Mouse cyclooxygenase-2,COX-2 ELISA Kit

Instructions
Code CSB-E12910m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Alternative Names Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Pghs-b ELISA Kit; Tis10 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; Glucocorticoid-regulated inflammatory cyclooxygenase ELISA Kit; Gripghs ELISA Kit; Macrophage activation-associated marker protein P71/73 ELISA Kit; PES-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit; TIS10 protein ELISA Kit
Abbreviation PTGS2
Uniprot No. Q05769
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-92
1:2Average %98
Range %91-105
1:4Average %100
Range %92-110
1:8Average %93
Range %86-98
Recovery
The recovery of mouse COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.660 2.654 2.657 2.547
10002.107 2.046 1.994 1.884
5001.494 1.385 1.440 1.330
2500.932 0.911 0.922 0.812
1250.502 0.489 0.496 0.386
62.50.403 0.398 0.415 0.305
31.250.223 0.207 0.215 0.105
00.114 0.106 0.110  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Gene References into Functions
  1. These results suggest a crucial role for the COX-2 signaling pathway in the intermittent hypoxia-exacerbated malignant processes, and designate macrophages and lung adenocarcinoma cells, as potential sources of prostaglandin E2. PMID: 28300223
  2. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in Alzheimer's disease patients and in a transgenic mouse model. PMID: 29662056
  3. Results demonstrate that neither the basal nor seizure-induced expression profiles of COX-2 were altered in mice lacking a functional TIA-1 gene suggesting that TIA-1 does not contribute to regulation of COX-2 protein expression in neurons. Induced seizure threshold was also unchanged in mice lacking TIA-1 protein, indicating that this RNA binding protein does not influence the innate seizure threshold. PMID: 29337236
  4. The kidney is the principle site in the body where local COX-2 controls blood flow via PPARbeta/delta-mediated renal vasodilator pathway. PMID: 29295852
  5. COX-2 is an important factor for Dengue virus replication. PMID: 28317866
  6. The COX2-dependent lipid inflammatory pathway is responsible for lethality in F. novicida infection due to overproduction of proinflammatory effectors including prostaglandin E2. PMID: 29109289
  7. PTGS2 deletion changes the natural distribution of ANXA2 in monocytes/macrophages, increasing TF expression and activity predisposing to venous thrombosis. PMID: 28536720
  8. Study suggest that amyloid beta-protein increase the expression of TRPC6 via NF-kappaB in BV-2 microglia and promotes the production of COX-2, which induces hippocampus neuron damage. PMID: 28458019
  9. Data show that patients with high cyclooxygenase-2 (COX2) gene expression who received celecoxib had a significantly higherpathological complete response (pCR) rate compared with patients with low COX2 gene expression. PMID: 29491076
  10. COX-2/mPGES-1/PGE2 cascade activation mediates uric acid-induced glomerular mesangial cell proliferation. PMID: 28052039
  11. Cobalt protoporphyrin induces COX-2 expression through activating P2X7 receptors and ASK1/MAP kinases as well as PIAS1 degradation signaling pathways. PMID: 26255181
  12. sUV activated the transcription factors nuclear factor-kappaB and activator protein-1 which, in turn, induces COX-2 expression. PMID: 28409880
  13. Results indicate that Cox-2 promotes Col10a1 expression and chondrocyte hypertrophy in vitro. PMID: 27121205
  14. These data reveal important structure-function and signaling differences between the two FFA4 isoforms, and for the first time link FFA4 to modulation of ROS in macrophages. PMID: 28943238
  15. Our data suggest that there are physiologically important gender differences in hypoxic acclimatization in COX-2-deficient mice. The COX-2 signaling pathway appears to be required for acclimatization in oxygen-limiting environments only in males, whereas female COX-2-deficient mice may be able to access COX-2-independent mechanisms to achieve hypoxic acclimatization. PMID: 28242826
