Code | CSB-E11747h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human MyD88 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 87 | ||||
Range % | 82-93 | |||||
1:2 | Average % | 100 | ||||
Range % | 97-104 | |||||
1:4 | Average % | 99 | ||||
Range % | 96-102 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 |
The recovery of human MyD88 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 85 | 81-90 | ||||
EDTA plasma (n=4) | 92 | 88-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human MYD88 ELISA Kit was designed for the quantitative measurement of Human MYD88 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312ng/mL-20ng/mL and the sensitivity is 0.078 ng/mL.
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I will be doing cell culture MDA-MB-231 and would like to test on MyD88 expression of the cells after treatment. I would like to know, should I use cell lysate or cell culture supernatant/medium?
Regarding the information on MYD88, you could refer to this page https://www.uniprot.org/uniprot/Q99836. For this Human MyD88 ELISA Kit (CSB-E11747h), we suggest detecting cell lysate. Due to the distinctiveness of cell samples and the customer's experiment design, we did not test MDA-MB-231. So doing a preliminary experiment at first is recommended.