Recombinant Human Somatotropin protein(GH1) (Active)

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Code CSB-AP000011HU
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Product Details

Purity >98% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Target Names GH1
Uniprot No. P01241
Research Area Signal Transduction
Alternative Names gH; GH-N; GH1; GHB5; GHN; Growth hormone 1; Growth hormone; Growth hormone B5; Growth hormone; normal; Growth hormone; pituitary; HG1; hGH-N; IGHD1B; Pituitary growth hormone; RNGHGP; SOMA_HUMAN; Somatotropin
Species Homo sapiens (Human)
Source E.Coli
Expression Region 27-127aa
Complete Sequence FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F
Mol. Weight 22.0 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Gene References into Functions
  1. Significant correlations were observed between GH concentration and impairments on several EDI-2 subscales (drive for thinness, body dissatisfaction, interoceptive awareness, sense of ineffectiveness, interpersonal distrust, maturity fear) and on SCL-90 subitems (depression, hostility, obsessivity compulsivity, anxiety), suggesting a possible hormonal modulatory effect on specific aspects of eating disorders. PMID: 29179911
  2. Patients examined at 1 year or several years from complicated mild traumatic brain injury had a similarly high occurrence of isolated GH deficiency PMID: 27878771
  3. Single nucleotide variant in GH1 gene is associated with isolated growth hormone deficiency. PMID: 28910730
  4. In newborns, serum PRL and hGH levels show a positive correlation that can be explained by common regulatory factors or a drift phenomenon. A higher gestational week is associated with a higher PRL/hGH ratio. PMID: 28700563
  5. This review describes the endocrine profile of centenarians concerning the GH/IGF-I/insulin system, focusing on the relevance of this pathway on the modulation of ageing and longevity. PMID: 27932301
  6. Data suggest that patients with NICTH (non-islet cell tumor hypoglycemia) exhibit low serum growth hormone levels during hypoglycemic episodes. [Retrospective Study & REVIEW of Case Reports] PMID: 28529277
  7. Homozygous GH1 deletion is associated with growth hormone deficiency. PMID: 28525353
  8. Human Growth Hormone Inhibits CLAUDIN-1 Expression Through Activation of Signal Transducer and Activator of Transcription 3 (STAT3). PMID: 28617312
  9. Serum GH was unrelated to type 2 diabetes, fasting blood glucose, or HbA1c level. PMID: 27060213
  10. GH1 and GHRHR screening revealed eleven variations in 24 (21%) patients with isolated growth hormone deficiency of which, four were novel deleterious, one novel non-pathogenic and six reported changes. PMID: 27114065
  11. The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. PMID: 27150070
  12. These results implicate TIMP3 as a modulator of cell surface GHR abundance and the ability of GH to promote cellular signaling. PMID: 27075707
  13. Children with GH excess underwent medical treatment with lanreotide and a minimum clinical/biochemical follow up of 2 years is reported. The present study demonstrates that GH excess should be considered as a relative frequent endocrine manifestation in NF1 patients, similarly to central precocious puberty PMID: 28631895
  14. Thus, GHRH analogs of the Miami series powerfully suppress tumor growth, but have only a weak endocrine GH inhibitory activity. The suppression of tumor growth could be induced in part by the downregulation of GHRH receptors levels. PMID: 28130121
  15. the levels and kinetics of phosphorylation mediated by the main signalling proteins triggered by 22K-GH or 20K-GH were not exactly the same. PMID: 28427901
  16. A negative regulation of locally produced GH by androgens/AR in Prostate cancer cells following treatment with AR agonists (R1881) and antagonists (enzalutamide, bicalutamide). PMID: 28444169
  17. The intrinsic amyloidogenicity of growth hormone, in the presence of contaminating prion protein (and perhaps prolactin as well) and amyloid-beta contained in some cadavers' pituitaries, may have led to the observed co-occurring of Creutzfeldt-Jakob disease and Alzheimer's disease. PMID: 27214308
  18. GH potentially negatively modulates the maturation and accumulation of lipid in adipocytes. PMID: 27802441
  19. To our knowledge, c.-223C>T is the first homozygous point mutation in the GH1 promoter that leads to short stature due to idiopathic growth hormone deficiency. PMID: 27252485
  20. Data show that the recombinant protein produced by the plasmid-free E coli strain was purified and characterized to be human growth hormone (hGH). PMID: 27542624
  21. Our results suggest that the known protective effect of GH signaling deficiency on neoplastic tissue growth is mediated, at least partially, by regulating p53 expression PMID: 27226307
