Rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit

Code CSB-E06933Rb
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Alternative Names
MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
Abbreviation
MMP9
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 99  
Range % 96-102  
1:40 Average % 85  
Range % 92-99  
1:80 Average % 101  
Range % 98-104  
1:160 Average % 85  
Range % 82-89  
Recovery
The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 93-99  
EDTA plasma (n=4) 100 96-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1500 0.129 0.127 0.128    
750 0.226 0.238 0.232    
375 0.379 0.370 0.375    
187.5 0.572 0.565 0.569    
94 0.799 0.785 0.792    
47 0.937 0.987 0.962    
23.5 1.148 1.168 1.158    
0 1.606 1.634 1.620    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit MMP9 ELISA Kit was designed for the quantitative measurement of Rabbit MMP9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Gene References into Functions
  1. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
  2. Expression of MMP-9 increased after cerebral aneurysm induction, peaking at week 3, leading to reduced smooth muscle cell number, damaged endothelial cells, and damage to the aneurysm wall elastic layer. PMID: 24534031
  3. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
  4. Therefore, it was reasonable to speculate that the increased expression of VEGF and MMP-9 in residual hepatic tumor cells and tumor angiogenesis post-embolization would be responsible for the increased metastatic potentiality and invasiveness. PMID: 23392714
  5. Performing minimally invasive surgical procedures in the early stages of intracerebral hemorrhage significantly decreases MMP-9. PMID: 23561043
  6. Increased expression of MMP-9 in spinal cord follows cervical spondylotic myelopathy. PMID: 22673181
  7. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
  8. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
  9. In experimental syringomyelia, MMP-9 plays an important role in causing edema in the presyrinx state. PMID: 21092471
  10. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818
  11. MMP promoter activation occurs as a function of thoracic aorta wall tension in transgenic mice PMID: 19375723
  12. TGF-beta mediated MMP-9 induction may be regulated by the NF-kappaB, Smad3, and JNK pathways, whereas the IL-1beta mediated induction may be regulated by the NF-kappaB and p38 pathways. PMID: 19626678

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Osteoclasts.
Database Links
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