Mouse matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Instructions
Code CSB-E04676m
Size 96T,5×96T,10×96T
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Product Details

Target Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Alternative Names Mmp2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
Abbreviation MMP2
Uniprot No. P33434
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %90
Range %87-103
1:200Average %96
Range %82-105
1:400Average %89
Range %81-99
1:800Average %95
Range %87-108
Recovery
The recovery of mouse MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-105
EDTA plasma (n=4)9584-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.754 2.708 2.731 2.608
5001.843 1.911 1.877 1.754
2501.118 1.074 1.096 0.973
1250.679 0.652 0.666 0.543
62.50.375 0.357 0.366 0.243
31.20.292 0.308 0.300 0.177
15.60.195 0.212 0.204 0.081
00.125 0.121 0.123
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).
Gene References into Functions
  1. In obese mice, periodontitis caused the downregulation of MMP2, and upregulation of TIMP1 and TGF-beta1 at transcriptional and translational levels. PMID: 29322806
  2. In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression PMID: 29267523
  3. Diet and exercise affect atheromatous MMP2/9 activity by modulating the systemic inflammatory milieu, with sVCAM-1, resistin, and adiponectin closely interacting with each other and with visceral fat. PMID: 28883215
  4. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
  5. Aneurysmal-prone factors induced HIF-1alpha can cause overexpression of MMP-2 and MMP-9 and promote aneurysmal progression. PMID: 27363580
  6. These studies illustrated an important role of MMP2 in cognitive and motor behaviors and confirm its importance in NPC activities crucial to brain development, growth and response to and recovery from injury. PMID: 28666838
  7. Secretagogin-dependent MMP2 release from neurons regulates neuroblast migration. PMID: 28223495
  8. This novel mouse model will be a very useful tool for evaluating the mechanistic pathways and for development of novel therapies in cigarette smoke-associated lung emphysema. PMID: 29428733
  9. matrix metalloproteinase 2 (Mmp2) transcript is a target of miR-195a-3p, and that silencing Mmp2 phenocopied the reduced proliferation and migration of MSCs. The therapeutic potential of miR-195a-3p as an angiogenesis inhibitor was also demonstrated in a laser-induced choroidal neovascularization mouse model. PMID: 26989874
  10. developed a novel selective radiolabeled MMP2/9 inhibitor, suitable for single photon emission computed tomography (SPECT) imaging that effectively targets atherosclerotic lesions in mice PMID: 29190653
  11. MMP-2 and MMP-9 have roles in early stages of experimental autoimmune encephalomyelitis induction; MMP-9 from an immune cell source is required in EAE for initial infiltration of leukocytes into the central nervous system PMID: 27831901
  12. This study illustrated that tumour-derived MMP2 has at least two roles in tumour malignancy; to enhance tumour invasiveness by degrading the extracellular matrix and to enhance tumour growth by promoting vessel maturation and function. PMID: 29065106
  13. MMP-2 and -9 expression were suppressed significantly by treatment with SB-3CT. The data demonstrated, for the first time, that SB-3CT strongly reduced corneal lymphangiogenesis and macrophage infiltration during inflammation. PMID: 28669039
  14. animals were submitted to the evaluation of Blood-Brain Barrier permeability and MMP-2 and MMP-9 in striatum, hippocampus and cerebral cortex PMID: 27915985
  15. High MMP2 expression is associated with abdominal aortic aneurysm. PMID: 28179581
  16. Low MMP2 expression is associated with liver fibrosis. PMID: 28118605
  17. Cleavage of beta-DG still occurred when both MMP-2 and MMP-9 were knocked out in gamma - sarcoglycan-deficient mice. The study found that up-regulation of MMP-14 is capable of cleaving beta-DG, and it may be involved in the pathogenesis of sarcoglycanopathy. PMID: 28821434
  18. NH2-terminal truncated MMP-2 "primes" the kidney to enhanced susceptibility to I-R injury via induction of mitochondrial dysfunction. PMID: 28331061
  19. Study demonstrated evidence of beta-dystroglycan cleavage by matrix metalloproteinase-2/-9 in permanent middle cerebral artery occlusion mouse brains; this cleavage was implicated in aquaporin-4 redistribution and brain edema in cerebral ischemia. PMID: 27038751
  20. These results indicate that increased MMP2 and MMP9 activity in the brains of mouse adenovirus type 1-infected susceptible mice may be due to MMP activity produced by endothelial cells, astrocytes, and microglia, which in turn may contribute to blood-brain barrier disruption and encephalitis in susceptible mice. PMID: 28053109
