| Code | CSB-E04655h |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The human CCL2 ELISA Kit quantitates human CCL2 levels in multiple samples, including serum, plasma, urine, saliva, cerebrospinal fluid (CSF), ascitic fluid, cell culture supernates, and tissue homogenates. CCL2, also called MCP-1, is inducibly expressed when exposed to inflammatory stimuli, such as IL-1, IL-4, IL-6, and TNFα. CCL2-CCR2 signaling activates several downstream signaling cascades, including JAK2/STAT3, MAPK, and PI3K pathways. CCL2-CCR2 interaction is involved in neurogenesis, neurotransmission, neuroinflammation, neurodegeneration, neuroregeneration, and other neurobiological processes. CCL2 is implicated in the pathogenesis of diseases, including congestive heart failure, multiple sclerosis, and atherosclerosis. Besides, CCL2 has been found in many types of cancer, such as breast, prostate, and pancreatic cancer, where CCL2 not only recruits immune cells to the tumor microenvironment but also acts on tumor cells to regulate angiogenesis, metastasis, and tumor cell proliferation. This kit employs the sandwich-ELISA mechanism in conjugation with CCL2 antibody-CCL2 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of CCL2 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%. |
| Alternative Names | C-C motif chemokine 2 ELISA Kit; CCL2 ELISA Kit; CCL2_HUMAN ELISA Kit; Chemokine (C C motif) ligand 2 ELISA Kit; GDCF 2 ELISA Kit; GDCF-2 ELISA Kit; GDCF2 ELISA Kit; HC11 ELISA Kit; HSMCR30 ELISA Kit; JE ELISA Kit; MCAF ELISA Kit; MCP 1 ELISA Kit; MCP-1 ELISA Kit; MCP1 ELISA Kit; MGC9434 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic and activating factor ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; Monocyte secretory protein JE ELISA Kit; SCYA2 ELISA Kit; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je) ELISA Kit; Small inducible cytokine A2 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 2 ELISA Kit; Small-inducible cytokine A2 ELISA Kit; SMC CF ELISA Kit; SMC-CF ELISA Kit; SMCCF ELISA Kit |
| Abbreviation | CCL2 |
| Uniprot No. | P13500 |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, urine, saliva, cerebrospinal fluid (CSF), ascitic fluid, cell culture supernates, tissue homogenates |
| Detection Range | 6.25 pg/ml -400 pg/ml |
| Sensitivity | 1.56 pg/ml |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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| Gene References into Functions |
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| Subcellular Location | Secreted. |
| Protein Families | Intercrine beta (chemokine CC) family |
| Tissue Specificity | Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes. |
| Database Links |
HGNC: 10618 OMIM: 158105 KEGG: hsa:6347 STRING: 9606.ENSP00000225831 UniGene: Hs.303649 |
