Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

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Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

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Citations (6) Protocol MSDS

Code	CSB-E07419r
Size	96T,5×96T,10×96T
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Product Details
Citations (6)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

chemokine (C-X-C motif) ligand 2

Alternative Names

Cxcl2 ELISA kit; Cinc3 ELISA kit; Mip-2 ELISA kit; Mip2 ELISA kit; Scyb2C-X-C motif chemokine 2 ELISA kit; Cytokine-induced neutrophil chemoattractant 3 ELISA kit; CINC-3 ELISA kit; Macrophage inflammatory protein 2 ELISA kit; MIP2 ELISA kit

Abbreviation

CXCL2

Uniprot No.

P30348

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-95
1:2	Average %	90
	Range %	85-99
1:4	Average %	98
	Range %	94-101
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.899

2.934

2.916

2.778

2500

2.437

2.567

2.502

2.364

1250

1.817

1.828

1.822

1.684

625

1.228

1.229

1.091

312

0.736

0.721

0.729

0.591

156

0.445

0.453

0.449

0.311

0.263

0.254

0.259

0.121

0.141

0.135

0.138

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The rat MIP-2 ELISA Kit is intended to measure rat MIP-2 in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify rat MIP-2 concentration. The intensity of the colored product is directly proportional to the concentration of MIP-2 present in the sample. This rat MIP-2 ELISA kit has high specificity and excellent sensitivity.

MIP-2, also called CXCL2, is a peptide derived from monocytes/ macrophages that attracts and recruits neutrophils to inflamed sites through the p38 MAPK-dependent signaling pathway, by binding to CXCR1 and CXCR2. CXCL2-recruited and activated neutrophils can accelerate liver inflammation by releasing various inflammatory mediators. CXCL2 expression is increased during hepatic I/R injury. Some studies have demonstrated that CXCL2 is overexpressed in breast, colon, prostate, and bladder cancers. While CXCL2 is down-regulated in HCC cell lines, inhibiting hepatocellular carcinoma (HCC) cell proliferation in vitro.

Rutin attenuates bleomycin-induced acute lung injury via miR-9-5p mediated NF-κB signaling inhibition: network pharmacology analysis and experimental evidence FS Alatawi, AME Omran, E Rashad,Frontiers in Pharmacology,2025
Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary W Lu,American journal of respiratory cell and molecular biology,2022
Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
Annexin V homodimer protects against ischemia reperfusion-induced acute lung injury in lung transplantation Hashimoto K.et al,J Thorac Cardiovasc Surg.,2016
Endotoxin-induced lung alveolar cell injury causes brain cell damage Rodrguez-Gonzlez R.et al,Exp Biol Med (Maywood).,2015
Annexin V Homo-dimer Protects against Ischemia Reperfusion-Induced Acute Lung Injury in Lung Transplantation Kohei Hashimoto. et al,The Journal of Thoracic and Cardiovascular Surgery,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

Gene References into Functions

this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

At least expressed in the lung and trachea.

Database Links

KEGG: rno:114105

STRING: 10116.ENSRNOP00000003745

UniGene: Rn.10230

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Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway TNF signaling pathway IL-17 signaling pathway NOD-like receptor signaling pathway

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