Recombinant Human Oncostatin-M protein(OSM) (Active)

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Code CSB-AP002231HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
Target Names OSM
Uniprot No. P13725
Research Area Immunology
Alternative Names MGC20461; ONCM_HUMAN; Oncostatin M; Oncostatin-M; OSM
Species Homo sapiens (Human)
Source E.coli
Expression Region 26-220aa
Complete Sequence AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSR
Mol. Weight 22.0 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).
Gene References into Functions
  1. The mechanism of prostaglandin E2-induced transcriptional up-regulation of Oncostatin-M by CREB and Sp1 has been described. PMID: 29269396
  2. OSM [oncostatin M]might be involved in the invasiveness of extravillous trophoblasts under hypoxia conditions via increasing MMP-2 and MMP-9 enzymatic activities through STAT3 signaling. Increased MMP-9 activity by OSM seems to be more important in primary trophoblasts. PMID: 30091322
  3. IL6 family cytokine oncostatin-M (OSM) induced a switch to the EMT phenotype and protected cells from targeted drug-induced apoptosis in OSM receptors (OSMRs)/JAK1/STAT3-dependent manner PMID: 28729401
  4. Oncostatin M induces RIG-I and MDA5 expression and enhances the double-stranded RNA response in fibroblasts. PMID: 28560754
  5. The IL-6-type cytokine oncostatin M (OSM) indeed induces cellular properties associated with tumorigenesis and disease progression in non-transformed human prostate epithelial cells, including morphological changes, epithelial-to-mesenchymal transition (EMT), enhanced migration and pro-invasive growth patterns. PMID: 29526757
  6. downregulation of miR-20a-5p is caused by promoter hypermethylation. MiR-20a-5p could also suppress the production of IL-17 by targeting OSM and CCL1 production in CD4(+) T cells in patients with active VKH. PMID: 28972028
  7. our findings suggested that OSM suppresses SLUG expression and tumor metastasis of lung adenocarcinoma cells through inducing the inhibitory effect of the STAT1-dependent pathway and suppressing the activating effect of STAT3-dependent signaling PMID: 27486982
  8. Genistein (a specific Tyr phosphorylation inhibitor) leads to the interaction of CHOP (C/EBP Homologous Protein) with C/EBP-beta, thus negatively regulating it. Knockdown of C/EBP-beta also leads to inhibition of PMA-mediated OSM induction. PMID: 27676154
  9. Data provide evidence that OSM regulates an epithelial-mesenchymal transition and cancer stem cell plasticity program that promotes tumorigenic properties in pancreatic cells. PMID: 28053127
  10. OSM-induced plasticity was Signal Transducer and Activator of Transcription 3 (STAT3)-dependent, and also required a novel intersection with transforming growth factor-beta (TGF-beta)/SMAD signaling. Removal of OSM or inhibition of STAT3 or SMAD3 resulted in a marked reversion to a non-invasive, epithelial phenotype. PMID: 28288136
  11. Neutrophils are a major source of OSM-producing cells in patients with chronic rhinosinusitis and severe asthma. PMID: 27993536
  12. OSM and OSMR are highly expressed in inflammatory bowel disease intestinal mucosa compared to control mucosa. OSM promotes inflammatory behavior in human intestinal stroma. PMID: 28368383
  13. Study showed that in atrial fibrillation (AF) with thrombus, the atrial tissue infiltration of M1 macrophages increased significantly; the OSM expression was also found to increase simultaneously; downstream tissue factor (TF) increased and tissue factor pathway inhibitors (TFPI)decreased, leading to an imbalance between TF and TFPI eventually. OSM might be related to thrombosis in patients with AF mediated by TF and TFPI PMID: 28471981
  14. a novel STAT3/SMAD3-signaling axis is required for OSM-mediated senescence. PMID: 27892764
  15. This result demonstrates that HPV16 oncoproteins upregulate oncostatin M and play an important role to promote oral squamous cell carcinoma development PMID: 27349249
  16. The identification of the OSM inflammatory pathway as an important mediator of epithelial mesenchymal transition in triple-negative breast cancer (TNBC) may provide a novel potential opportunity to improve therapeutic strategies. PMID: 28106823
  17. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. PMID: 26198770
  18. Oncostatin M can regulate airway smooth muscle responses alone or in synergy with IL-17A. PMID: 25849622
  19. we demonstrated that recombinant human OSM (rhOSM) promoted tumor angiogenesis in EC cell lines by activating STAT3 (signal transducer and activator of transcription 3) and enhanced both cell migration and cell inva PMID: 25954856