  16. increased COX2 expression has an impact on the aging process PMID: 27750221
  17. Angiotensin II-AT1-receptor signaling is necessary for COX-2-dependent normal postnatal nephrogenesis and maturation. PMID: 28040266
  18. AhR controls COX-2 protein via mRNA stability. PMID: 28749959
  19. Podocyte-specific knockout of COX2 enhanced albuminuria and did not attenuate the histologic features of diabetic kidney disease. PMID: 28490532
  20. Salt supplementation during the COX-2-dependent time frame of nephrogenesis partly reverses renal morphological defects in COX-2(-/-) mice and improves kidney function. PMID: 28274925
  21. Data suggest that induction of Ptgs2 expression in preimplantation uterus may be earliest positive embryo/blastocyst signal for implantation and pregnancy recognition in mice. PMID: 28215431
  22. the bone regeneration capacity of Cox-2KO MDSCs was impaired because of a reduction in cell proliferation and survival capacities, reduction in osteogenic differentiation and a decrease in the ability of the cells to recruit host cells to the injury site. PMID: 27354351
  23. Data (including data from studies using knockout/mutant mice) suggest that Mir200c (microRNA 200c) is involved in endothelial function/dysfunction via regulation of Cox2 (cyclooxygenase-2) expression; overexpression of Mir200c impairs endothelium-dependent vascular relaxation (EDVR) in non-diabetic mouse aorta, whereas suppression of Mir200c by anti-Mir200c enhances EDVR in diabetic mouse aorta. PMID: 26822089
  24. data indicate that excessive adipocyte lipolysis activates the JNK/NFkappaB pathway leading to the up-regulation of COX-2 expression and recruitment of inflammatory macrophages. PMID: 27246851
  25. results suggest that Cox-2 is involved in the pathogenesis of noise-induced hearing loss; and pharmacological inhibition of Cox-2 has considerable therapeutic potential in noise-induced hearing loss. PMID: 26934825
  26. COX-2 and EP1 receptors participate in the increased extracellular matrix deposition and vascular stiffness, the impaired vascular function and inflammation in hypertension. Targeting PGE2 receptors might have benefits in hypertension-associated vascular damage. PMID: 26856544
  27. these results not only provide a dataset of protein expression change in FA treatment but also suggest that Cox-2 and lipid droplets (LDs) are potential players in PA- and OA-mediated cellular processes PMID: 26899878
  28. prostaglandin E2 (PGE2) as a damage-associated molecular pattern that negatively regulates immune responses. The production of PGE2 is augmented under cell death-inducing conditions via the transcriptional induction of the cyclooxygenase 2 gene and cell-released PGE2 suppresses the expression of genes associated with inflammation, thereby limiting the cell's immunostimulatory activities. PMID: 27001836
  29. DHA and celecoxib diminished the COX-2 and iNOS expression in the cells. This was associated with increased PPARgamma activity, supressed NF-kappaB activity in the nucleus. PMID: 26954392
  30. Tat-SOD inhibited SNP-induced COX-2 expression similarly to celecoxib and prevented the formation of peroxynitrite as 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. PMID: 26786970
  31. Glucose-related hyperosmolarity seems to be able to promote angiogenesis and retinopathy through activation of TonEBP and possibly increasing expression of AQP1 and COX-2. PMID: 26822858
  32. COX-2 has a direct role in modulating tumor progression in tumors arising within collagen-dense microenvironments, and suggest that COX-2 may be an effective therapeutic target for women with dense breast tissue and early-stage breast cancer. PMID: 27000374
  33. COX-2 deletion delays BM megakaryopoiesis promoting compensatory splenic MK hyperplasia, releasing hyper-responsive platelets increasing thrombogenicity. COX-2 contributes to MK maturation and pro-platelet formation. PMID: 26272103
