Mouse Interleukin 1β,IL-1β ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Associated names

Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit

Abbreviation

IL1B

Uniprot No.

P10749

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell culture supernates

Detection Range

12.5 pg/ml-800 pg/ml

Sensitivity

3.125 pg/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-109
1:2	Average %	97
	Range %	91-104
1:4	Average %	98
	Range %	93-103
1:8	Average %	93
	Range %	86-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

800

2.928

2.734

2.831

2.652

400

1.978

1.967

1.973

1.794

200

1.198

1.128

1.163

0.984

100

0.684

0.699

0.692

0.513

0.504

0.521

0.513

0.334

0.392

0.373

0.383

0.204

12.5

0.315

0.298

0.306

0.128

0.183

0.175

0.179

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This kit provides quantitative measurement of interleukin-1β (IL-1β) in mouse samples, supporting research into innate immune activation, inflammasome signaling, and pyroptic inflammatory responses. Because IL-1β is often measured across both systemic and local tissue compartments to track NLRP3 inflammasome-driven inflammation, the kit is designed to accommodate diverse sample contexts within a single assay platform.

Detection Range: The 12.5–800 pg/ml range spans the concentrations typically observed in mouse serum and plasma during LPS challenge, sterile injury, or infection models, where circulating IL-1β can rise from near-undetectable baseline levels to hundreds of pg/ml. Most stimulated samples can be run neat or with minimal dilution, simplifying assay planning.

Sensitivity: At 3.125 pg/ml, the kit detects the low basal IL-1β levels present in unstimulated controls, naïve serum, or early time points following inflammasome priming—conditions where IL-1β concentrations often fall below 15 pg/ml.

Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants enables researchers to correlate systemic IL-1β levels with local tissue production and in vitro BMDM or dendritic cell stimulation data within a single study.

Assay Efficiency: Requiring only 50–100 µl and 1–5 hours, the protocol accommodates limited-volume samples from retro-orbital bleeds or small tissue biopsies while supporting same-day results across multi-group kinetic experiments.

This kit is directly applicable to studies of sepsis modeling, NLRP3 inflammasome activation, and colitis-associated mucosal inflammation.

Related Products

Target Background

Function
(From Uniprot)

