Human Transforming Growth factor β3,TGF-β3 ELISA kit

Instructions
Code CSB-E13738h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Human Transforming Growth factor β3 (TGFB3) ELISA kit is specifically designed to standardize the measurement of TGFB3 in biological samples, including serum, plasma, and cell culture supernates, for research and biomedical laboratories. This kit has been validated with various criteria, including sensitivity, specificity, precision (less than 10%), linearity, recovery, and inter-batch difference. The product instructions are access to more details.

The assay principle applied in this kit is the quantitative Sandwich enzyme immunoassay. The microtiter plate has been pre-coated with an anti-human TGFB3 antibody. Standards or samples are added to the appropriate microtiter plate wells with the Biotin-conjugated TGFB3 antibody. Next, HRP-Avidin is added to each microplate well. After TMB substrate solution is added, wells containing TGFB3, Biotin-conjugated TGFB3 antibody, and HRP-conjugated Avidin will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of the stop solution and the color intensity is measured via a microplate reader at 450nm. The levels of TGFB3 in the samples is determined by comparing the O.D. (optical density) of the samples to the standard curve.

TGFB3 is a pleiotropic growth factor exclusively expressed in mammals and mainly secreted and stored as a latent complex in the extracellular matrix (ECM). TGFB3 plays a role in the maturation of myoepithelium, inhibition of milk secretion, and the epithelial-mesenchymal transition (EMT) process. It is essential for the development of the palate and lungs and functions mainly by regulating epithelia-mesenchymal interactions during embryonic, fetal, and neonatal development. Additionally, TGFB3 may also participate in the process of wound closure, orchestrating an orderly migration of dermal and epidermal cells in damaged skin.

