Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

Code CSB-E10373Rb
Size 96T,5×96T,10×96T
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Product Details

Target Name
matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Alternative Names
MMP2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
Abbreviation
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 89  
Range % 85-95  
1:20 Average % 96  
Range % 90-105  
1:40 Average % 93  
Range % 85-99  
1:80 Average % 94  
Range % 86-98  
Recovery
The recovery of rabbit MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-103  
EDTA plasma (n=4) 92 87-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.506 2.531 2.519 2.419  
50 2.048 2.005 2.027 1.927  
25 1.674 1.691 1.683 1.583  
12.5 1.184 1.207 1.196 1.096  
6.25 0.882 0.879 0.881 0.781  
3.12 0.554 0.543 0.549 0.449  
1.56 0.306 0.314 0.310 0.210  
0 0.102 0.097 0.100    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit MMP2 ELISA Kit was designed for the quantitative measurement of Rabbit MMP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

Function
(From Uniprot)
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels.
Gene References into Functions
  1. These results show that MMP-2 activates the EGFR and triggers downstream signaling pathways increasing Reactive Oxygen Species formation and promoting vasoconstriction. PMID: 30029165
  2. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
  3. Selenium suppressed high-fat diet-induced MMP2 over-expression in vivo by improving lipid metabolism. PMID: 28595793
  4. Repetitive mechanical stimulation of tendon cells results in a small increase in matrix metalloproteinase2 levels, but it appears unlikely that serum matrix metalloproteinase2 will be a useful indicator of tendon overuse injury. PMID: 26909661
  5. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
  6. Results provide evidence that MMP-2 bears the potentiality to cleave alpha-DG enriched from rabbit skeletal muscle indicating that this degradation indeed might also occur in vivo. PMID: 25483986
  7. These data suggest that OxyHb suppresses K V currents through both reactive oxygen species-dependent and independent pathways involving MMP activation. PMID: 25366605
  8. In conclusion, MMP-2 could be responsible for the proteolysis of dystrophin. PMID: 24791785
  9. Castor oil polymer induces bone formation with high matrix metalloproteinase-2 expression. PMID: 23670892
  10. MMP2 spinal cord expression is increased in cervical spondylotic myelopathy. PMID: 22673181
  11. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
  12. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
  13. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
  14. The RNA interference targeting COX-2 can effectively inhibit the expression of COX-2 and MMP-2 in IL-1alpha stimulated rabbit corneal stromal cells in vitro. PMID: 19175164
  15. Our results strongly suggest that ischaemic postconditioning may exert part of its cardioprotective effects through the inhibition of MMP-2 activity. PMID: 19880538

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Membrane. Nucleus.
Protein Families
Peptidase M10A family
Database Links
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