TGFB3 Antibody

Datasheet
Code CSB-PA17749A0Rb
See More Details Free Antibody trial simple
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Uniprot No. P10600
Image
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA17749A0Rb at dilution of 1:100

  • IHC image of CSB-PA17749A0Rb diluted at 1:450 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Recombinant Human Transforming growth factor beta-3 proprotein protein (301-412AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:50-1:500
Relevance Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.Latency-associated peptide: Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity). Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity). Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Transforming growth factor beta-3 (TGF-beta-3) [Cleaved into: Latency-associated peptide (LAP)], TGFB3
Immunogen Species Human
Research Area Signal Transduction
Target Names TGFB3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in embryogenesis and cell differentiation.
Involvement in disease Arrhythmogenic right ventricular dysplasia, familial, 1 (ARVD1); Loeys-Dietz syndrome 5 (LDS5)
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

HGNC: 11769

OMIM: 107970

KEGG: hsa:7043

STRING: 9606.ENSP00000238682

UniGene: Hs.592317

Pathway Hippo signaling pathway
MAPK signaling pathway
TGF-beta signaling pathway
Cell cycle
Cellular senescence
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway

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