient survival. PMID: 27811370
  97. Its single nucleotide polymorphisms plays a role in dementia pathogenesis by increasing risk of developing motoric cognitive risk syndrome in older adults. PMID: 28705468
  98. In vitro deletion of A20 compromised the expression of IL10 in B regulatory cells. PMID: 27825134
  99. IL-10 from M2 macrophage promoted proliferation of glioma through interaction with JAK2. PMID: 27765933
  100. Primary open angle glaucoma in type 2 diabetes: Implications of the IL-10/STAT3-mediated anti-inflammatory response PMID: 27693215

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Subcellular Location Secreted
Protein Families IL-10 family
Tissue Specificity Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Database Links

HGNC: 5962

OMIM: 124092

KEGG: hsa:3586

STRING: 9606.ENSP00000412237

UniGene: Hs.193717

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