  22. evidence that hGH synthesis follows a diurnal rhythm and of dynamic associations of the circadian machinery with a component of a chromosomal structure of the hGH1 locus that is essential for efficient expression. PMID: 27151213
  23. Gene polymorphism of leptin (loci rs7799039) and leptin receptor (loci rs1137101) are correlated with Growth hormone deficiency susceptibility. PMID: 26915772
  24. These results showed that hybrid training system on a cycle ergometer (CE) was more efficient in stimulating acute increases in GH, lactate and IL-6 than CE at the same workload. PMID: 26522057
  25. Growth Hormone 1 T1663A Polymorphism were at a decreased risk of breast cancer. PMID: 26225688
  26. This is the first report of a family suffering from short stature caused by autosomal dominant form of GH deficiency II, which severely affects intracellular GH folding and stability as well as secretion PMID: 26485222
  27. GH and IGF-1 suppression is maintained for up to 25 months during pasireotide LAR treatment of acromegaly. PMID: 25103549
  28. In women with normal somatotroph function, GH levels do not change in the first trimester of pregnancy. PMID: 25179796
  29. These data on pregnancy outcomes in a large group of women with hypopituitarism revealed no relationship between GH replacement therapy regimens and pregnancy outcomes. PMID: 26256649
  30. Effect of oral glucose administration on rebound growth hormone release in normal and obese women: the role of adiposity, insulin sensitivity and ghrelin. PMID: 25782001
  31. the phenotype of MIP-FoxM1-hGH mice is due primarily to hGH activity and that the FoxM1 protein remains largely inactive PMID: 26202070
  32. Case Report: of Klinefelter sydnrome with short stature due to growth hormone deficiency. PMID: 25241616
  33. Suggest growth hormone deficiency may be common feature in vernal keratoconjuntivitis patients. PMID: 25079463
  34. Human Growth Hormone stimulates the microRNA 96-182-183 cluster, which promotes the epithelial-mesenchymal transition and invasion in breast cancer PMID: 25873390
  35. The results demonstrate that activation of noncoding transcription reflects an autonomous activity of the human growth hormone long-range enhancer that is fully independent of interactions with linked gene promoters and occurring in spatial and temporal synchrony with initiation of GH expression in the embryonic pituitary. PMID: 25662214
  36. Analysis of GH1 in a cohort of Brazilian patients revealed that the autosomal recessive form of isolated growth hormone deficiency(IGHD) was more common than the dominant one, and both were found only in severe IGHD. PMID: 25116472
  37. Recipients showed a rapid recovery of the GH/IGF1 hormonal axis and liver function after LDLT, whereas donors showed altered GH signaling and regenerative delay in the early days after living donation. PMID: 24889799
  38. JAK2 is activated by growth hormone and other cytokines. (Review) PMID: 25656053
  39. Genotyping contributed to the diagnosis of children with suspected growth hormone insensitivity and short stature. PMID: 25411237
  40. hGH production is extremely sensitive to increased caloric intake. PMID: 25295535
  41. After LT, GH levels correlate with the extent of cytolysis, while IGF-1 is an indicator of liver synthetic function recovery. IGF-1 levels >90 mug/L (day 15-30) seem to be an indicator of short-term survival. PMID: 24804205
  42. In this review we highlight the evidence of extrapituitary synthesis of GH in humans. [review] PMID: 24642386
  43. This review summarizes findings of growth hormone's influence on in utero and neonatal cellular and metabolic profiles related to bone and adipose tissue. PMID: 25015810
  44. The study presents experimental data for the mechanism of thiol-disulfide exchange in tryptic peptides derived from human growth hormone in aqueous solution. PMID: 24549831
  45. we present the results of screening for mutations in GH1 and GHRHR genes in a large cohort of Argentinian patients with IGHD. These suggest that the p.Arg183His mutation associated with the type II dominant form of IGHD might be relatively common. PMID: 23789946
  46. In the first family a novel splice site mutation in GH1 was identified (c.172-1G>C, IVS2-1G>C). In two other families a previously reported splice site mutation (c.291+1G>A, IVS3+1G>A) was found. PMID: 24280736
  47. Bilateral involvement of a pituitary adenoma and severely decreased immediate postoperative serum GH levels at 72 hours after transsphenoidal adenomectomy may be independent risk factors for accelerated GH deficiency in acromegalic patients. PMID: 24972779
  48. The activity of autocrine GH may be distinct from that of endocrine GH in prostate cancer cells. PMID: 23238889
  49. robust GH-stimulated hepatic Igf1 gene transcription utilizes tissue-specific mechanisms of epigenetic regulation that are established independent of GH signaling. PMID: 24109593
  50. meta-analysis indicates that GH1 T1663A polymorphism may contribute to the risk of colorectal cancer, especially among Asian populations PMID: 24464925