Target Name transforming growth factor, beta 3
Alternative Names ARVD ELISA Kit; ARVD1 ELISA Kit; FLJ16571 ELISA Kit; LDS5 ELISA Kit; MGC105479 ELISA Kit; MGC118722 ELISA Kit; prepro-transforming growth factor beta-3 ELISA Kit; RNHF ELISA Kit; TGF beta 3 ELISA Kit; TGF beta3 ELISA Kit; TGF-beta-3 ELISA Kit; TGFB 3 ELISA Kit; Tgfb3 ELISA Kit; TGFB3_HUMAN ELISA Kit; transforming growth factor beta 3 ELISA Kit; Transforming growth factor beta-3 ELISA Kit
Abbreviation TGFB3
Uniprot No. P10600
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-95
1:2Average %97
Range %94-103
1:4Average %98
Range %90-104
1:8Average %87
Range %82-91
Recovery
The recovery of human TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9896-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.699 2.752 2.726 2.571
5002.435 2.399 2.417 2.262
2501.808 1.875 1.842 1.687
1251.065 1.055 1.060 0.905
62.50.678 0.735 0.707 0.552
31.20.401 0.422 0.412 0.257
15.60.267 0.275 0.271 0.116
00.149 0.160 0.155  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TGFB3 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled TGFB3 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • 1 N HCI (1 x 10 ml)--- Used for the acid activation of TGFB3 in the samples.
  • 2 N NaOH/0.5 M HEPES 1 x 10 ml---Neutralize the acidified samples.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Involved in embryogenesis and cell differentiation.
Gene References into Functions
  1. Elevations of TGF-beta3, SMAD2 and SMAD4 in hypertrophic scars and increase of IGF-1R in immature stages may give some clues for acne hypertrophic scar formation. PMID: 30167815
  2. This review highlights advances in the understanding of the cellular sources, activation processes, contextual determinants, and immunological roles of TGF-beta3 with comparisons to other TGF-beta isoforms. PMID: 30071700
  3. This study showed that YOD1 overexpression enhances cell migration by promoting TGF-beta3 signaling which may play an important role in lip and palate formation. PMID: 30145984
  4. We suggest that increased TGFbeta3 levels are responsible for development of aggressive prostate cancer in African American patients as a consequence of development of resistance to inhibitory effects of TGFbeta on cell proliferation and induction of invasive metastatic behavior PMID: 29474521
  5. data implies that miR-140 is a potent chondrogenic differentiation inducer for iPSCs and also, we have showed increasing chondrogenic differentiation by using overexpression of miR-140 and TGFbeta3. PMID: 29456027
  6. The increase in TGF-beta3 found in inflammatory wound healing (WF) highlights its negative effect on wound healing, while the increased levels of sEng in granulating WF affects the leukocyte adhesion/transmigration through the endothelium, reducing the inflammatory response and favoring the wound healing. PMID: 29065449
  7. In nonsyndromic CL+/-P Malay patients,the prevalence of the mutations in the TGFbeta3 gene was 17.7%. For the TGFbeta3 gene, there was no mutation in the coding region in either of the groups. PMID: 26151095
  8. Human dental apical papilla-derived Mesenchymal stem cells (hSCAPs) can produce and secrete TGFbeta3 in response to micro-environmental cues. PMID: 25690385
  9. Higher TGF-beta3 serum concentrations are a risk factor for uterine fibroids. PMID: 27743697
  10. High expression of TGF-beta3 in preeclampsia decidua stimulates miR-494 in decidual mesenchymal stem cells (MSC) and attenuates the regulation of MSC switching the macrophage toward M2 type, contributing to an immune imbalance at maternal-fetal interface. PMID: 27149081
  11. The frequency GA genotype of transforming growth factor beta 3 (TGFbeta3) gene was associated with increased risk of non-syndromic cleft palate only (NS CPO). PMID: 28364787
  12. combining TGF-beta3 with BMP-2 was able to promote the process of bone formation more markedly in vitro, providing a promising clinical strategy in the field of skeletal regeneration and in fracture healing. PMID: 27878265
  13. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes PMID: 27058373
  14. Our study showed that TGFA/TGFB3/MSX1 gene polymorphisms were associated with congenital NSHI. CCGTAC and TTACGT haplotypes might be protective factors, while TTGCGC haplotype might be a risk factor for congenital NSHI. TGFA/TGFB3/MSX1 gene rs3771494, rs1058213, rs3917201, rs2268626, rs3821949, and rs62636562 haplotype analysis showed that haplotype CCGTAC and TTACGT might be protective factors (both P<0.001) PMID: 27356075
  15. increased stromal POSTN induced by TGF-beta3 directly accelerated the growth, migration and invasion of cancer cells PMID: 26857387
  16. Taken together, these results demonstrate that insulin and TGF-beta3 present antagonistic effects during the chondrogenesis of human bone marrow-derived stem/progenitor cells. PMID: 26866713
  17. CCN4 has a positive influence on chondrogenic differentiation by modulating the effects of TGF-beta3. PMID: 26555637
  18. TGFB3 polymorphism is associated with male infertility. PMID: 26612435
  19. Results identified a novel human TGFB3 mutation, which contributes to the clinical delineation of the emerging connective tissue disorder tentatively called Rienhoff syndrome, a disorder overlapping with Marfan and Loeys-Dietz syndrome. PMID: 26184463
  20. Fluocinolone Acetonide enhances TGF-beta3-associated chondrogenesis of bone marrow derived mesenchymal stem cells PMID: 25753754