  21. Studies define a novel HMGA1-MMP-2 pathway involved in a subset of human carcinosarcomas and tumor progression in murine models. PMID: 27001612
  22. activation of astrocyte MMP2/JNK1/2 contributes to the pathogenesis of pain hypersensitivity in the complex regional pain syndrome model PMID: 27919822
  23. Ceramide 1-phosphate -stimulated macrophage migration is a receptor mediated effect, and point to MMP-2 and -9 as possible therapeutic targets to control inflammation. PMID: 27164414
  24. MMP-2 potentiates shear-induced platelet activation by enhancing thrombus formation PMID: 26510894
  25. Identify novel MMP-2/cardiac sPLA2 pathway that endows the heart with important endocrine functions, including regulation of inflammation and lipid metabolism in the liver. PMID: 26567374
  26. 129/SvEv mice are more susceptible to abdominal aortic aneurysms compared to C57Bl/6 mice and suggest roles for MMP2/9. PMID: 26546710
  27. Report cross-talk between macrophages, smooth muscle cells, and endothelial cells in response to cigarette smoke alters MMP2/9 levels. PMID: 26318311
  28. MMP-2 silenced hypoxic fibroblasts under hyperglycemic conditions have impaired angiogenic potential. Collagen I/IV secretion is decreased and cell migration is prevented. PMID: 26985676
  29. N-terminal truncated isoform-MMP-2, but not full-length-MMP-2, is the major isoform of MMP-2 involved in skeletal muscle Ischemia-reperfusion injury. PMID: 26213293
  30. These data indicate that oxygen-glucose deprivation-triggered Cav-1 S-nitrosylation interacts with tPA-induced ERK activation to augment MMP2 and 9 secretion and subsequent extracellular matrix degradation. PMID: 26881424
  31. Type IV collagenases, MMP-2 and MMP-9, play important roles in hair cycle, and this could be mediated by induced expression of VEGF, IGF-1, and TGF-beta. PMID: 26451090
  32. Matrix Metalloproteinase-2 Knockout and Heterozygote Mice Are Protected from Hydronephrosis and Kidney Fibrosis after Unilateral Ureteral Obstruction PMID: 26673451
  33. Early MMP-2/MMP-9 activity is not a determinant of long-term recovery after traumatic brain injury in the immature mouse. PMID: 26588471
  34. Taken together, these findings indicate for the first time that AnxA2 phosphorylation and actin remodeling evoked by oxidative stress depend on the sphingolipid pathway, via MMP2 and p38MAPK. PMID: 25574848
  35. Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice PMID: 26355684
  36. OPN might be responsible for vascular remodeling diseases associated with hypertension by increasing MMP-2 production in vascluar smooth muscle cells. PMID: 25986148
  37. Further investigation of MMP2 inhibitors of TIMP2/TIMP4 showed an upregulated TIMP2 expression, but not TIMP4. low-dose pre-radiation attenuates the skin inflammation and ROS production induced by medium-dose UV radiation PMID: 26133107
  38. negatively regulates cardiac secreted phospholipase A2 to modulate inflammation and fever PMID: 25820137
  39. Data show that discoidin domain receptor (DDR) 2 siRNA-mediated suppression of extracellular regulated kinase (ERK) 1 and 2 and nuclear factor of kappa B (NF-kappaB) could down-regulate the expressions of matrix metalloproteinase (MMP) 2 and 9. PMID: 25733533
  40. Findings indicate the importance of MMP-2 in central nervous system development and dendritogenesis, and highlight the importance of a correct developmental wiring for adult brain morphology and function. PMID: 24652381
  41. Dietary supplementation with n-3 PUFAs may have protective anti-inflammatory effects mediated through modulation of MMPs and TIMPs PMID: 25512019
  42. Cytokine-induced MMP-2 activity specifically at the inflammatory border collectively act to accelerate leukocyte chemotaxis across the parenchymal border. PMID: 25704809
  43. MMP2/9 expression and activity are elevated in lacrimal glands of two murine models of Sjogren's syndrome, suggesting that manipulation of MMP2/9 activity might be a potential therapeutic target in chronically inflamed lacrimal glands. PMID: 26244298
  44. Data indicate that gelatinase A (MMP-2) deficient embryonic fibroblasts show reduced differentiation into adipocytes. PMID: 25869489
  45. beta-elemene downregulates expression of uPA, uPAR, MMP-2, and MMP-9 in a murine intraocular melanoma model PMID: 25405459
  46. Smoking equivalent levels of nicotine exposure induces VCAM-1, MMP-2, and MMP-9 expression. PMID: 25381636