  51. Data from 3 male subjects with isolated growth hormone deficiency, type II, suggest 3 additional mutations in exon 3 of GH1 can be responsible for this disease; these data support novel putative splicing regulatory elements within exon 3. PMID: 24635352
  52. The aim of the present study was to evaluate GH responses to repeated bouts of respiratory muscle endurance training in obese adolescents and adults. PMID: 23700319
  53. Case Report: growth hormone deficiency is related to the underlying metabolic disorder in Gaucher disease. PMID: 20049531
  54. GH resistance is an adaptive response to states of undernutrition. PMID: 24363451
  55. Hypothalamic-pituitary-adrenal axis hyperresponsiveness and a less frequent suppression of the GH/IGF-1 axis in response to acute stress are possibly involved in delirium pathophysiology PMID: 23864592
  56. We observed a significant expressional increase of GH gene in GH4 cells stimulated by bFGF PMID: 23822620
  57. human metastatic melanoma tumors express GHR. PMID: 24134847
  58. Data suggest that, in addition to GHRH (growth hormone releasing hormone) and somatostatin, other factors (such as ghrelin) play roles in regulation of GH release from pituitary and can have effects on musculoskeletal function in aging. [REVIEW] PMID: 23702397
  59. Estradiol can amplify GH intracellular signaling in human osteoblasts with an essential role played by the reduction of the SOCS2 mediated feedback loop. PMID: 23567159
  60. Data suggest del32-71 GH-mediated endoplasmic reticulum (ER) stress contributes to decrease in wild-type GH secretion observed in GH deficiency due to the GH1 gene slice-site mutation; induction of del32-71 GH causes ER stress leading to apoptosis. PMID: 23736291
  61. Data suggest that GH potentiates 17beta-estradiol effects on proliferation in breast cancer cells expressing high levels of GH receptor; GH can overcome antiproliferative effects of insulin-like growth factor I receptor tyrosine kinase inhibition. PMID: 23782942
  62. AMH levels are reduced and estratiol levels are increased in healthy male volunteers treated with growth hormone and a growth hormone antagonist. PMID: 23785020
  63. These results showed that sleep restriction increases the proinflammatory cytokine, GH, and testosterone concentrations after physical exercise but did not affect the cortisol responses. PMID: 23281720
  64. Idiopathic short stature patients with low-normal BMI SDS also tend to display a relative IGF-1 resistance, whereas those with normal BMI SDS tend to be less GH-sensitive than healthy peers PMID: 23419423
  65. Data suggest that GH secretion in patients with acromegaly remains under central control (hypothalamo-hypophyseal system), i.e., nyctohemeral rhythmicity, sexual dimorphism, and age-related decline of GH output. PMID: 23640965
  66. Results provide direct evidence for the role of growth hormone in regulating the insulin sensitivity in insulin-resistant patients. PMID: 23452315
  67. The objective of this study was to evaluate the effects of respiratory muscle endurance training on GH and cortisol responses in severely obese patients. PMID: 22972178
  68. in SHOX deficiency subjects an altered GH secretion is not uncommon, and rhGH therapy may be effective in increasing height in most of these patients independent of GH secretion, without causing adverse events of concern. PMID: 23208451
  69. the level of transcriptional enhancement of hGH-N by HSI is directly related to the intensity of HSI-dependent noncoding transcription and is fully independent of the structure of the locally transcribed RNA PMID: 22431516
  70. These results suggest that certain micronutrients such as vitamin C intake are strongly and uniquely associated with stimulated and endogenous spontaneous GH secretion. PMID: 22465725
  71. Testosterone, growth hormone, and insulin-like growth factor 1 showed with distinct delivery patterns depending on the order of strength training exercises. PMID: 23438236
  72. The study demonstrates that conditional deletion of DNase I-hypersensitive site I from the active hGH-N locus in the adult pituitary effectively silences hGH-N expression. PMID: 23428872
  73. In this chapter, an overview of some basic bioinformatics resources that are needed to study metabolic disorders are reviewed and some examples of bioinformatics analysis of human growth hormone gene, protein and structure are provided. PMID: 23182822
  74. Therapies that specifically target this isoform may be useful in patients with GH-1 splicing defects. PMID: 23182825
  75. two siblings diagnosed with partial bioinactive growth hormone syndrome; subjects (adopted Roma children from Hungary) found to have heterozygous P59S mutation at position involved in binding to growth hormone receptor PMID: 23417163
  76. Report growth hormone deficiency in cystinosis. PMID: 22664570
