Human Interleukin 10,IL-10 ELISA KIT

Human Interleukin 10,IL-10 ELISA KIT

Q&A and Customer Reviews

Related Products

Most popular with customers

CloseMessage

Specification

Target

IL10 Antibodies

IL10 Proteins

Your Good Partner in Biology Research

Tel : 301-363-4651

Home
Kit
ELISA Kit
Human Interleukin 10,IL-10 ELISA KIT

Specification
Target

Code

CSB-E04593h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P22301

Lead Time

3-5 working days

Abbreviation

IL10

Target Name

interleukin 10

Alias

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

Species

Homo sapiens (Human)

Sample Types

serum, urine, cell culture supernates, ascitic fluid, cerebrospinal fluid (CSF), saliva

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

HGNC

5962

RGD

2886

MGI

96537

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-100
1:2	Average %	100
	Range %	91-105
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.573	2.593	2.583	2.435
400	2.045	2.000	2.023	1.875
200	1.439	1.456	1.448	1.300
100	0.880	0.899	0.890	0.742
50	0.631	0.652	0.642	0.494
25	0.392	0.421	0.407	0.259
12.5	0.245	0.256	0.251	0.103
0	0.142	0.153	0.148

References

Macrophages induce natural killer cell dysfunction via KIR2DL1 during Echinococcus multilocularis infection Bayindala, et al,Tropical Journal of Pharmaceutical Research,2019
Shikonin Controls the Differentiation of CD4+CD25+ Regulatory T Cells by Inhibiting AKT/mTOR Pathway Zhang X,Inflammation,2019
Interleukin-35 modulates the balance between viral specific CD4+CD25+CD127dim/- regulatory T cells and T helper 17 cells in chronic hepatitis B virus infection Yang L, et al,Virology Journal,2019
In Major Depression, Increased Serum Dynorphin and Kappa Opioid Receptor Levels are Positively Associated with Mu Opioid Receptor Levels and Immune Activation and Are Attenuated by Nicotine Dependence Hussein Al-Hakeim, et al,Preprints,2019
Development of a Novel Neuro-Immune and Opioid-Associated Fingerprint with a Cross-Validated Ability to Identify and Authenticate Unknown Patients with Major Depression: Far Beyond Differentiation, Discrimination and Classification Hussein Al-Hakeim, et al,Preprints,2019
IL-10 is associated with increased mu-opioid receptor levels in major depressive disorder Al-Fadhel SZ, et al,European Psychiatry,2019
6-months Clinical Outcomes of Non-Surgical Periodontal Treatment with Antibiotics on Apoptosis Markers in Aggressive Periodontitis Aral K, et al,Oral Diseases,2019
Reduced peripheral blood regulatory B cell levels are not associated with the Expanded Disability Status Scale score in multiple sclerosis Shujun Guo.et al, Journal of International Medical Research,2018
Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans Wasfi R.et al,J Cell Mol Med,2018
PKN2 in colon cancer cells inhibits M2 phenotype polarization of tumor-associated macrophages via regulating DUSP6-Erk1/2 pathway Cheng Y.et al,Mol Cancer,2018
An immunosuppressive function of interleukin-35 in chronic hepatitis C virus infection Liu S.et al,Int Immunopharmacol.,2017
Kinin B1 receptor as a novel, prognostic progression biomarker for carotid atherosclerotic plaques Liu .et al,Mol Med Rep. ,2017
The Relationship among Interlukin-10 ,Interlukin-6 with anti-cyclic citrullinated peptide antibody, C-reactive protein ,Rheumatoid factor in patients with Rheumatoid Arthritis Ahmed Abbas Hasan.et al,AL-Qadisiya Medical Journal ,2017
Efficient Attenuation of NK Cell-mediated Liver Injury through Genetically Manipulating Multiple Immunogenes by Using a Liver-Directed Vector Geng J et al,J Immunol,2013
Interaction of coagulation factors and tumor-associated macrophages mediates migration and invasion of gastric cancer Ma YY et al,International Journal of Rheumatic Diseases,2011
Immunotoxicity of pyrethroid metabolites in an in vitro model /,Environmental Toxicology and Chemistry,2010

Still Have Questions?

