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24T ELISA Kit Trial Size (Only USD$150/ kit)
This human IL-10 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-10 in the serum, plasma, or tissue homogenates. Antibody specific for IL-10 has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated IL-10 antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of IL-10 bound in the initial step.
IL-10 is a potent anti-inflammatory cytokine that protects the host from over-exuberant responses to pathogens and microbiota, thus keeping normal tissue homeostasis. IL-10 binding to the heterotetrameric IL-10R complex results in JAK1- and TYK2-mediated phosphorylation of STAT3, leading to the implementation of gene transcription programs and consequent anti-inflammatory and immunosuppressive cellular responses. Excessive IL-10 production can inhibit the pro-inflammatory response to various pathogens and contribute to pathogens escaping from immune control, leading to fulminant and rapidly fatal or chronic non-healing infections. Deficiency of IL-10 is often initially beneficial to the host, prolonged IL-10 absence is linked to enhanced immunopathology in response to infection as well as increased risk for the development of many autoimmune diseases and inflammatory bowel disease.
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I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
Kindly provide the ELISA kit for analysis of Interleukin IL- 10.
How many sample can be analysed by each kit ?