Human Interleukin 10,IL-10 ELISA KIT

Human Interleukin 10,IL-10 ELISA KIT

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IL10 Antibodies

IL10 Proteins

IL10 Antibodies

IL10 Proteins

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Human Interleukin 10,IL-10 ELISA KIT

Specification
Target

Code

CSB-E04593h

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P22301

Lead Time

3-5 working days

Abbreviation

IL10

Target Name

interleukin 10

Alias

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Species

Homo sapiens (Human)

Sample Types

serum, urine, cell culture supernates, ascitic fluid, cerebrospinal fluid (CSF), saliva

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

HGNC

5962

RGD

2886

MGI

96537

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-100
1:2	Average %	100
	Range %	91-105
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.573	2.593	2.583	2.435
400	2.045	2.000	2.023	1.875
200	1.439	1.456	1.448	1.300
100	0.880	0.899	0.890	0.742
50	0.631	0.652	0.642	0.494
25	0.392	0.421	0.407	0.259
12.5	0.245	0.256	0.251	0.103
0	0.142	0.153	0.148

References

Immunotoxicity of pyrethroid metabolites in an in vitro model /,Environmental Toxicology and Chemistry,2010 Nov 29(11):2505-10.
Interaction of coagulation factors and tumor-associated macrophages mediates migration and invasion of gastric cancer Ma YY et al,International Journal of Rheumatic Diseases,2011 Feb;102(2):336-42
Efficient Attenuation of NK Cell-mediated Liver Injury through Genetically Manipulating Multiple Immunogenes by Using a Liver-Directed Vector Geng J et al,J Immunol,2013 May 1;190(9):4821-9.
The Relationship among Interlukin-10 ,Interlukin-6 with anti-cyclic citrullinated peptide antibody, C-reactive protein ,Rheumatoid factor in patients with Rheumatoid Arthritis Ahmed Abbas Hasan.et al,AL-Qadisiya Medical Journal ,Mar 22
Kinin B1 receptor as a novel, prognostic progression biomarker for carotid atherosclerotic plaques Liu .et al,Mol Med Rep. ,Oct 3
An immunosuppressive function of interleukin-35 in chronic hepatitis C virus infection Liu S.et al,Int Immunopharmacol.,Jun 20
PKN2 in colon cancer cells inhibits M2 phenotype polarization of tumor-associated macrophages via regulating DUSP6-Erk1/2 pathway Cheng Y.et al,Mol Cancer,Jan 24
Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans Wasfi R.et al,J Cell Mol Med,Jan 8
Reduced peripheral blood regulatory B cell levels are not associated with the Expanded Disability Status Scale score in multiple sclerosis Shujun Guo.et al, Journal of International Medical Research,July 19
6-months Clinical Outcomes of Non-Surgical Periodontal Treatment with Antibiotics on Apoptosis Markers in Aggressive Periodontitis Aral K, et al,Oral Diseases,January 6
IL-10 is associated with increased mu-opioid receptor levels in major depressive disorder Al-Fadhel SZ, et al,European Psychiatry,January 21

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Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Database Links	HGNC: 5962 OMIM: 124092 KEGG: hsa:3586 STRING: 9606.ENSP00000412237 UniGene: Hs.193717
Pathway	Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

IL10 Antibody

IL10 Protein

IL10 Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-RA011580A0HU	IL10 Antibody	Human	ELISA

IL10 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA011580KA01HU	IL10 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA004870	IL10 Antibody	Human	WB,ELISA

IL10 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP011580HU CSB-EP011580HU CSB-BP011580HU CSB-MP011580HU	Recombinant Human Interleukin-10(IL10)	Yeast E.coli Baculovirus Mammalian cell
CSB-AP001791HU	Recombinant Human Interleukin-10 protein(IL10) (Active)	E.coli
CSB-RP065944h	Recombinant Human Interleukin-10(IL10)	E.coli

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.