  47. PDGF-D intensifies fibrogenesis by interfering with the fibrolytic activity of the TIMP-1/MMP-2/MMP-9 system, and PDGF-D signaling is mediated through both PDGF-alpha and -beta receptors. PMID: 25576870
  48. PPARdelta-mediated modulation of MMP-2 secretion and elastin expression may contribute to the maintenance of skin integrity by inhibiting ROS generation PMID: 25149191
  49. Study reveals the dual role of MMP2 in ECM degradation, as well as ECM synthesis in pathogenesis of thoracic aortic aneurysms. PMID: 25657308
  50. In conclusion, ATRA may increase expression of MMP-2 and MMP-9 by the potential signal pathway of RAR-alpha and RAR-gamma in injury podocyte induced by adriamycin, but not RAR-beta. PMID: 24694005
  51. Reduced beta(2)GP I plays a role in diabetic mice related to vascular protection, inhibiting vascular lipid deposition, and plaque formation by reducing MMPs/TIMPs expression through down-regulation of the p38MAPK signaling pathway. PMID: 25204377
  52. Ivabradine significantly improved cardiac function by attenuating apoptosis and inhibiting the expression and activity of MMP-2 in diabetic mice. PMID: 25361902
  53. the cardiac sterol regulatory element-binding protein-2/3-hydroxy-3-methylglutaryl-coenzyme A reductase pathway being upregulated in MMP-2 deficiency. PMID: 25646300
  54. Suggest role for MMP2 in metastatic outgrowth mediated by fibroblasts, and extend the mechanisms by which MMPs contribute to breast tumour progression. PMID: 25469981
  55. MMP-2 is part of the intracellular proteolytic network in normal skeletal muscle, especially in fast twitch type II fibers. intracellular MMP-2 in skeletal muscle fibers is active during normal homeostasis, and affected by the level of physical activity. PMID: 24905939
  56. mPGES-1 was essential for MMP-2 and MMP-9 up-regulation that enhances tumor metastasis PMID: 24291175
  57. A different spatiotemporal modulation of pro-MMP-2 and pro-MMP-9 activities has been detected in the myocardium during angiogenesis related to the aerobic training PMID: 24195673
  58. MMP-2 regulates neuronal proliferation and cell cycle kinetics in the developing CNS. PMID: 24141049
  59. High mmp2 activity is associated with asthma. PMID: 24508733
  60. Mitochonria associated membrane-localized MMP-2 could therefore potentially impact mitochondrial function by affecting endoplasmic reticulum-mitochondrial Ca(2+) signaling via its proteolysis of calreticulin. PMID: 24375642
  61. Both uPARAP and MMP-2 take part in matrix turnover processes important for bone growth. PMID: 23940733
  62. There exists a degradative Ctsl-MMP-2 axis, resulting in increased MMP-2 levels upon cathepsin deficiency with subsequent degradation of secreted proteins such as collagen alpha-1 (I). PMID: 23811845
  63. the NF-kappaB/Erk1/2 signalling pathway may be key in MMP-2 and MMP-9 production in host defense against toxoplasmosis PMID: 23664424
  64. Data indicate that carbamate 5b and urea 6b show potential for intervention of matrix metalloproteinase-2 (MMP-2)-dependent diseases such as brain metastasis. PMID: 24028490
  65. JAK/STAT3 signaling may play a role in repair process of renal fibrosis in unilateral ureteral obstrauction partly via MMP-2 activation. PMID: 24333535
  66. Whey proteins reduced MMP-2 and pro-MMP-9 expression without pro-MMP-2 in the skin of chronically UVB-irradiated mice. PMID: 24174624
  67. MMP-2 expression is increased in response to high-fat diet intake in C57BL/6J mice yet decreased in SWR/J mice. PMID: 23465590
  68. Report roles of MMP-2/-9 in resolution of venous thrombosis. PMID: 23490298
  69. Enhanced anticancer activity of nanopreparation containing an MMP2-sensitive PEG-drug conjugate and cell-penetrating moiety. PMID: 24062440
  70. In MMP-2(-/-) mice, less prominent proliferation of tubular epithelial cells was evident on days 3 and 7, and damaged tubules that were covered with elongated and immature regenerated epithelial cells were identified on days 7 and 14. PMID: 23548779
  71. results suggest that genetic ablation of tissue inhibitor of matrix metalloproteinase-2 decreases the expression of pro-angiogenic matrix metalloproteinases-2 and increases anti-angiogenic factors resulting in ventricular remodelling and heart failure PMID: 23398532
  72. DNA methylation changes were noted in the Mmp2 gene during chronic cystitis in a murine model. PMID: 23806407
  73. OPN and BSP are known to affect MMP-2 expression and activity but have not previously been shown to be regulated by MMP-2. PMID: 22917927
  74. MMP-2 may contribute to the mechanisms of calcification associated with atherosclerosis PMID: 23312504