  21. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells have a role in controlling humoral immune responses PMID: 25695838
  22. TGF-beta3 mediates the attenuating effect of MSCs on both the proliferation and extracellular matrix production of human keloid fibroblasts and decreases skin fibrosis PMID: 25858630
  23. TGF-beta3 existed in synovium and LBs of SC, and was responsible for the pathogenesis of SC. PMID: 25742744
  24. TGFB3 polymorphism, significant differences were observed for allele and genotype frequencies between caries free and caries affected individuals in oral cleft group (p = 0.013 and 0.006 for allele and genotype frequencies respectively). PMID: 25307808
  25. TGF-beta3 may contribute to the persistent intestinal dysfunction seen in gastroschisis-related intestinal dysfunction. PMID: 25431043
  26. Data indicate greater staining for transforming growth factor beta 3 (TGF-beta3) in healthier tissue compared with diseased menisci. PMID: 25418724
  27. The results show an intricate interplay between p53 and TGF-beta3 whereby p53 inhibits the TGF-beta3-induced expression of genes, e.g., EPHB2, to impede tumor cell invasion and migration PMID: 25257729
  28. findings emphasize the broad clinical variability associated with TGFB3 mutations and highlight the importance of early recognition of the disease because of high cardiovascular risk PMID: 25835445
  29. It was found that the majority of TGF-beta3 that was loaded onto the scaffold was released in a controlled manner over the first 10days of culture, with comparable long-term chondrogenesis PMID: 24907658
  30. We identified genetic variants in TGFB3 and ARHGAP29 associated with suboptimal healing outcome. PMID: 24635173
  31. These results unequivocally demonstrate that human TGF-beta3 elicits bone induction by up-regulation of endogenous BMP-2 and is blocked by hNoggin. PMID: 24438909
  32. TGF-beta3 could be stably expressed in pcDNA3.1(+)-hTGF-beta3-transfected PSCs. PMID: 21823016
  33. Liarozole decreased TGF-beta3 and TGF-beta3-mediated extracellular matrix expression in a 3D uterine leiomyoma culture system. PMID: 24825427
  34. meta-analysis suggests that TGF-beta3 gene polymorphisms may contribute to NSCLP susceptibility, especially among Asian populations. PMID: 24053560
  35. Allelic and haplotypic associations implicate a possible role of TGFB3 in nonsyndromic cleft lip with or without cleft palate in the Chilean population. PMID: 20170386
  36. This study provides a comprehensive list of genes differentially expressed in the healing corneal epithelial cells of diabetic corneas and suggests the therapeutic potential of TGF-beta3 for treating corneal and skin wounds in diabetic patients. PMID: 24306208
  37. The GG genotype and G allele of TGF-beta 3 were significantly different in the patient group compared with the control. PMID: 23023602
  38. Shear stress of vascular endothelial cells induces TGF-beta3 signaling and subsequent activation of Kruppel-like factor 2 and NO, and represents a novel role for TGF-beta3 in the maintenance of homeostasis in a hemodynamic environment. PMID: 23968981
  39. Mutation in TGFB3 is associated with a syndrome of low muscle mass, growth retardation, distal arthrogryposis and clinical features overlapping with Marfan and Loeys-Dietz syndrome. PMID: 23824657
  40. These findings suggest human serum, FGF-2 and TGF-beta3 as possible candidates to support biological treatment strategies of AF defects. PMID: 23122986
  41. These results highlight TGFbeta/5-HT signaling as a potent mechanism for control of biomechanical remodeling of atrioventricular cushions during development. PMID: 22880017
  42. Hypoxia may inhibit the invasion of human extravillous trophoblast cells through inducing the integrin switch from alpha1 integrin to alpha5 integrin and promoting TGFB3 expression. PMID: 22674391
  43. IL-1B & TGFB3 synergistically activated MMP)-1, MMP-3,& MMP-10 gene expression in NSCLC cells via MAPK-dependent pathways. PMID: 22796605
  44. Significant statistical differences were found for genotype frequencies between tooth agenesis and TGFB3 control samples, as well as for allele and genotype frequencies between unilateral tooth agenesis and TGFB3 control samples. PMID: 22191848
  45. TGF-beta3 isoform is a key agent in seminal plasma that signals induction of proinflammatory cytokine synthesis in cervical cells. PMID: 22706080
  46. analysis of genetic variants hints at the contribution of TGFB3 and MN1 in the aetiology of submucous cleft palate PMID: 22409215
  47. involved in the modulation of epithelial barrier function by regulating assembly of tight junctions PMID: 22369552
  48. This study confirms the crucial role of TGF-beta3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-beta3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation PMID: 22143699
  49. We observed significant downregulation of Transforming Growth Factor beta 3 in women with recurrent miscarriage compared with controls. PMID: 22266274
  50. study suggests that both combination medium, but better with the addition of TGF-ss3 could enhance BMSCs chondrogenesis in vivo, and promotes themaintenance of the chondrocyte phenotype PMID: 21666950

Show More

Hide All

Involvement in disease Arrhythmogenic right ventricular dysplasia, familial, 1 (ARVD1); Loeys-Dietz syndrome 5 (LDS5)
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

HGNC: 11769

OMIM: 107970

KEGG: hsa:7043

STRING: 9606.ENSP00000238682

UniGene: Hs.592317

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1