  34. Ptgs2 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
  35. Modulation of COX-2-driven metabolization of 2-AG may provide a novel physiological concept allowing the specific targeting of HSCs in liver fibrosis. PMID: 26801558
  36. alveolar type II cell-derived COX-2 plays an important role in regulating basal airway function and LPS-induced lung inflammation PMID: 26396235
  37. COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast. PMID: 26066979
  38. The ERK5 pathway is essential in the induction of COX-2 gene. PMID: 25976667
  39. 11betaHSD2 inhibition suppressed lung tumor growth and invasion in association with increased tissue active glucocorticoid levels, decreased COX-2 expression, inhibition of ERK and mTOR signaling pathways. PMID: 26011146
  40. COX-2 and EP-2 signaling contribute significantly to the heart leukocyte infiltration and to the release of chemokines and inflammatory cytokines in the heart of T. cruzi infected mice. PMID: 26305786
  41. Results suggest that substrates interact with cyclooxygenase-2 (COX-2) via multiple potential complexes involving binding to both the catalytic and allosteric sites. PMID: 26392530
  42. canolol could inhibit the gastritis-related tumor initiation and progression, and the suppression effect was correlated with the blocking up of canonical COX-2/PGE2 signaling pathway and might be regulated by miR-7. PMID: 25781635
  43. These data suggest that the bone loss with continuously infused PTH in mice is due largely to suppression of bone formation and that this suppression is mediated by Cox2. PMID: 25781979
  44. COX2 may be involved in the expression of HSP47 and type IV collagen through the phosphorylation of ERK and JNK, accelerating renal interstitial fibrosis. PMID: 24975097
  45. SHH-responsive 5-lipoxygenase, 15-lipoxygenase and COX-2 modulate Dectin-1-induced inflammatory cytokines. PMID: 26432261
  46. Co-exposure to arsenic and ethanol increased COX-2 expression in mice. PMID: 26220687
  47. these results demonstrate that during acute inflammation Atf3 negatively regulates Ptgs2 PMID: 25619459
  48. our results reveal a previously unrecognized non-cell-autonomous mechanism in TDP-43-mediated neurodegeneration, identifying COX-2-PGE2 as the molecular events of microglia- but not astrocyte-initiated neurotoxicity PMID: 25811799
  49. Increased endoplasmic reticulum stress in mouse osteocytes with aging alters Cox-2 response to mechanical stimuli PMID: 25539857
  50. data suggests that an as yet unidentified prostaglanind E synthase but not mPGES-1 may couple with COX-2 to mediate increased renal PGE2 sythsesis in DN. PMID: 24984018
  51. data suggest thatincreased COX-2 activity may contribute to proinflammatory responses, including macrophage chemotaxis, during exposure to hyperoxia PMID: 23624331
  52. miR26a/-26b-COX-2-MIP-2 loop regulates allergic inflammation and the feedback relationship between allergic inflammation and the enhanced tumorigenic and metastatic potential PMID: 25907560
  53. cellular cholesterol composition affects biomechanical signaling, which, in turn, affects fluid shear stress-mediated COX-2 expression and Prostaglandin E2 release via a p38-dependent mechanism PMID: 25761882
  54. Action at a distance: mutations of peripheral residues transform rapid reversible inhibitors to slow, tight binders of cyclooxygenase-2. PMID: 25825493
  55. COX2 expression is upregulated in CAVD, and its activity contributes to osteogenic gene induction and valve calcification in vitro and in vivo. PMID: 25722432
  56. In the presence of high HGF, dual inhibition of c-Met and COX-2 may enhance antitumor effects. This combination may have clinical potential in NSCLCs with high HGF/c-Met expression or epithelial-mesenchymal transition phenotype. PMID: 25057941
  57. new insights into how Cyclooxygenase-2 regulates the activation of the pyrin domain-containing 3 (NLRP3) inflammasome and suggest that it may be a new potential therapeutic target in NLRP3 inflammasome-related diseases PMID: 25294243