  20. OSM expression in osteoblasts increases in response to Osteopontin-induced inflammation in vitro. PMID: 26304992
  21. Data suggest that OSM promotes osteoblastic differentiation of vascular smooth muscle cells through JAK3/STAT3 pathway and may contribute to the development of atherosclerotic calcification. PMID: 25735629
  22. administration of Fstl1 induced airway remodeling and increased OSM, whereas administration of an anti-OSM Ab blocked the effect of Fstl1 on inducing airway remodeling, eosinophilic airway inflammation PMID: 26355153
  23. OSM promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. PMID: 25840724
  24. Oncostatin M regulates neuronal function and confers neuroprotectin in an animal model of ischemic stroke. PMID: 26311783
  25. In patients with diabetes, bone marrow plasma OSM levels were higher and correlated with the bone marrow to peripheral blood stem cell ratio. PMID: 25804939
  26. OSM promotes STAT3-dependent intestinal epithelial cell proliferation and wound healing in vitro. PMID: 24710357
  27. Autocrine activation of STAT3 in MCF-7 cells ectopically expressing OSM-induced cellular scattering. PMID: 25252914
  28. oncostatin M is a cytokine possessing vigorous antiviral and immunostimulatory properties which is released by APC upon interaction with CD40L present on activated CD4+ T cells. PMID: 24418171
  29. Data indicate that pro-inflammatory cytokines such as IL6 or OSM could activate pathways associated with prostate cancer progression and synergize with cell-autonomous oncogenic events to promote aggressive malignancy. PMID: 23867565
  30. OSM may promote a clinically relevant EMT/CSC-like phenotype in human breast cancer via a PI3K-dependent mechanism PMID: 23584474
  31. white adipose tissue macrophages are a source of OSM and OSM levels are significantly induced in obesity/type 2 diabetes. OSM produced from immune cells in WAT may act in a paracrine manner on adipocytes to promote inflammation in adipose tissue. PMID: 24297795
  32. data suggest that increased serum OSM levels are associated with the coronary stenosis score and that circulating levels of this chemokine may reflect the extent of coronary atherosclerosis PMID: 24600984
  33. TGFBI and periostin, extracellular matrix proteins implicated in tumorigenesis and metastasis, were identified as oncostatin M-induced secreted proteins in mesenchymal stem cells. PMID: 23735324
  34. Oncostatin M is a FIP1L1/PDGFRA-dependent mediator of cytokine production in chronic eosinophilic leukemia. PMID: 23621172
  35. These data show that OSM and IL-1beta are not only a biological characteristic signature of hypertensive leg ulcer, but these cytokines reflect a specific inflammatory state, directly involved in the pathogenesis. PMID: 23313749
  36. OSM induced proliferation of Ewing sarcoma cell lines. PMID: 22982441
  37. Data suggest that OSM enhances invasion activities of extravillous trophoblasts during placentation through increased enzyme activity of MMP-2 (primarily) and MMP-9 (to some extent). PMID: 22931588
  38. A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. PMID: 22829597
  39. Oncostatin M signaling may cause suppression of estrogen receptor-alpha and disease progression i breast cancer. PMID: 22267707
  40. Oncostatin M (OSM), a cytokine of the IL-6 family, was identified as a major coupling factor produced by activated circulating CD14+ or bone marrow CD11b+ monocytes/macrophages. PMID: 22267310
  41. Oncostatin M (OSM) is a major mediator of cardiomyocyte dedifferentiation and remodeling during acute myocardial infarction (MI) and in chronic dilated cardiomyopathy (DCM). PMID: 22056139
  42. JAK2 V617F-mediated up-regulation of OSM may contribute to fibrosis, neoangiogenesis, and the cytokine storm observed in myeloproliferative neoplasms. PMID: 22051730
  43. c-MYC is an important molecular switch that alters the cellular response to OSM-mediated signaling from tumor suppressive to tumor promoting. PMID: 21975934
  44. A possible interaction between IL-6, OSM, u-PA and VEGF in prostate cancer was investigated. PMID: 21965736
  45. This report uses an in vitro model with human umbilical vein endothelial cells and isolated human neutrophils to examine the effects of two locally derived cytokines, granulocyte-macrophage colony-stimulating factor and G-CSF, on oncostatin M expression. PMID: 21775705
  46. OSM is expressed in atherosclerotic lesions and may contribute to the progression of atherosclerosis by promoting SMC proliferation, migration and extracellular matrix protein synthesis through the STAT pathway PMID: 21376322
