Human Interleukin 10,IL-10 ELISA KIT

Code CSB-E04593h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22301
Lead Time 3-5 working days
Abbreviation IL10
Target Name interleukin 10
Alias CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Species Homo sapiens (Human)
Sample Types serum, urine, cell culture supernates, ascitic fluid, cerebrospinal fluid (CSF), saliva
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
HGNC 5962
RGD 2886
MGI 96537
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 85-100  
1:2 Average % 100  
Range % 91-105  
1:4 Average % 97  
Range % 92-102  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.573 2.593 2.583 2.435  
400 2.045 2.000 2.023 1.875  
200 1.439 1.456 1.448 1.300  
100 0.880 0.899 0.890 0.742  
50 0.631 0.652 0.642 0.494  
25 0.392 0.421 0.407 0.259  
12.5 0.245 0.256 0.251 0.103  
0 0.142 0.153 0.148    
References
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Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location Secreted
Protein Families IL-10 family
Tissue Specificity Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Database Links

HGNC: 5962

OMIM: 124092

KEGG: hsa:3586

STRING: 9606.ENSP00000412237

UniGene: Hs.193717

Pathway Jak-STAT signaling pathway
T cell receptor signaling pathway
FoxO signaling pathway

Q&A and Customer Reviews

Q&A
Q:

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
Kindly provide the ELISA kit for analysis of Interleukin IL- 10.
How many sample can be analysed by each kit ?

A:
Thanks for your inquiry.
#CSB-E04593h,Human Interleukin 10,IL-10 ELISA KIT
It is applied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
The datasheet of the 4 kits are all attached for your information.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.
Q:

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
Kindly provide the ELISA kit for analysis of Interleukin IL- 10. How many sample can be analysed by each kit ?

A:
Thanks for your inquiry.
#CSB-E04593h,Human Interleukin 10,IL-10 ELISA KIT
We have this kit available. It is applied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.
Customer Reviews

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