Code | CSB-E04945m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 90-103 | |||||
1:2 | Average % | 89 | ||||
Range % | 84-95 | |||||
1:4 | Average % | 100 | ||||
Range % | 95-106 | |||||
1:8 | Average % | 97 | ||||
Range % | 92-104 |
The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 93-96 | ||||
EDTA plasma (n=4) | 100 | 95-106 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse IFNB (IFNB) ELISA Kit quantitates mouse IFNB levels in multiple samples, including serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. IFNB is an essential cytokine in promoting and regulating innate and adaptive immune responses. It has both anti-inflammatory and pro-inflammatory properties. IFNB binds to IFNAR1 and IFNAR2 and then activates JAK/STAT pathway, leading to the phosphorylation of STAT1 and STAT2 and the formation of the STAT1/STAT2/IRF3 complex, finally stimulating the expression of genes that encodes proteins exerting antiviral, antiproliferative, and antitumor effects. IFNB has been applied to multiple sclerosis treatment via the downregulation of the MHC class II expression in antigen-presenting cells, the induction of IL-10 synthesis, and the suppression of T cell migration. The antiviral and immunoregulatory effects of IFNB could be most effective if used in the early stages of COVID-19.
This kit employs the sandwich-ELISA mechanism in conjugation with IFNB antibody-IFNB antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of IFNB in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
Sample type: Biological fluids(liver protein lysate)
Sample species: Mouse
Review: ELISA analysis of serum IFN-b levels. Data are expressed as the mean ± SD from at least three independent experiments (n=5).
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Review: Extracellular stimulation of WRS elicits innate immune responses. (A) ELISA of IFN- (left) and IL-6 (right) levels in the supernatant of THP-1 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng) was used as a positive control. (B) ELISA of IFN- (upper left), IL-6 (upper right), IFN- (lower left), and TNF- (lower right) levels in the supernatant of Raw264.7 cells treated with the indicated dose of rWRS for 12 or 24 h. LPS (100 ng/ml) was used as a positive control.
By Anonymous
Sample type: Serum
Sample species: Mouse
Sample dilution: 1:10
Review: I used CSB-E04945m to test the serum IFN-β level in mice, the kit has sufficient samples, the color correspondence between samples and dilutions is intuitive, the instructions are concise and clear, the operation is easy to follow, the data is accurate and satisfactory.
By Anonymous