  47. Taken together, our data show that KIT D816V promotes expression of OSM through activation of STAT5. PMID: 21457934
  48. The purpose of this study was to investigate the possible suppressive or stimulatory role of OSM in the ovarian cancer model of SKOV3 cells, as well as the involvement of the ERK1/2, p38 and STAT3 signaling pathways. PMID: 21399864
  49. promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines PMID: 21481226
  50. a cytokine-triggered regulatory network for PCSK9 expression that is linked to JAKs and the ERK signaling pathway PMID: 21196532
  51. The expression and secretion of oncostatin M and of its receptor were strongly up-regulated by Helicobacter pylori or by Helicobacter pylori culture supernatant. Oncostatin M secretion was independent of the CagA, VacA or Type IV secretion system. PMID: 20661773
  52. elevated expression in kidneys from patients with urinary obstruction PMID: 20626292
  53. OSM, and to a lesser degree IL-6, induces the expression of Grp78/BiP, an ER chaperone associated with tumor development and poor prognosis in cancer. PMID: 20650266
  54. The greater the amount of periodontal tissue destruction there is substantial increase in gingival crevicular fluid OSM concentrations. PMID: 20189410
  55. Findings provide evidence that the inflammatory cytokine oncostatin M is involved in modulation of the Ang-Tie system by increasing Ang2 expression in human endothelial cells in vitro, and in murine hearts and human hearts in vivo. PMID: 20088942
  56. oncostatin M is a gp 130 cytokine inflammatory biomarker that has a role in periodontal disease PMID: 20408777
  57. Inflammatory cytokines oncostatin-M and interleukin-6 can regulate S100A7 expression and that S100A7 may mediate some of their effects in breast cancer. PMID: 20101226
  58. OSM may play a biological marker for severe preeclampsia, and its action may be predominantly on the trophoblasts and endothelium of placenta villi in preeclampsia. PMID: 20149034
  59. Sputum oncostatin M is increased in asthma with irreversible airflow obstruction and is present in airway neutrophils and macrophages PMID: 19916861
  60. We conclude that STAT1 activation is necessary but not sufficient for induction of transcription of IFN gamma-responsive genes; signals provided by IFN gamma other than STAT1 activation cannot be provided in trans to complement the response to OnM. PMID: 11777927
  61. Oncostatin M and leukemia inhibitory factor regulate the growth of normal human breast epithelial cells PMID: 11811789
  62. Il-6 and oncostatin M act synergistically to promote growth in a new human myeloma cell line. PMID: 11818668
  63. role in identifying sterol-indendent regulatory elements in human ATP-binding cassette transporter A1 promoter PMID: 11839742
  64. role in inducing alpha1-antitrypsin gene expression PMID: 11936950
  65. expression and evidence for STAT3 activation in human ovarian carcinomas PMID: 12061840
  66. produced in human dendritic cells in response to bacterial products PMID: 12061841
  67. Oncostatin M induces tissue-type plasminogen activator and plasminogen activator inhibitor-1 in lung tumor cells PMID: 12090757
  68. Oncostatin M induces procoagulant activity in human vascular smooth muscle cells by modulating the balance between tissue factor and tissue factor pathway inhibitor. OSM might be involved in the thrombotic complications associated with plaque rupture. PMID: 12138373
  69. Oncostatin M stimulates (45)Ca release and enhances mRNA expression of receptor activator of NF-kappa B ligand (RANKL) and osteoprotegerin in neonatal mouse calvarial bone cultures, but has no effect on the expression of RANK. PMID: 12218157
  70. Raised intraperitoneal levels of OSM during bacterial infections originate from infiltrating neutrophils and regulate mesothelial expression of inflammatory cytokines. PMID: 12391243
  71. Kaposi sarcoma-associated viral cyclin K overrides cell growth inhibition mediated by this protein through STAT3 inhibition. PMID: 12531804
  72. Increased production of this protein is found in lymphomononuclear cells from HIV-1-infected patients with neuroAIDS. PMID: 12640208
  73. oncostatin M, which transmits its signal via the gp130 cell surface receptor, and results in the selective down-modulation of the melanocyte lineage antigens PMID: 12692260
  74. Leukemia inhibitory factor (LIF), cardiotrophin-1, and oncostatin M share structural binding determinants in the immunoglobulin-like domain of LIF receptor PMID: 12707269
  75. this important cytokine is released from neutrophils as they infiltrate rheumatoid joints and, thus, contribute to the complex cytokine network that characterizes rheumatoid arthritis PMID: 15146412