  75. renal expression and activity of MMP-2 are increased as a compensatory mechanism in the early phase of diabetic nephropathy PMID: 23028104
  76. Data indicate that in response to phorbol ester-induced inflammation, mice deficient in matrix metalloproteinase 2 (MMP2) showed reduced accumulation of serum proteins in the skin and exhibited different proteolytic networks from those of wild-type mice. PMID: 23322905
  77. mast cell/chymase-mediated intestinal epithelial barrier function is mediated by PAR-2/MMP-2-dependent PMID: 23306080
  78. MMP-2 deficiency and MMP-2 and MMP-9 double deficiency are more protective than MMP-9 deficiency against hemorrhagic transformation after the early stages of ischemia and reperfusion. PMID: 22521821
  79. These results show differential localization, protein expression and enzymatic activation of MMPs, suggesting specific roles for MMP-2 and MMP-9 in decidual and trophoblast tissues related to organogenic ECM remodeling PMID: 22714107
  80. Results show enhanced expression and widespread distribution of MMP-2, MMP-9 and their tissue inhibitors TIMP-1 and TIMP-2 in thymus from infected animals. PMID: 23089194
  81. MMP-2 and MMP-9 act synergistically mainly at the initial step of neutrophil recruitment into the peritoneal cavity, potentiating the action of chemokine CXCL5 during the inflammatory process. PMID: 23225890
  82. CTGF regulates retinal neovascularization through p53 protein-dependent transactivation of the MMP-2 gene. PMID: 23048035
  83. Termination of immunotherapy may result in a higher metastatic potential of residual tumor cells by increasing expression of MMP2 and MMP9. PMID: 18704294
  84. Chuanxiong can ameliorate the age changes in aortic morphology, reduce the production of ROS and AGEs in vascular tissue, inhibit MMP-2 activity and regulate MMP-2/TIMP-2 equilibrium. PMID: 21179735
  85. The changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis. PMID: 20407871
  86. Albumin up-regulated the expression of MMP-2 and MMP-9 at gene and protein levels in a time- and dose-dependent manner in podocytes. PMID: 20037812
  87. Investigated the role of MMP-2 and the effect of inhibition of MMPs on the development of renal fibrosis.The results suggested that MMP-2 have a pathogenic role in renal interstitial fibrosis,possibly through induction of EMT and macrophage infiltration. PMID: 22614125
  88. Higher MMP2 expression in mice on high-fat diet suggests its involvement in the reduction of COL1A1 protein abundance with high-fat diet. PMID: 21775118
  89. A novel N-terminal truncated intracellular isoform of MMP-2 is induced by oxidative stress. PMID: 22509276
  90. Exposure to carbon disulfide disturbs expression of MMP-2 and MMP-9 in embryo and uterus tissues. PMID: 21241594
  91. Increased lipid peroxidation and low-density lipoprotein oxidation in the brain of hyperlipidemic mice are associated with increased activation of matrix metalloproteinase-2/9. PMID: 21836084
  92. Data show that levels of MMP-2 and MMP-9 activity were diminished in the doxycycline (DOX)-treated group. PMID: 22421441
  93. Leukotriene B4 receptor-2 promotes invasiveness and metastasis of ovarian cancer cells through signal transducer and activator of transcription 3 (STAT3)-dependent up-regulation of matrix metalloproteinase 2 PMID: 22396544
  94. MMP-2 and MMP-9 might play a role in the development of cerebral hypoxic preconditioning. PMID: 21158073
  95. The targeted inhibition of MMP-2 and/or TGFbeta would be beneficial for the treatment of bone metastases. PMID: 22238668
  96. Increased MMP-2 expression induced by the interaction of Abeta with RAGE in BECs may contribute to enhanced vascular inflammatory stress in Abeta-related vascular disorders. PMID: 21837459
  97. astrocyte-derived TSP-2 is critical for the maintenance of physiological MMP-2 and MMP-9 levels during the foreign body response and contributes to the repair of the BBB PMID: 21704005
  98. Data suggest that increased activity of MMP-2 and -9 is likely to be a major factor in increased microhemorrhage occurrence. PMID: 21906275
  99. Data suggest that NOS2-derived NO modulates MMP-2 activity and is crucial for an adequate response to and integration of polypropylene mesh implants in the peritoneum. PMID: 21864729
  100. Doxycycline reduced MMP-2 from MEFs and decreased active-MMP-2 from LAM cells. PMID: 21418186

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Subcellular Location Isoform 1: Secreted, extracellular space, extracellular matrix, Membrane, Nucleus
Protein Families Peptidase M10A family
Database Links

KEGG: mmu:17390

STRING: 10090.ENSMUSP00000034187

UniGene: Mm.29564

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