  77. These results showed a significant association between lower serum GH levels and nonalcoholic fatty liver disease. PMID: 22952901
  78. discussion of role of growth hormone and insulin-like growth factor-I in brain/neuron function, development of or resistance to Alzheimer s disease (AD), and possibility of activation of these systems as targets in prevention/treatment of AD [REVIEW] PMID: 22524398
  79. analysis of HIF-1-dependent down-regulation of hGH1 by insulin via chromatin remodeling specifically in the proximal promoter region PMID: 22833680
  80. In this series of 30 Indian familial/nonfamilial isolated growth hormone deficiency patients, frequency of GH-1 gene deletions (12/30) was 40% among nonfamilial, and 54% among familial patients. PMID: 22016154
  81. These data establish HSII as a nonredundant component of the hGH LCR essential for establishment of robust levels of hGH-N gene expression PMID: 22669946
  82. Recombinant human growth hormone increased collagen synthesis and promoted hepatic stellate cell proliferation in liver cirrhosis. PMID: 22672824
  83. Cognitive performance in elderly males is associated with growth hormone secretion, with respect to target detection and speed of responding in conditions of selective attention, short-term memory, and basic processing speed. PMID: 20483505
  84. The present study shows that a 15-minute respiratory muscle endurance training session is capable of stimulating, in healthy subjects, GH and cortisol release, and of suppressing ghrelin secretion. PMID: 22307889
  85. Recombinant human growth hormone is effective to improve final height in renal transplant recipients, without affecting kidney function. PMID: 22278170
  86. It was found that fat mass, insulin and IGF-1 levels are important negative determinants of SHBG levels in young healthy men. PMID: 21711374
  87. Janus kinase (JAK) 2 V617F mutation may have a role in primary thrombocythemia in acromegaly with severe visceromegaly and divergence between growth hormone and insulin-like growth factor-1 concentrations during the follow-up PMID: 22364960
  88. GHBP plays a role in limiting variations of circulating free GH levels PMID: 22386777
  89. mosaicism for a mutation in GH1 in a family with isolated growth hormone deficiency PMID: 21933221
  90. [review] A common polymorphic GH variant consisting of genomic exon 3 deletion or retention has been linked with increased receptor activity due to enhanced signal transduction. PMID: 22322534
  91. Compared with patients with merely GH-secreting adenomas, acromegalic patients with hyperprolactinemia are characterized by an earlier onset of disease, lesser acromegalic features, lower GH levels, but larger tumor sizes. PMID: 22334636
  92. The role of functional IGFBP3 for diagnosis of disturbances of the growth hormone/IGF hormonal axis appears to be limited. PMID: 22318747
  93. Genes involved in skeletal muscle system and cardiovascular system development were induced by growth hormone. PMID: 22072432
  94. These findings do not provide evidence for a substantial influence of these common polymorphisms in the GH/IGF axis on objectively measured physical capability levels in older adults. PMID: 22253814
  95. Endoscopic transsphenoidal pituitary surgery [TS] is a good primary therapeutic option for patients with GH-secreting macroadenoma, resulting in remission of up to 63%; this technique can potentially improve the outcome of TS in these patients. PMID: 21347581
  96. This effect was accompanied by POU1F1-dependent histone acetylation and methylation throughout the minichromosome LCR/hGH-N domain. PMID: 22094313
  97. GH peaked at mid-puberty and IGF-1 in late puberty, P1NP and CTX were highest in mid-puberty compared with early and late puberty. GH, but not IGF-1, correlated with P1NP. PMID: 21535073
  98. GH assessment during standardized aerobic exercise of moderate intensity is a reliable test with high diagnostic accuracy in predicting severe GH deficiency in adults. PMID: 21856788
  99. While the association of intrauterine growth retardation (IUGR) and adult metabolic syndrome (MS), including diabetes, has been well documented, these subjects with normal GH and insulin-resistant diabetes do not have typical manifestations of MS. PMID: 22170795
  100. [review] Given the limitations of the insulin-like growth factor 1 generation test (IGFGT) in the most severe cases of GH insensitivity syndrome (GHIS), the ability of the IGFGT test to detect less severe GHIS is doubtful. PMID: 22048966

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Involvement in disease Growth hormone deficiency, isolated, 1A (IGHD1A); Growth hormone deficiency, isolated, 1B (IGHD1B); Kowarski syndrome (KWKS); Growth hormone deficiency, isolated, 2 (IGHD2)
Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

HGNC: 4261

OMIM: 139250

KEGG: hsa:2688

STRING: 9606.ENSP00000312673

UniGene: Hs.655229

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