Pig C-reactive protein,CRP ELISA Kit

Instructions
Code CSB-E08163p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name C-reactive protein, pentraxin-related
Alternative Names CRP ELISA kit; PTX1C-reactive protein ELISA kit
Abbreviation CRP
Uniprot No. O19062
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 93-100  
1:2 Average % 104  
Range % 101-108  
1:4 Average % 85  
Range % 81-89  
1:8 Average % 93  
Range % 90-96  
Recovery
The recovery of pig CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-91  
EDTA plasma (n=4) 84 81-89  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
4000 2.079 2.098 2.089 1.951  
2000 1.598 1.577 1.588 1.450  
1000 0.934 0.998 0.966 0.828  
500 0.557 0.567 0.562 0.424  
250 0.386 0.387 0.387 0.249  
125 0.283 0.276 0.280 0.142  
62.5 0.201 0.203 0.202 0.064  
0 0.137 0.139 0.138    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Displays several functions associated with host defense
Gene References into Functions
  1. Enhanced external counterpulsation decreased C-reactive protein and increased flow-mediated dilation in hypercholesterolemia. PMID: 22681602
  2. serum C-reactive protein correlates with macrophage accumulation and coronary artery disease in hypercholesterolemic pigs PMID: 12754176
  3. CRP inhibits endothelium-dependent NO-mediated dilation in coronary arterioles by producing superoxide from NAD(P)H oxidase via p38 kinase activation. PMID: 15718491
  4. The present study shows that elevated levels of serum CRP and IL-10 were associated with porcine circovirus type 2-infected piglets that subsequently developed severe PMWS. (postweaning multisystemic wasting syndrome) PMID: 16817761
  5. These data suggest that CRP might play a pathogenic role in the development of myointimal hyperplasia in polytetrafluoroethylene grafts. PMID: 17510097
  6. myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP PMID: 19036073
  7. CRP stimulates superoxide production and the subsequent formation of peroxynitrite from basal released nitric oxide compromises PGI(2) synthesis. PMID: 19410579
  8. Data show that during posttranplant obliterative bronchiolitis development, CRP is localized in inflammatory cells, myofibroblasts and endothelial cells probably as a part of the local inflammatory response. PMID: 19503785

Show More

Hide All

Subcellular Location Secreted
Protein Families Pentraxin family
Tissue Specificity Found in plasma.
Database Links

KEGG: ssc:396842

STRING: 9823.ENSSSCP00000029570

UniGene: Ssc.16157

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1