Purity
>95% as determined by SDS-PAGE and HPLC.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Alternative Names
CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL 10; IL-10; IL10; IL10_HUMAN; IL10A; Interleukin 10; Interleukin-10; MGC126450; MGC126451; T-cell growth inhibitory factor; TGIF
Species
Homo sapiens (Human)
Expression Region
19-178aa
Complete Sequence
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Protein Length
Full Length of Mature Protein
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Human Interleukin-10/IL10 was generated by the expression of a DNA fragment encoding 19-178aa of human IL10 in E.coli. The full-length of mature protein reached up to 95% in purity determined by SDS-PAGE and HPLC analyses. It was also verified to be fully biologically active through its effect on a cell proliferation assay using murine MC/9-2 cells (ED50<1 ng/ml, specific activity >1.0x106 IU/mg). The endotoxin of this IL10 is less than 1.0 EU/μg as determined by the LAL method. Proteins in-stock are offered now. The IL10 proteins are devoid of any tag, but CUSABIO offers custom service for you to meet the need of specific tag(s). This recombinant IL10 protein may be used to produce anti-IL10 antibodies or in the immunology studies.
IL10 is an immunosuppressive cytokine that plays a key role in regulating intestinal immune homeostasis. IL10 functions by binding to the IL10 receptor. The activation of STAT3 by IL10 is essential for the immunosuppressive effects of IL10. Animal studies have shown that IL10 controls the responses of effector Th1 and Th17 thus maintaining intestinal tolerance to microbiota. It has recently demonstrated that the failure of innate immune cells to respond to IL-10 is critically involved in the development of such T cell-driven intestinal inflammation. S. Veenbergen etc. demonstrated that the IL-10 signaling in dendritic cells controls IL-1β-mediated IFNγ secretion by human CD4+ T cells.