Leave a Message or Start an on-line Chat

Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location	Secreted
Protein Families	IL-10 family
Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Database Links	HGNC: 5962 OMIM: 124092 KEGG: hsa:3586 STRING: 9606.ENSP00000412237 UniGene: Hs.193717
Pathway	Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

Q&A

I am planning to carry out a study to assess level of Interleukin from Gingival Crevicular Fluid in Chronic Periodontitis patients before and after treatment.
Kindly provide the ELISA kit for analysis of Interleukin IL- 10.
How many sample can be analysed by each kit ?

Thanks for your inquiry.
#CSB-E04593h,Human Interleukin 10,IL-10 ELISA KIT
It is applied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
The datasheet of the 4 kits are all attached for your information.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.

Thanks for your inquiry.
#CSB-E04593h,Human Interleukin 10,IL-10 ELISA KIT
We have this kit available. It is applied with sandwich enzyme immunoassay technique.For a 96T sandwich ELISA kit (doubleantibody), we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for
the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
If you wan to test Gingival Crevicular Fluid ,we suggest you do pre-testing first.

Hide All

Customer Reviews

Submit CUSABIO Product Reciew in 2 Minutes, Win An Apple Watch!

IL10 Antibody

IL10 Protein

IL10 Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-RA011580A0HU	IL10 Antibody	Human	ELISA

IL10 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA011580KA01HU	IL10 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA004870	IL10 Antibody	Human	WB,ELISA

IL10 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP011580HU CSB-EP011580HU CSB-BP011580HU CSB-MP011580HU	Recombinant Human Interleukin-10(IL10)	Yeast E.coli Baculovirus Mammalian cell
CSB-AP001791HU	Recombinant Human Interleukin-10 protein(IL10) (Active)	E.coli
CSB-RP065944h	Recombinant Human Interleukin-10(IL10)	E.coli

Mouse 1,25-dihydroxyvitamin D3 (DVD/DHVD3) ELISA kit

Detect Range: 1000 fmol/L-5000 fmol/L

Sensitivity: 250 fmol/L
Human α2 macroglobulin,α2 MG ELISA Kit

Detect Range: 1.25 μg/mL-20 μg/mL

Sensitivity: 1.25 μg/mL
Human Arachidonic Acid,AA ELISA Kit

Detect Range: 78.125 ng/mL-5000 ng/mL

Sensitivity: 69.748 ng/mL
Mouse Angiotensin converting enzyme,ACE ELISA Kit

Detect Range: 1.56 ng/mL-100 ng/mL

Sensitivity: 0.39 ng/mL
Mouse adiponectin,ADP ELISA Kit

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL
Human Alpha-fetoprotein,AFP ELISA Kit

Detect Range: 1.563 ng/mL-100 ng/mL

Sensitivity: 1.332 ng/mL
Rat Agrin(AGRN) ELISA kit

Detect Range: 78 pg/mL-5000 pg/mL

Sensitivity: 19.5 pg/mL
Human Agouti Related Protein,AGRP ELISA Kit

Detect Range: 31.25 pg/mL-2000 pg/mL

Sensitivity: 0.002 pg/mL

Product Center

Kit

Protein

Antibody

Molecular Biology Product

Small Molecule

ELISA kit FAQs Four Types of ELISA 11 Tips for Choosing Your Right ELISA Kit How to Do The Pre-experiment before Your ELISA Experiment? ELISA Data Analysis Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Promotions

Products
Service
Technical Resources
Research Tools
About Us
Contact Us
- CUSABIO TECHNOLOGY LLC
- Tel: 301-363-4651
- Email: support@cusabio.com
Join Us with

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How did you know this promotion?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.