Code | CSB-MA004950A1m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | WB:1:1000-1:8000 |
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The CD63 monoclonal antibody is a highly specific tool used to detect the CD63 protein in various human and rabbit samples. It was generated by immunizing mice with recombinant human CD63 protein (103-203aa), followed by the fusion of immunized mouse spleen cells with myeloma cells to produce hybridomas. The hybridomas are selected to culture in the mouse abdominal cavity, leading to the production of the ascites containing CD63 monoclonal antibodies. The resulting CD63 monoclonal antibody was then screened for specificity using a variety of assays, including ELISA, WB, IHC, IF, and FC. Its purity is over 95% using protein G purified.
CD63 protein plays an important role in vesicular trafficking, antigen presentation, and cell signaling. In cells, CD63 protein interacts with other tetraspanins, integrins, and adhesion molecules to regulate the formation and stability of multimeric protein complexes. It is also involved in the internalization and sorting of plasma membrane proteins to lysosomes for degradation, and the fusion of lysosomes with autophagosomes during autophagy.
Applications : WB
Sample type: cells
Review: Representative Western blot image of the total cell lysates and exosomes secreted by primary astrocytes in the medium showing DICAM and CD63 expressions
By Anonymous