  58. Following epithelial injury, intestinal myofibroblasts sense innate or inflammatory signals and activate, via Tpl2, the cyclooxygenase-2 (Cox-2)-prostaglandin E2 (PGE2) pathway, which are essential for the epithelial homeostatic response. PMID: 25316791
  59. Upregulation of COX-2 in lung cancer promotes overexpression of multidrug resistance protein 4 via PGE2-dependent pathway PMID: 24909729
  60. data highlight a crucial role for Cox-2 from cells of mesenchymal lineages in modulating key pathways that control periosteal progenitor cell growth, differentiation, and angiogenesis in fracture repair. PMID: 24988184
  61. using inducible RNA interference to target COX-2 expression, we demonstrate potent, reversible Cox2 gene silencing in vivo. PMID: 24988319
  62. High Cyclooxygenase-2 is associated with mammary tumor. PMID: 24590894
  63. The data suggest that local hypertonicity in the medullary thick ascending limb is associated with an increase in COX-2 expression concomitant with elevated EP3 receptor expression, which limits COX-2 activity in this segment of the nephron. PMID: 25080527
  64. upregulation of the COX-2/PGE2 and HGF in macrophages following exposure to apoptotic cells represents a mechanism for mediating the anti-inflammatory and antifibrotic consequences of apoptotic cell recognition PMID: 24959005
  65. Upregulation of COX-2 by ET-1 in microvascular endothelial cells is mediated through calcium signaling. PMID: 24287977
  66. Topical COX-2/EP2 treatment reduces CCR7+CD11b+ cells on the ocular surface with inhibition of cellular LN homing and suppresses Th17 immune response PMID: 25257053
  67. Suggest lead-associated inflammatory neurotoxicity occurs via activation of pro-inflammatory NFAT3/COX-2 axis. PMID: 25193092
  68. UV-induced COX-2/PGE2 stimulates beta-catenin signaling, and beta-catenin activation may contribute to skin carcinogenesis. PMID: 24481495
  69. Saturated fatty acids can up-regulate COX-2 expression in prostate epithelial cells, and this effect was mediated mainly through the TLR4/NF-kappaB signaling pathway. PMID: 24221358
  70. Reactive oxygen species increased COX-2 mRNA expression during endometrial breakdown. PMID: 24926822
  71. Concurrent inhibition of 20-HETE and COX-2 can not only enhance anti-tumor efficacy of coxibs, but also prevent coxib-induced adverse stroke events. PMID: 24990856
  72. CREB-dependent expression of COX-2 for the production of antiadipogenic prostaglandins is critical for the regulation of the early phase of adipogenesis. PMID: 24378735
  73. These results demonstrate a critical interaction of these two lipid metabolism pathways on tumorigenesis and suggest dual inhibition of COX-2 and sEH as a potential therapeutic strategy for cancer therapy. PMID: 25024195
  74. The results are consistent with the notion that necrotic and fibrotic lesions are able to increase COX-2 expression in Duchenne muscular dystrophy (DMD). PMID: 23188550
  75. COX-2 inhibition prevents the appearance of cutaneous squamous cell carcinomas accelerated by BRAF inhibitors PMID: 24345644
  76. data suggest that (i) intrinsic COX2 expression in intestinal epithelial cells plays a gender-specific role in tumor development in Apc(Min/+) mice. PMID: 24268915
  77. Conclusively, these results suggest that muramyl dipeptide and staphylococcal lipoteichoic acid cooperatively induce inflammatory response by overproducing COX-2 through NOD2 and TLR2. PMID: 24211871
  78. our data revealed that Cox-2 might play a pivotal role in the accumulation and function of MDSC after traumatic stress. PMID: 24710938
  79. The role of HuR in AhR stabilization of Cox-2 mRNA was confirmed by knockdown of HuR, which resulted in rapid Cox-2 mRNA degradation. PMID: 24086407
  80. intrinsic epithelial COX-2 activity plays a major role in UVB-induced skin cancer, (ii) macrophage/myeloid COX-2 plays no role in UVB-induced skin cancer. PMID: 24469308
