Mouse cyclooxygenase-2,COX-2 ELISA Kit

Code CSB-E12910m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q05769
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation PTGS2
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Alias COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, cyclooxygenase 2b|prostaglandin G/H synthase and cyclooxygenase|prostaglandin-endoperoxide synthase 2
Species Mus musculus (Mouse)
Protein Biological Process 3 Fatty acid biosynthesis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-92
1:2Average %98
Range %91-105
1:4Average %100
Range %92-110
1:8Average %93
Range %86-98
Recovery
The recovery of mouse COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.660 2.654 2.657 2.547
10002.107 2.046 1.994 1.884
5001.494 1.385 1.440 1.330
2500.932 0.911 0.922 0.812
1250.502 0.489 0.496 0.386
62.50.403 0.398 0.415 0.305
31.250.223 0.207 0.215 0.105
00.114 0.106 0.110  
References
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Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Subcellular Location Microsome membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein
Protein Families Prostaglandin G/H synthase family
Tissue Specificity Following colon injury, expressed in the wound bed mesenchyme during the first phase of repair, probably by colonic mesenchymal stem cells (at protein level).
Database Links

KEGG: mmu:19225

STRING: 10090.ENSMUSP00000035065

UniGene: Mm.292547

Pathway VEGF signaling pathway
NF-kappa B signaling pathway
TNF signaling pathway
IL-17 signaling pathway

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