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

Gene References into Functions

propofol was the most potent inhibitor of IL-1beta secretion and ROS level in S. aureus-infected RAW264.7 cells; moreover, propofol resulted in an increase in bacterial survival by inhibiting ROS and phagocytosis. PMID: 29667111
P7 is an intracellular proton-gated H +-channel of the hepatitis C virus . P7 activity induced production of interleukin IL-1beta in liver macrophages. PMID: 27979709
These results indicate that in RGCs, ANXA1 increases IL-1beta expression by recruiting p65 to the nucleus, which induces cell apoptosis. The obtained results may help the development of a novel treatment strategy against RGCs apoptosis in acute ischemia-reperfusion injury. PMID: 28389361
Macrophage-derived IL1B/NF-kappaB signaling mediates parenteral nutrition-associated cholestasis in a mouse model. PMID: 29643332
fenretinide impaired proinflammatory cytokine interleukin 1 beta (IL-1beta) production in response to A. fumigatus exposure with contributions by lectin-type oxidized LDL receptor 1 (LOX-1) and c-Jun N-terminal kinase (JNK). PMID: 30211745
observation from the present research work reveals that Quercetin suppressed the production of proinflammatory cytokines at different levels, such as TNF-alpha and IL-1beta, and inhibits the activation of I-kappaB phosphorylation, whereas the total content was not affected. PMID: 29322353
Overall, the authors showed that CCN1 increased IL-1beta production via p38 MAPK signaling, indicating a role for CCN1 protein in regulating inflammation in psoriasis. PMID: 28266627
In fibrocystin/polyductin complex-defective cholangiocytes, beta-catenin and IL-1beta are responsible for signal transducer and activator of transcription 3-dependent secretion of CXCL10 PMID: 29140564
These results suggest that mitochondrial ROS-TXNIP/NLRP3/IL-1beta axis activation is responsible for tubular oxidative injury, which can be ameliorated by MitoQ via the inhibition of mtROS overproduction PMID: 29475133
Circadian clock protein BMAL1 regulates IL-1beta in macrophages via NRF2. PMID: 30127006
TLR2 and NLRP3 inflammasome activation in cardiac macrophages mediate the production of IL-1beta in diabetic mice. IL-1beta causes prolongation of the action potential duration, induces a decrease in potassium current and an increase in calcium sparks in cardiomyocytes, which are changes that underlie arrhythmia propensity. PMID: 27882934
show that mutant KRAS facilitates IKKalpha-mediated responsiveness of tumor cells to host IL-1beta, thereby establishing a host-to-tumor signaling circuit that culminates in inflammatory MPE development and drug resistance PMID: 29445180
identify interleukin-1 beta as an upstream trigger for the upregulation of interactions between USP5 and Cav3.2 channels in the pain pathway PMID: 28741432
The present study demonstrated that IL-1b may induce ICAM-1 expression, thus enhancing the cohesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. PMID: 29393395
SAG2A differentially modulates IL-1beta expression in resistant and susceptible murine peritoneal macrophages cells. PMID: 29353306
High IL-1beta expression is associated with experimental autoimmune encephalomyelitis. PMID: 29358392
tibias of botulin A toxin-treated and tail-suspended mice, which featured unloading and decreased bone mass, showed higher expression of IL-1beta, Lcn2 and Nos2, suggesting their pathophysiologic involvement in endothelial cell-osteoblast crosstalk. PMID: 27430980
HMGB1/IL-1beta complexes released after burn injuries can modulate immune responses PMID: 29601597
bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1beta and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1beta from pro-IL-1beta. PMID: 28766178
The present study demonstrates a novel mechanism underlying LPS-induced innate immunity; that is, a secondary upregulation of IL-1beta-IL-1RI signaling is responsible for alveolar macrophages pyroptosis and augmented lung injury in response to LPS. PMID: 27526865
IL-33 may induce Th17 cell responses via IL-1beta and IL-6 derived from IL-33-matured dendritic cells. PMID: 28802996
ESP of fifth-stage larval Angiostrongylus cantonensis stimulates astrocyte activation and IL-1beta and IL-6 production through NF-kappaB and the Shh signaling pathway. PMID: 28950910
we confirmed that Th1 cell-conditioned medium decreased Cx43 protein levels in mixed glial cell cultures. These findings suggest that Th1 cell-derived IFNg activates microglia to release IL-1b that reduces Cx43 gap junctions in astrocytes. Thus, Th1-dominant inflammatory states disrupt astrocytic intercellular communication and may exacerbate multiple sclerosis. PMID: 27929069
This finding shows that autophagy and NLRP3 inflammasome activation are connected, and that PTPN22 plays a key role in the regulation of those 2 pathways. PMID: 28786745
These data suggest that amyloid formation leads to reduced PKB phosphorylation in beta-cells which is associated with elevated islet IL-1beta levels. Inhibitors of amyloid or amyloid-induced IL-1beta production may provide a new approach to restore phospho-PKB levels thereby enhance beta-cell survival and proliferation in conditions associated with islet amyloid formation PMID: 29474443
Mice treated with HW for 4 weeks demonstrated a significant decrease in the AD severity score compared with PW-treated mice (p less than 0.01). Hydrogen water administration also significantly reduced TEWL and serum TARC levels (p less than 0.01), infiltration of mast cells (p less than 0.05), and secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-33 (p less than 0.05) in skin lesions compared wit... PMID: 28889151