  81. Report potency of 2-(2-arylmorpholino)ethyl esters of ibuprofen hydrochlorides as COX-2 inhibitors. PMID: 24243443
  82. These data provide compelling evidence that OPN and COX-2 expressing macrophages are obligatory factors in melanoma growth. PMID: 23728342
  83. Postnatal global deletion of COX-2 accelerates atherogenesis in hyperlipidemic mice, a process delayed by selective enzyme deletion in macrophages. PMID: 24519928
  84. RelB/microRNA-146a axis attenuates exaggerated COX-2 expression in response to cigarette smoke in lung fibroblasts. PMID: 24472607
  85. Investigated the roles of COX-2, TNF-alpha, IL-6 in the pathogenesis of autoimmune-type recurrent spontaneous abortion. The expression of COX-2 in autoimmune-type recurrent spontaneous abortion was significantly lesser than in normal pregnant models. PMID: 24144034
  86. Data suggest that nicotine impairs mucosa-dependent Bdkrb2 (bradykinin B2 receptor) mediated trachea relaxation; nicotine also down-regulates tracheal expression of COX2/Ptgs2 (cyclooxygenase 2) and mPGES-1 (microsomal prostaglandin E2 synthase-1). PMID: 24380835
  87. Airway fibrosis after exposure to allergen and MWCNTs was no different between WT and COX-2(-/-) mice. PMID: 23642096
  88. Data suggest expression of Ptgs2/Ptger2 (prostaglandin E receptor 2) is induced in cumulus cells of females sired by males with Y-chromosome long-arm deletion; thus, paternal genes on Y-chromosome are involved indirectly in female reproduction. PMID: 22953728
  89. Cav-1 may be a cofactor in the interaction of Derlin-1 and N-glycosylated COX-2 and may facilitate Derlin-1- and p97 complex-mediated COX-2 ubiquitination, retrotranslocation, and degradation. PMID: 24089527
  90. Dihydroartemisinin inhibited phorbol 12-myristat e 13-acetate (PMA)-mediated Prostaglandin E2 (PGE2) production and COX-2 expression in murine macrophages. PMID: 23429041
  91. Netrin-1 regulates the inflammatory response of neutrophils and macrophages through suppression of COX-2-mediated PGE2 production in acute ischemic kidney injury. PMID: 23447066
  92. Up-regulation of COX-2/PGE2 and HGF through a positive-feedback loop may be an important mechanism whereby apoptotic cell instillation exerts the net results of anti-inflammatory, antiapoptotic, and antifibrotic action. PMID: 23922381
  93. During brain development COX2 expression is upregulated and glycosylated in an age-dependent manner. PMID: 24005111
  94. these findings suggest that Hcy enhances COX-2 expression in murine macrophages by ROS generated via NMDA receptor-mediated calcium signaling pathways PMID: 23485152
  95. Cox-2 expression is regulated by the androgen/androgen receptor pathway. PMID: 23415714
  96. COX-2-derived PGE2 facilitates neointimal hyperplasia response to vascular injury. PMID: 23595951
  97. The phosphatidylinositol (PI)3-kinase/Cdc42/Rac1 axis can be defective in cox2-deficient macrophages, resulting in impaired cell adhesion and migration. PMID: 23733875
  98. AML1-ETO also induces expression of the Cox-2 gene and activates beta-catenin PMID: 23645839
  99. infection of macrophages led to decreased Cox1 and Alox5 expression whereas COX-2 and trans-4-hydroxy-2-nonenal increased PMID: 23321929
  100. COX-2 activity attenuates the changes in nocturnal blood pressure during high salt intake, and COX-2 activity is not necessary for increased renal nitric oxide formation during elevated NaCl intake. PMID: 23535462

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Subcellular Location Microsome membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein
Protein Families Prostaglandin G/H synthase family
Tissue Specificity Following colon injury, expressed in the wound bed mesenchyme during the first phase of repair, probably by colonic mesenchymal stem cells (at protein level).
Database Links

KEGG: mmu:19225

STRING: 10090.ENSMUSP00000035065

UniGene: Mm.292547

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