  76. IL-6 stimulates proliferation of prostate cancer 22Rv1 cells, in part through activation of the phosphatidylinositol 3-kinase (PI 3-K) signaling pathway. PMID: 15712220
  77. Effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. PMID: 15809742
  78. The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles. PMID: 15831292
  79. IL-6 and OSM upregulate PAI-1 protein and mRNA in adipose tissue PMID: 15837947
  80. OSM may play a role in modulating the inflammatory cascade of chronic periodontitis. PMID: 15863389
  81. Oncostatin M is expressed in the human nasal mucosa and is upregulated in the setting of allergic nasal inflammation. PMID: 16369169
  82. OSM induces a motile/invasive phenotype in T-47D human breast cancer cells in vitro; OSM may enhance metastasis in vivo. PMID: 16713283
  83. Pre-B cell colony-enhancing factor (PBEF) is regulated via IL-6 trans-signaling and the IL-6-related cytokine OSM. PBEF is also actively expressed during arthritis. PMID: 16802343
  84. data suggest that OSM promotes angiogenesis and endothelial cell migration and potentiates the effects of IL-1beta in promoting extracellular matrix turnover and human cartilage degradation PMID: 17009243
  85. sOSMR is able to bind OSM and interleukin-31 when associated to soluble gp130 or soluble interleukin-31R, respectively, and to neutralize both cytokine properties PMID: 17028186
  86. These results suggest that OSM inhibits adiponectin expression by inducing dedifferentiation of adipocytes through signaling pathways involving JAK3 and MEK, but not JAK2. PMID: 17081797
  87. OSM and its receptor play an important role in cutaneous inflammatory responses in general and the specific effects of OSM are associated with distinct inflammatory diseases depending on the cytokine environment. PMID: 17372020
  88. in addition to growth arrest and induced differentiation, OSM also sensitizes normal and transformed osteoblasts to apoptosis by a mechanism implicating (i) activation and nuclear translocation of STAT5 and p53 and (ii) an increased Bax/Bcl-2 ratio. PMID: 17471233
  89. If the effect of oncostatin M on PAI-1 in smooth muscle cells is operative in vivo, it could, via fibrinolysis and proteolysis, be involved in plaque progression, destabilization, thrombus formation, restenosis, and neointima formation. PMID: 17604327
  90. Oncostatin M directly lowers the plasma triglycerides in hyperlipidemia by stimulating the transcription of ACSL3/5 in the liver. PMID: 17761945
  91. In human proximal tubular cells ERK1/2 signaling represents an important component of oncostatin M inhibitory effect on N-cadherin expression. PMID: 17881458
  92. Increased expression of some IL-6 cytokine family members (oncostatin M, gp130, CT-1, LIF) in cutaneous inflammation might contribute to the promotion of hair loss. PMID: 17979974
  93. Production of OSM by human mast cells might represent one link between T cell-induced mast cell activation and development of spectrum of structural changes in T cell-mediated inflammatory processes in which mast cells have been found to be involved. PMID: 18028996
  94. Our data establish a link between the complement system and the gp130 receptor cytokine family with possible implications for the pathology of inflammatory diseases. PMID: 18187666
  95. secretion of OSM and LIF by both epithelial and stromal (paracrine manner) cells seems to promote tumor growth in human breast carcinoma PMID: 18317962
  96. Mtb infection of monocytes results in prostaglandin-dependent OSM secretion, which synergizes with TNF-alpha to drive functionally unopposed fibroblast MMP-1/-3 secretion. PMID: 18398932
  97. loss of repopulating activity during KIT-ligand stimulation is counteracted by Oncostatin M through the downregulation of ERK pathway signaling PMID: 18499891
  98. Oncostatin M is expressed in both chronic obstructive sialadenitis and normal submandibular gland, and is up-regulated in chronic obstructive sialadenitis. PMID: 18564531
  99. expressed in epithelialized apical periodontitis lesions PMID: 18637848
  100. hXOR is a tumor suppressor-targeted gene and the phosphorylation of SAFB1 is regulated by OSM, which provides a molecular basis for understanding the role of SAFB1-regulated hXOR transcription in cytokine stimulation and tumorigenesis PMID: 18772145

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Subcellular Location Secreted
Protein Families LIF/OSM family
Database Links

HGNC: 8506

OMIM: 165095

KEGG: hsa:5008

STRING: 9606.ENSP00000215781

UniGene: Hs.248156

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