Curcumin attenuated neuropathic pain and down-regulated the production of spinal mature IL-1beta by inhibiting the aggregation of NALP1 inflammasome and the activation of the JAK2-STAT3 cascade in astrocytes. PMID: 27381056
results altogether demonstrate distinct roles of SHARPIN in initiating systemic inflammation and dermatitis. Furthermore, skin inflammation in Sharpin(cpdm) mice is specifically modulated by IL-1beta, highlighting the importance of specific targeted therapies in the IL-1 signaling blockade. PMID: 27892465
Food-grade synthetic amorphous silica particles are able to directly initiate the endosomal MyD88-dependent pathogen pattern recognition and signaling pathway in steady-state dendritic cells. The ensuing activation of immature DCs results in de novo induction of pro-IL-1beta. PMID: 28645296
the present study confirmed that miR98 targeted the 3'untranslated region of Bcl2. In conclusion, miRNAcoordinated regulation of apoptosisassociated protein expression has been identified in Osteoarthritis chondrocytes following IL1beta induction. PMID: 28765925
Results provide evidence that IL-1beta does not contribute to the pathophysiology of doxorubicin-induced cardiotoxicity. PMID: 27225830
Alendronate (ALN)-augmented IL-1beta production and cell death require Smad3 and ASC activation, and SIS3 and anti-ASC antibodies may serve as palliative agents for necrotizing inflammatory diseases caused by ALN PMID: 29438662
urinary LRG is produced in renal tubular epithelial cells by interleukin-1beta (IL-1beta) that is released during proteinuria-induced renal damage PMID: 29550485
These data reveal how, upon XIAP deficiency, a TLR-TNF-TNFR2 axis drives cIAP1-TRAF2 degradation to allow TLR or TNFR1 activation of RIPK3-caspase-8 and IL-1beta. This mechanism may explain why XIAP-deficient patients can exhibit symptoms reminiscent of patients with activating inflammasome mutations. PMID: 28723569
IL-1beta exerts variable effects on long-term potentiation at different kinds of synapses, indicating that IL-1beta has synapse-specific effects on hippocampal synaptic plasticity. PMID: 28637953
we assessed the role of RIP3 in synergy with Caspase-1 in the induction of IL-1beta production in BMDM after either LPS/ATP or Chlamydia muridarum stimulation. The possibility of pyroptosis and necroptosis interplays and the role of RIP3 in IL-1beta production during Chlamydia muridarum infection in BMDM was investigated as well. PMID: 28660207
Inhibition of signaling stimulated by both TNF and IL1beta synergizes with NF-kappaB inhibition in eliminating leukemic stem cells. PMID: 28039479
Parenchymal polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), have a positive correlation with IL1a, IL8, CXCL5, and Mip-1a, suggesting they may attract PMN-MDSC into the tumor PMID: 27799249
Chemokine receptor 2 (CCR2(+)) monocytes invade the hippocampus between 1 and 3 d after SE. In contrast, only an occasional CD3(+) T lymphocyte was encountered 3 d after SE. The initial cellular sources of the chemokine CCL2, a ligand for CCR2, included perivascular macrophages and microglia. The induction of the proinflammatory cytokine IL-1beta was greater in FACS-isolated microglia than in brain-invading monocytes PMID: 27601660
hypernociception in experimental model of autoimmune encephalomyelitis may be a consequence of the increase in some cytokines in dorsal root ganglia, especially IL-1beta. PMID: 26614512
An OA model was established in mouse articular chondrocytes (MACs) treated by interleukin-1beta (IL-1beta). PMID: 29247798
The current study demonstrated that honey can stimulate or suppress the mRNA expression of some pro-inflammatory cytokines in mice brains. Furthermore, honey suppresses the TNF-alpha mRNA expression in the presence of T. gondii infection but it stimulates the IL-1beta and IL-6 mRNA expression. Treatment of the mice with honey reduces parasite multiplication in the brain. PMID: 27591508
IL-1beta has a direct effect on NGAL production by tubular epithelial cells. PMID: 27997859
Elevations of CO2 cause oligomerization of the inflammasome components ASC, NLRP3, caspase 1, thioredoxin interacting protein, and calreticulin - a protein from endoplasmic reticulum, leading to IL-1beta synthesis. An increased production rate of MPs containing elevated amounts of IL-1beta persists for hours after short-term exposures to elevated CO2 PMID: 28288918
dimerized or endogenous caspase-8 can also directly cleave IL-1beta into its biologically active form, in the absence of canonical inflammasome components. PMID: 27419363
In this newborn mouse lung hypoxia-reoxygenation model, we found downregulation of genes of mediators of inflammation, an antiapoptotic gene expression pattern, and downregulation of DNA glycosylases. Sod1 and Il1b were significantly differentially expressed when comparing reoxygenation using 60% O2 with air. PMID: 27529351
Report direct role of pleural cells in the pathogenesis of bleomycin-induced pulmonary fibrosis via caspase-1/IL-1beta pathway. PMID: 27894300
the senescence associated secretory phenotype was also increased significantly in the kidney of Sod1(-/)(-) mice compared to WT mice as measured by the expression of transcripts for IL-6 and IL-1b PMID: 27846439
These studies elucidate an important role for neutrophils and IL-1beta in lung carcinogenesis. PMID: 27320908
PLCd1 negatively regulates lipopolysaccharide-induced production of IL-1b and Fc gamma receptor-mediated phagocytosis in macrophages. PMID: 26643908

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Subcellular Location

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.

Protein Families

IL-1 family

Tissue Specificity

Expressed in activated macrophages (at protein level).

Database Links

KEGG: mmu:16176

STRING: 10090.ENSMUSP00000028881

UniGene: Mm.222830

Code	CSB-E08054m-IS
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.