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| Code | CSB-E04676m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 87-103 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 82-105 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 81-99 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 87-108 | |||||
| The recovery of mouse MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-105 | ||||
| EDTA plasma (n=4) | 95 | 84-101 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This mouse MMP2 ELISA Kit is suitable for qualitatively determining mouse concentrations in multiple biological fluids, including mouse serum, plasma, cell culture supernates, and tissue homogenates in vitro.IL27 is a heterodimeric cytokine that plays a role both in innate and adaptive immunity. MMP2 is an enzyme that degrades bone matrix, promotes osteoclastogenesis, and enhances osteolysis in bone metastasis by activating multiple signaling pathways. It plays an essential role in extracellular matrix remodeling and other pathological processes, such as tumor progression and skeletal dysplasia. MMP2 is important for cancer progression, invasion, and metastasis. Excessive activation of MMP2 facilitates osteolytic metastasis and bone destruction in late-stage cancers, while its loss-of-function mutations lead to the declined bone mineralization and generalized osteolysis occurring progressively in skeletal developmental disorders, especially in multicentric osteolysis, nodulosis, and arthropathy (MONA). Either upregulation or downregulation of MMP2 activity can cause the same osteolytic effects.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of mouse MMP2 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-mouse MMP2 antibody. Biotin-labeled MMP2 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the MMP2 in the mouse samples. MMP2 binds to the biotinylated anti-MMP2 mouse monoclonal antibody. And the biotin on the biotinylated anti-MMP2 mouse monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of MMP2 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.
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