Human Transforming Growth factor β3,TGF-β3 ELISA kit

Code CSB-E13738h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P10600
Lead Time 3-5 working days
Abbreviation TGFB3
Target Name transforming growth factor, beta 3
Alias ARVD, FLJ16571, TGF-beta3,
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1.
HGNC 11769
RGD 3851
MGI 98727
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-95
1:2Average %97
Range %94-103
1:4Average %98
Range %90-104
1:8Average %87
Range %82-91
Recovery
The recovery of human TGF-β3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-98
EDTA plasma (n=4)9896-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.699 2.752 2.726 2.571
5002.435 2.399 2.417 2.262
2501.808 1.875 1.842 1.687
1251.065 1.055 1.060 0.905
62.50.678 0.735 0.707 0.552
31.20.401 0.422 0.412 0.257
15.60.267 0.275 0.271 0.116
00.149 0.160 0.155  
References
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Function Involved in embryogenesis and cell differentiation.
Involvement in disease Arrhythmogenic right ventricular dysplasia, familial, 1 (ARVD1); Loeys-Dietz syndrome 5 (LDS5)
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

HGNC: 11769

OMIM: 107970

KEGG: hsa:7043

STRING: 9606.ENSP00000238682

UniGene: Hs.592317

Pathway Hippo signaling pathway
MAPK signaling pathway
TGF-beta signaling pathway
Cell cycle
Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway

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